Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA/cflA   Type   Machinery gene
Locus tag   HA248_RS10960 Genome accession   NZ_CP050175
Coordinates   2111083..2112381 (-) Length   432 a.a.
NCBI ID   WP_000867590.1    Uniprot ID   A0AA95DCE6
Organism   Streptococcus pneumoniae strain PZ900701590     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2106083..2117381
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HA248_RS10940 (HA248_10915) rplI 2107238..2107690 (-) 453 WP_000864220.1 50S ribosomal protein L9 -
  HA248_RS10945 (HA248_10920) - 2107687..2109660 (-) 1974 WP_000715129.1 DHH family phosphoesterase -
  HA248_RS10950 (HA248_10925) raiA 2109796..2110344 (-) 549 WP_033705088.1 ribosome-associated translation inhibitor RaiA -
  HA248_RS10955 (HA248_10930) comFC/cflB 2110424..2111086 (-) 663 WP_000649971.1 ComF family protein Machinery gene
  HA248_RS10960 (HA248_10935) comFA/cflA 2111083..2112381 (-) 1299 WP_000867590.1 DEAD/DEAH box helicase Machinery gene
  HA248_RS10965 (HA248_10940) - 2112437..2113072 (+) 636 WP_000395739.1 YigZ family protein -
  HA248_RS10970 (HA248_10945) cysK 2113357..2114277 (+) 921 WP_000029860.1 cysteine synthase A -
  HA248_RS10975 (HA248_10950) - 2114352..2114881 (-) 530 Protein_2120 transposase -
  HA248_RS10980 (HA248_10955) - 2114863..2115171 (+) 309 Protein_2121 transposase -
  HA248_RS10985 (HA248_10960) tnpB 2115194..2115544 (-) 351 WP_033705086.1 IS66 family insertion sequence element accessory protein TnpB -
  HA248_RS10990 (HA248_10965) tsf 2115791..2116831 (-) 1041 WP_000808063.1 translation elongation factor Ts -

Sequence

Protein


Download         Length: 432 a.a.        Molecular weight: 49574.61 Da        Isoelectric Point: 8.5992

>NTDB_id=430582 HA248_RS10960 WP_000867590.1 2111083..2112381(-) (comFA/cflA) [Streptococcus pneumoniae strain PZ900701590]
MKVNLDYLGRLFTENELTEEERQLAEKLPAMRKEKGKLFCQRCNSIILEEWYLPIGAYYCRECLLMKRVRSDQTLYYFPQ
EDFPKQDVLKWRGQLTPFQEKVSEGLLQAVDKQKPTLVHAVTGAGKTEMIYQVVAKVINAGGAVCLASPRIDVCLELYKR
LQQDFSCGIALLHGESEPYFRTPLVVATTHQLLKFYQAFDLLIVDEVDAFPYVDNPMLYHAVKNSVKENGLRIFLTATST
NELDKKVRLGELKRLNLPRRFHGNPLIIPKPIWLSDFNRYLDKNRLSPKLKSYIEKQRKTAYPLLIFASEIKKGEQLAEI
LQEQFPNEKIGFVSSVTEDRLEQVQAFRDGELTILISTTILERGVTFPCVDVFVVEANHRLFTKSSLIQIGGRVGRSMDR
PTGDLLFFHDGLNASIKKAIKEIQMMNKEAGL

Nucleotide


Download         Length: 1299 bp        

>NTDB_id=430582 HA248_RS10960 WP_000867590.1 2111083..2112381(-) (comFA/cflA) [Streptococcus pneumoniae strain PZ900701590]
ATGAAAGTAAATTTAGATTATCTCGGTCGTTTATTTACTGAGAATGAATTAACAGAAGAAGAACGTCAGTTGGCGGAGAA
ACTTCCAGCAATGAGAAAGGAGAAGGGGAAACTTTTCTGTCAACGCTGTAATAGTATTATTCTAGAAGAATGGTATTTGC
CCATCGGTGCTTACTATTGTCGAGAGTGCTTGCTGATGAAGCGAGTCAGAAGTGATCAAACTTTATACTATTTTCCGCAG
GAGGATTTTCCGAAGCAAGATGTTCTCAAATGGCGCGGCCAATTAACTCCTTTTCAAGAGAAGGTGTCAGAGGGACTGCT
TCAAGCAGTAGACAAGCAAAAGCCAACCTTAGTTCATGCGGTAACAGGAGCTGGAAAGACAGAAATGATTTATCAAGTAG
TGGCTAAAGTGATCAATGCGGGTGGTGCAGTGTGTTTGGCTAGTCCTCGCATAGATGTTTGTTTGGAGCTGTACAAGCGC
CTGCAACAGGATTTTTCTTGCGGAATAGCTTTGCTACATGGAGAATCGGAACCTTATTTTCGAACACCACTAGTTGTTGC
AACAACCCATCAGTTATTGAAGTTTTATCAAGCTTTTGATTTGCTGATAGTGGATGAAGTAGATGCTTTTCCTTATGTTG
ATAATCCCATGCTTTACCACGCTGTCAAGAATAGTGTAAAGGAGAATGGATTGAGAATCTTTTTAACAGCGACTTCGACC
AATGAGTTAGATAAAAAGGTCCGTTTAGGAGAACTAAAAAGACTGAATTTACCGAGACGGTTTCATGGAAATCCGTTGAT
TATTCCAAAACCAATTTGGTTATCGGATTTTAATCGCTACTTAGACAAGAATCGTTTGTCACCAAAGTTAAAGTCCTATA
TTGAGAAGCAGAGAAAGACAGCTTATCCGTTACTCATTTTTGCTTCAGAAATTAAGAAAGGGGAGCAGTTAGCAGAAATC
TTACAGGAGCAATTTCCAAATGAGAAAATTGGCTTTGTATCTTCTGTAACAGAGGATCGATTAGAGCAAGTACAAGCTTT
TCGAGATGGAGAACTGACAATACTTATCAGTACGACAATCTTGGAGCGTGGAGTTACCTTCCCTTGTGTGGATGTTTTCG
TAGTAGAGGCCAATCATCGTTTGTTTACCAAGTCTAGTTTGATTCAGATTGGTGGACGAGTTGGACGAAGCATGGATAGA
CCGACAGGAGATTTGCTTTTCTTCCATGATGGGTTAAATGCTTCAATCAAGAAGGCGATTAAGGAAATTCAGATGATGAA
TAAGGAGGCTGGTCTATGA

Domains

Predicted by InterproScan.

Type III restriction enzyme, res subunit (ResIII)

(94-238)

Helicase conserved C-terminal domain (Helicase_C)

(293-396)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA/cflA Streptococcus pneumoniae Rx1

99.769

100

0.998
  comFA/cflA Streptococcus pneumoniae D39

99.769

100

0.998
  comFA/cflA Streptococcus pneumoniae R6

99.769

100

0.998
  comFA/cflA Streptococcus pneumoniae TIGR4

99.537

100

0.995
  comFA/cflA Streptococcus mitis NCTC 12261

93.287

100

0.933
  comFA/cflA Streptococcus mitis SK321

92.361

100

0.924
  comFA Lactococcus lactis subsp. cremoris KW2

52.696

94.444

0.498
  comFA Latilactobacillus sakei subsp. sakei 23K

38.979

99.769

0.389