Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   VEJY3_RS14255 Genome accession   NC_016613
Coordinates   3147195..3147770 (-) Length   191 a.a.
NCBI ID   WP_014233151.1    Uniprot ID   -
Organism   Vibrio sp. EJY3     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3142195..3152770
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VEJY3_RS14230 (VEJY3_14040) aroB 3142494..3143594 (-) 1101 WP_014233146.1 3-dehydroquinate synthase -
  VEJY3_RS14235 (VEJY3_14045) aroK 3143626..3144144 (-) 519 WP_014233147.1 shikimate kinase AroK -
  VEJY3_RS14240 (VEJY3_14050) pilQ 3144333..3146036 (-) 1704 WP_014233148.1 type IV pilus secretin PilQ Machinery gene
  VEJY3_RS14245 (VEJY3_14055) pilP 3146104..3146619 (-) 516 WP_014233149.1 pilus assembly protein PilP Machinery gene
  VEJY3_RS14250 (VEJY3_14060) pilO 3146609..3147202 (-) 594 WP_014233150.1 type 4a pilus biogenesis protein PilO Machinery gene
  VEJY3_RS14255 (VEJY3_14065) pilN 3147195..3147770 (-) 576 WP_014233151.1 PilN domain-containing protein Machinery gene
  VEJY3_RS14260 (VEJY3_14070) pilM 3147754..3148770 (-) 1017 WP_014233152.1 type IV pilus assembly protein PilM Machinery gene
  VEJY3_RS14265 (VEJY3_14075) - 3148914..3151481 (+) 2568 WP_014233153.1 penicillin-binding protein 1A -
  VEJY3_RS14270 (VEJY3_14080) oxyR 3151546..3152454 (-) 909 WP_014233154.1 DNA-binding transcriptional regulator OxyR -

Sequence

Protein


Download         Length: 191 a.a.        Molecular weight: 22362.75 Da        Isoelectric Point: 5.9580

>NTDB_id=43109 VEJY3_RS14255 WP_014233151.1 3147195..3147770(-) (pilN) [Vibrio sp. EJY3]
MLYRINLLPWRENQREEHRRRFVGMFILGLFCAVGIQWGIGKFYEIQQDQQQDRLDYLTHYITELDQRIEAMKIAEQEHS
KILERLKVVEGLQNGRNKTTEFMNLMPAVIPEGVYVDKIKMNDLEIEIAGISDSTPRLATMLDNMERSAKLLDVEMHSIV
HGKERFGKEFQTFKVSFMFKAVEPEEGGQHG

Nucleotide


Download         Length: 576 bp        

>NTDB_id=43109 VEJY3_RS14255 WP_014233151.1 3147195..3147770(-) (pilN) [Vibrio sp. EJY3]
GTGCTTTATCGAATTAACTTATTGCCCTGGAGGGAGAACCAAAGGGAAGAACATCGCCGTCGTTTTGTTGGCATGTTCAT
TCTGGGCTTGTTTTGTGCTGTTGGTATTCAATGGGGGATTGGGAAGTTTTATGAGATCCAACAAGATCAGCAGCAAGACC
GACTCGATTACCTTACTCACTATATTACGGAACTTGATCAACGTATTGAAGCAATGAAAATTGCCGAGCAAGAGCATAGC
AAAATATTAGAGCGTTTGAAGGTGGTTGAAGGTTTGCAAAATGGTCGCAACAAAACCACAGAGTTCATGAACCTGATGCC
TGCTGTTATTCCTGAAGGCGTTTACGTCGATAAGATCAAAATGAATGATCTGGAAATCGAAATCGCGGGGATTTCTGACA
GCACACCACGTCTGGCAACCATGCTCGATAACATGGAGCGTTCGGCAAAGCTATTGGACGTTGAAATGCACTCTATTGTC
CATGGCAAAGAACGCTTCGGTAAAGAATTTCAAACCTTCAAAGTGTCTTTCATGTTTAAAGCAGTAGAGCCGGAAGAGGG
AGGTCAGCATGGTTAA

Domains

Predicted by InterproScan.

Fimbrial assembly protein (PilN) (PilN)

(103-176)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Vibrio campbellii strain DS40M4

82.723

100

0.827
  pilN Vibrio cholerae strain A1552

59.896

100

0.602

Multiple sequence alignment