Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   HBA20_RS25970 Genome accession   NZ_CP050147
Coordinates   5518057..5518929 (+) Length   290 a.a.
NCBI ID   WP_003125204.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain A5803     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5513057..5523929
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HBA20_RS25945 (HBA20_25990) nadC 5513388..5514236 (+) 849 WP_003094645.1 carboxylating nicotinate-nucleotide diphosphorylase -
  HBA20_RS25955 (HBA20_26000) pilA 5514448..5514897 (-) 450 WP_031627735.1 type 4a pilus biogenesis protein PilA Machinery gene
  HBA20_RS25960 (HBA20_26005) pilB 5515129..5516829 (+) 1701 WP_003112841.1 type IV-A pilus assembly ATPase PilB Machinery gene
  HBA20_RS25965 (HBA20_26010) pilC 5516833..5518053 (+) 1221 WP_009315898.1 type 4a pilus biogenesis protein PilC Machinery gene
  HBA20_RS25970 (HBA20_26015) pilD 5518057..5518929 (+) 873 WP_003125204.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  HBA20_RS25975 (HBA20_26020) coaE 5518926..5519537 (+) 612 WP_003094654.1 dephospho-CoA kinase -
  HBA20_RS25980 (HBA20_26025) yacG 5519534..5519734 (+) 201 WP_017148551.1 DNA gyrase inhibitor YacG -
  HBA20_RS25985 (HBA20_26030) - 5519774..5519980 (-) 207 Protein_5149 hypothetical protein -
  HBA20_RS25990 (HBA20_26035) - 5520086..5520775 (-) 690 WP_003103868.1 energy-coupling factor ABC transporter permease -
  HBA20_RS25995 (HBA20_26040) - 5520772..5521242 (-) 471 WP_023096122.1 hypothetical protein -
  HBA20_RS26000 (HBA20_26045) - 5521239..5521664 (-) 426 WP_003103865.1 GNAT family N-acetyltransferase -
  HBA20_RS26005 (HBA20_26050) - 5521797..5522426 (+) 630 WP_003094668.1 DUF1780 domain-containing protein -
  HBA20_RS26010 (HBA20_26055) - 5522423..5522872 (+) 450 WP_003094670.1 MOSC domain-containing protein -
  HBA20_RS26015 (HBA20_26060) - 5522898..5523068 (+) 171 WP_003094672.1 DUF3094 family protein -

Sequence

Protein


Download         Length: 290 a.a.        Molecular weight: 31839.89 Da        Isoelectric Point: 7.7068

>NTDB_id=430349 HBA20_RS25970 WP_003125204.1 5518057..5518929(+) (pilD) [Pseudomonas aeruginosa strain A5803]
MPLLDYLASHPLAFVLCAILLGLLVGSFLNVVVHRLPKMMERNWKAEAREALGLEPEPKQATYNLVLPNSACPRCGHEIR
PWENIPLVSYLALGGKCSSCKAAIGKRYPLVELATALLSGYVAWHFGFTWQAGAMLLLTWGLLAMSLIDADHQLLPDVLV
LPLLWLGLIANHFGLFASLDDALFGAVFGYLSLWSVFWLFKLVTGKEGMGYGDFKLLAMLGAWGGWQILPLTILLSSLVG
AILGVIMLRLRNAESGTPIPFGPYLAIAGWIALLWGDQITRTYLQFAGFK

Nucleotide


Download         Length: 873 bp        

>NTDB_id=430349 HBA20_RS25970 WP_003125204.1 5518057..5518929(+) (pilD) [Pseudomonas aeruginosa strain A5803]
ATGCCCCTCCTCGACTACCTGGCCAGCCATCCGCTGGCCTTTGTCTTGTGCGCCATCCTCCTTGGCCTGCTGGTCGGCAG
CTTCCTCAACGTCGTCGTGCATCGCCTGCCGAAGATGATGGAGCGCAACTGGAAGGCCGAAGCCCGCGAAGCGCTCGGGC
TGGAGCCGGAACCCAAGCAGGCGACCTACAACCTGGTGCTGCCGAATTCGGCGTGCCCGCGCTGCGGCCACGAGATCCGC
CCCTGGGAAAACATCCCGCTGGTCAGCTACCTGGCGCTGGGCGGCAAGTGCTCGTCCTGCAAGGCCGCCATCGGCAAACG
CTACCCCCTGGTGGAACTGGCCACCGCGCTGCTTTCCGGCTACGTCGCCTGGCATTTCGGCTTCACCTGGCAGGCGGGCG
CGATGCTCCTGCTGACCTGGGGTCTGCTGGCGATGAGCCTGATCGATGCCGATCACCAACTGCTGCCGGACGTGCTGGTG
CTGCCGCTGCTCTGGCTGGGCCTGATCGCCAACCATTTCGGCCTCTTCGCCAGCCTCGACGATGCGCTGTTCGGCGCGGT
GTTCGGCTACCTGAGCCTGTGGTCGGTGTTCTGGCTGTTCAAGCTGGTGACCGGCAAGGAGGGCATGGGCTACGGCGACT
TCAAGCTGCTGGCCATGCTCGGTGCCTGGGGCGGCTGGCAGATCCTGCCGCTGACCATCCTGCTGTCGTCGCTGGTCGGG
GCGATCCTCGGGGTGATCATGCTGCGCCTGCGCAACGCCGAAAGCGGCACGCCGATCCCCTTCGGTCCCTATCTGGCGAT
TGCCGGGTGGATTGCTTTGCTCTGGGGTGATCAAATAACCCGGACCTATCTGCAATTCGCCGGATTCAAATGA

Domains

Predicted by InterproScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(20-126)

Type IV leader peptidase family (Peptidase_A24)

(137-245)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

55.714

96.552

0.538
  pilD Vibrio campbellii strain DS40M4

53.004

97.586

0.517
  pilD Acinetobacter nosocomialis M2

50.53

97.586

0.493
  pilD Acinetobacter baumannii D1279779

50.53

97.586

0.493
  pilD Neisseria gonorrhoeae MS11

49.281

95.862

0.472