Detailed information NTG0431447

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: HB747_RS24550
• Genome accession: NZ_CP050322
• Coordinates: 5316257..5317129 (+)
• Length: 290 a.a.
• NCBI ID: WP_003107301.1
• Uniprot ID: -
• Organism: Pseudomonas aeruginosa strain DVT729
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5311257..5322129

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HB747_RS24535 (HB747_24570)	-	5312640..5313104 (-)	465	WP_021205141.1	pilin	-
HB747_RS24540 (HB747_24575)	pilB	5313335..5315035 (+)	1701	WP_021205142.1	type IV-A pilus assembly ATPase PilB	Machinery gene
HB747_RS24545 (HB747_24580)	pilC	5315039..5316256 (+)	1218	WP_003161763.1	type II secretion system F family protein	Machinery gene
HB747_RS24550 (HB747_24585)	pilD	5316257..5317129 (+)	873	WP_003107301.1	type IV prepilin peptidase/methyltransferase PilD	Machinery gene
HB747_RS24555 (HB747_24590)	coaE	5317126..5317737 (+)	612	WP_023114838.1	dephospho-CoA kinase	-
HB747_RS24560 (HB747_24595)	yacG	5317734..5317934 (+)	201	WP_003094656.1	DNA gyrase inhibitor YacG	-
HB747_RS24565 (HB747_24600)	-	5317971..5318180 (-)	210	WP_003094660.1	hypothetical protein	-
HB747_RS24570 (HB747_24605)	-	5318286..5318975 (-)	690	WP_003103868.1	energy-coupling factor ABC transporter permease	-
HB747_RS24575 (HB747_24610)	-	5318972..5319442 (-)	471	WP_019486073.1	hypothetical protein	-
HB747_RS24580 (HB747_24615)	-	5319439..5319864 (-)	426	WP_003103865.1	GNAT family N-acetyltransferase	-
HB747_RS24585 (HB747_24620)	-	5319997..5320626 (+)	630	WP_003120948.1	DUF1780 domain-containing protein	-
HB747_RS24590 (HB747_24625)	-	5320623..5321072 (+)	450	WP_003161439.1	MOSC domain-containing protein	-
HB747_RS24595 (HB747_24630)	-	5321098..5321268 (+)	171	WP_003094672.1	DUF3094 family protein	-

Sequence

Protein

• Length: 290 a.a.
• Molecular weight: 31873.90 Da
• Isoelectric Point: 7.7068

>NTDB_id=431447 HB747_RS24550 WP_003107301.1 5316257..5317129(+) (pilD) [Pseudomonas aeruginosa strain DVT729]
MTLLDYLASHPLAFVLCTILLGLLVGSFLNVVVHRLPKMMERNWKAEAREALGLEPEPKQATYNLVLPNSACPRCGHEIR
PWENIPLVSYLALGGKCSSCKAAIGKRYPLVELATALLSGYVAWHFGFTWQAGAMLLLTWGLLAMSLIDADHQLLPDVLV
LPLLWLGLIANHFGLFASLDDALFGAVFGYLSLWSVFWLFKLVTGKEGMGYGDFKLLAMLGAWGGWQILPLTILLSSLVG
AILGVIMLRLRNAESGTPIPFGPYLAIAGWIALLWGDQITRTYLQFAGFK

Nucleotide

• Length: 873 bp

>NTDB_id=431447 HB747_RS24550 WP_003107301.1 5316257..5317129(+) (pilD) [Pseudomonas aeruginosa strain DVT729]
ATGACCCTCCTCGACTACCTGGCCAGCCATCCGCTGGCCTTTGTCTTGTGCACCATCCTCCTCGGCCTGCTGGTCGGCAG
CTTCCTCAATGTCGTCGTGCATCGCCTGCCGAAGATGATGGAGCGCAACTGGAAGGCCGAGGCCCGCGAAGCGCTCGGGC
TGGAGCCGGAACCCAAGCAGGCGACCTACAACCTGGTGCTGCCGAATTCGGCGTGCCCGCGCTGCGGCCACGAGATCCGC
CCCTGGGAAAACATCCCGCTGGTCAGCTACCTGGCGCTGGGCGGCAAGTGCTCGTCCTGCAAGGCCGCCATCGGCAAACG
CTACCCCCTGGTGGAACTGGCCACCGCGCTGCTTTCCGGCTACGTCGCCTGGCATTTCGGCTTCACCTGGCAGGCGGGCG
CGATGCTCCTGCTGACCTGGGGTCTGCTGGCGATGAGCCTGATCGATGCCGATCACCAACTGCTGCCGGACGTGCTGGTG
CTGCCGCTGCTCTGGCTGGGCCTGATCGCCAACCATTTCGGCCTCTTCGCCAGCCTCGACGATGCGCTGTTCGGCGCGGT
GTTCGGCTACCTGAGCCTGTGGTCGGTGTTCTGGCTGTTCAAGCTGGTGACCGGCAAGGAGGGCATGGGCTACGGCGACT
TCAAGCTGCTGGCCATGCTCGGTGCCTGGGGCGGCTGGCAGATCCTGCCGCTGACCATCCTGCTGTCGTCGCTGGTCGGG
GCGATCCTCGGGGTGATCATGCTGCGCCTGCGCAACGCCGAAAGCGGCACGCCGATCCCCTTCGGTCCCTATCTGGCTAT
TGCCGGGTGGATTGCTTTGCTCTGGGGTGATCAAATAACCCGGACCTATCTGCAATTCGCCGGATTCAAATGA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (137-245)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-126)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	55.357	96.552	0.534
pilD	Vibrio campbellii strain DS40M4	52.65	97.586	0.514
pilD	Acinetobacter nosocomialis M2	50.53	97.586	0.493
pilD	Acinetobacter baumannii D1279779	50.53	97.586	0.493
pilD	Neisseria gonorrhoeae MS11	48.921	95.862	0.469