Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   HBK83_RS03740 Genome accession   NZ_CP050311
Coordinates   788145..789125 (+) Length   326 a.a.
NCBI ID   WP_017382924.1    Uniprot ID   -
Organism   Enterobacter hormaechei strain CM18-216     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 783145..794125
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HBK83_RS03700 (HBK83_03710) - 783229..783555 (+) 327 WP_003862421.1 YggL family protein -
  HBK83_RS03705 (HBK83_03715) - 783612..784328 (+) 717 WP_017382928.1 DUF2884 domain-containing protein -
  HBK83_RS03710 (HBK83_03720) - 784412..784750 (-) 339 WP_017382927.1 endonuclease domain-containing protein -
  HBK83_RS03715 (HBK83_03725) hemW 784807..785955 (-) 1149 WP_017382926.1 radical SAM family heme chaperone HemW -
  HBK83_RS03720 (HBK83_03730) - 785948..786541 (-) 594 WP_015571839.1 XTP/dITP diphosphatase -
  HBK83_RS03725 (HBK83_03735) yggU 786545..786841 (-) 297 WP_003860023.1 DUF167 family protein YggU -
  HBK83_RS03730 (HBK83_03740) - 786838..787404 (-) 567 WP_015571838.1 YggT family protein -
  HBK83_RS03735 (HBK83_03745) - 787426..788127 (-) 702 WP_017382925.1 YggS family pyridoxal phosphate-dependent enzyme -
  HBK83_RS03740 (HBK83_03750) pilT 788145..789125 (+) 981 WP_017382924.1 type IV pilus twitching motility protein PilT Machinery gene
  HBK83_RS03745 (HBK83_03755) ruvX 789136..789552 (-) 417 WP_006811925.1 Holliday junction resolvase RuvX -
  HBK83_RS03750 (HBK83_03760) - 789552..790112 (-) 561 WP_006811924.1 YqgE/AlgH family protein -
  HBK83_RS03755 (HBK83_03765) gshB 790187..791134 (-) 948 WP_015571835.1 glutathione synthase -
  HBK83_RS03760 (HBK83_03770) rsmE 791166..791897 (-) 732 WP_017382923.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  HBK83_RS03765 (HBK83_03775) endA 791949..792656 (-) 708 WP_003860034.1 deoxyribonuclease I -
  HBK83_RS03770 (HBK83_03780) - 792751..793248 (-) 498 WP_015571833.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35665.59 Da        Isoelectric Point: 6.0405

>NTDB_id=431357 HBK83_RS03740 WP_017382924.1 788145..789125(+) (pilT) [Enterobacter hormaechei strain CM18-216]
MDVEEIVALSVKHNVSDLHLCSDSPPRWRRSGRLEPAPFPPPDVEALLKAWLNDEQQGAWWANGQVDFAVTLADGQRLRG
SAFKHMHGVSIALRLLPLTCPQLSALGVPRAIPELLSNDNGLILVTGATGSGKSTTLAAMVDFLNHQTDGHILTLEDPVE
FMYQSERCLIQQREIGQHSPSFAEALRSALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAIERLVDT
FPAQEKDPVRNQLAGSLRAVLAQKLRQDVQGGRVALYELLVNTSAAANLIREGKTWQLPGIIQTGQQAGMQNFEQSLAER
RAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=431357 HBK83_RS03740 WP_017382924.1 788145..789125(+) (pilT) [Enterobacter hormaechei strain CM18-216]
ATGGATGTGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAGTGATTCGCCACCGCG
CTGGCGCAGGTCAGGCCGTCTTGAACCTGCGCCGTTTCCGCCCCCGGATGTGGAGGCATTATTAAAAGCGTGGCTCAACG
ATGAACAGCAGGGCGCATGGTGGGCAAACGGGCAGGTGGATTTTGCCGTTACCCTCGCAGACGGTCAGCGGCTGCGCGGC
AGTGCGTTTAAGCATATGCACGGCGTTTCGATTGCGCTGCGGCTGTTGCCGCTGACGTGCCCGCAGCTCTCTGCGTTAGG
TGTGCCGCGAGCGATCCCGGAACTGCTGTCTAATGACAATGGCCTGATTCTGGTCACCGGCGCCACCGGCAGCGGAAAAT
CGACCACCCTGGCCGCGATGGTCGATTTCCTCAATCATCAGACAGACGGGCATATTCTGACCCTGGAAGATCCGGTGGAG
TTTATGTACCAGAGCGAACGTTGCCTGATCCAGCAGCGGGAGATAGGCCAGCACAGCCCGTCCTTTGCCGAGGCGCTACG
CAGCGCGCTGCGTGAAGATCCGGATGTGATTCTGCTTGGTGAGCTGCGCGACAGCGAGACGATACGCCTGGCGCTGACGG
CGGCGGAAACCGGCCATCTGGTGTTAGCCACGCTGCATACGCGCGGCGCGGCGCAGGCGATTGAGCGCCTGGTCGACACC
TTTCCGGCGCAGGAGAAAGATCCGGTGCGTAATCAGCTGGCGGGAAGCCTGCGTGCGGTCCTGGCGCAGAAGCTGCGTCA
GGATGTCCAGGGCGGGCGCGTGGCGCTGTATGAGCTGCTGGTGAATACGTCGGCGGCGGCCAACCTGATCCGCGAAGGCA
AAACCTGGCAACTGCCGGGCATTATTCAAACGGGGCAGCAGGCGGGAATGCAGAACTTTGAGCAGAGTCTGGCGGAGCGA
CGGGCGCAGGGGCGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(72-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.459

100

0.506
  pilT Vibrio cholerae strain A1552

50.459

100

0.506
  pilT Legionella pneumophila strain Lp02

48.758

98.773

0.482
  pilT Legionella pneumophila strain ERS1305867

48.758

98.773

0.482
  pilT Neisseria meningitidis 8013

47.561

100

0.479
  pilT Pseudomonas stutzeri DSM 10701

47.706

100

0.479
  pilT Neisseria gonorrhoeae MS11

47.256

100

0.475
  pilT Acinetobacter baumannii strain A118

47.095

100

0.472
  pilT Acinetobacter baumannii D1279779

47.095

100

0.472
  pilT Acinetobacter baylyi ADP1

46.789

100

0.469
  pilT Pseudomonas aeruginosa PAK

46.789

100

0.469
  pilT Acinetobacter nosocomialis M2

47.5

98.16

0.466
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.653

99.08

0.433
  pilU Pseudomonas stutzeri DSM 10701

37.879

100

0.383
  pilU Vibrio cholerae strain A1552

38.199

98.773

0.377