Detailed information NTG0431517

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: HB742_RS24310
• Genome accession: NZ_CP050324
• Coordinates: 5250357..5251229 (+)
• Length: 290 a.a.
• NCBI ID: WP_023084740.1
• Uniprot ID: -
• Organism: Pseudomonas aeruginosa strain DVT427
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5245357..5256229

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HB742_RS24295 (HB742_24315)	-	5246740..5247204 (-)	465	WP_023090842.1	pilin	-
HB742_RS24300 (HB742_24320)	pilB	5247435..5249135 (+)	1701	WP_003107297.1	type IV-A pilus assembly ATPase PilB	Machinery gene
HB742_RS24305 (HB742_24325)	pilC	5249139..5250356 (+)	1218	WP_003161763.1	type II secretion system F family protein	Machinery gene
HB742_RS24310 (HB742_24330)	pilD	5250357..5251229 (+)	873	WP_023084740.1	type IV prepilin peptidase/methyltransferase PilD	Machinery gene
HB742_RS24315 (HB742_24335)	coaE	5251226..5251837 (+)	612	WP_003112838.1	dephospho-CoA kinase	-
HB742_RS24320 (HB742_24340)	yacG	5251834..5252034 (+)	201	WP_003094656.1	DNA gyrase inhibitor YacG	-
HB742_RS24325 (HB742_24345)	-	5252071..5252280 (-)	210	WP_020750398.1	hypothetical protein	-
HB742_RS24330 (HB742_24350)	-	5252386..5253075 (-)	690	WP_003103868.1	energy-coupling factor ABC transporter permease	-
HB742_RS24335 (HB742_24355)	-	5253072..5253542 (-)	471	WP_019486073.1	hypothetical protein	-
HB742_RS24340 (HB742_24360)	-	5253539..5253964 (-)	426	WP_003103865.1	GNAT family N-acetyltransferase	-
HB742_RS24345 (HB742_24365)	-	5254097..5254726 (+)	630	WP_003094668.1	DUF1780 domain-containing protein	-
HB742_RS24350 (HB742_24370)	-	5254723..5255172 (+)	450	WP_023084741.1	MOSC domain-containing protein	-
HB742_RS24355 (HB742_24375)	-	5255198..5255368 (+)	171	WP_003094672.1	DUF3094 family protein	-

Sequence

Protein

• Length: 290 a.a.
• Molecular weight: 31843.88 Da
• Isoelectric Point: 7.7068

>NTDB_id=431517 HB742_RS24310 WP_023084740.1 5250357..5251229(+) (pilD) [Pseudomonas aeruginosa strain DVT427]
MTLLDYLASHPLAFVLCAILLGLLVGSFLNVVVHRLPKMMERNWKAEAREALGLEPEPKQATYNLVLPNSACPRCGHEIR
PWENIPLVSYLALGGKCSSCKAAIGKRYPLVELATALLSGYVAWHFGFTWQAGAMLLLTWGLLAMSLIDADHQLLPDVLV
LPLLWLGLIANHFGLFASLDDALFGAVFGYLSLWSVFWLFKLVTGKEGMGYGDFKLLAMLGAWGGWQILPLTILLSSLVG
AILGVIMLRLRNAESGTPIPFGPYLAIAGWIALLWGDQITRTYLQFAGFK

Nucleotide

• Length: 873 bp

>NTDB_id=431517 HB742_RS24310 WP_023084740.1 5250357..5251229(+) (pilD) [Pseudomonas aeruginosa strain DVT427]
ATGACCCTCCTCGACTACCTGGCCAGCCATCCGCTGGCCTTTGTCTTGTGCGCCATCCTCCTTGGCCTGCTGGTCGGCAG
CTTCCTCAACGTCGTCGTGCATCGCCTGCCGAAGATGATGGAGCGCAACTGGAAGGCCGAGGCCCGCGAAGCGCTCGGGC
TGGAGCCGGAACCCAAGCAGGCGACCTACAACCTGGTGCTGCCGAATTCGGCGTGCCCGCGCTGCGGCCACGAGATCCGC
CCCTGGGAAAACATCCCGCTGGTCAGCTACCTGGCGCTGGGCGGCAAGTGCTCGTCCTGCAAGGCCGCCATCGGCAAACG
CTACCCCCTGGTGGAACTGGCCACCGCGCTGCTTTCCGGCTACGTCGCCTGGCATTTCGGCTTCACCTGGCAGGCGGGCG
CGATGCTCCTGCTGACCTGGGGTCTGCTGGCGATGAGCCTGATCGATGCCGATCACCAACTGCTGCCGGACGTGCTGGTG
CTGCCGCTGCTCTGGCTGGGCCTGATCGCCAACCATTTCGGCCTCTTCGCCAGCCTCGACGATGCGCTGTTCGGCGCGGT
GTTCGGCTACCTGAGCCTGTGGTCGGTGTTCTGGCTGTTCAAGCTGGTGACCGGCAAGGAGGGCATGGGCTACGGCGACT
TCAAGCTGCTGGCCATGCTCGGTGCCTGGGGCGGCTGGCAGATCCTGCCGCTGACCATCCTGCTGTCGTCGCTGGTCGGG
GCGATCCTCGGGGTGATCATGCTGCGCCTGCGCAACGCCGAAAGCGGCACGCCGATCCCCTTCGGTCCCTATCTGGCGAT
TGCCGGGTGGATTGCTTTGCTCTGGGGTGATCAAATAACCCGGACCTATCTGCAATTCGCCGGATTCAAATGA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (137-245)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-126)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	55.714	96.552	0.538
pilD	Vibrio campbellii strain DS40M4	53.004	97.586	0.517
pilD	Acinetobacter nosocomialis M2	50.53	97.586	0.493
pilD	Acinetobacter baumannii D1279779	50.53	97.586	0.493
pilD	Neisseria gonorrhoeae MS11	49.281	95.862	0.472