Detailed information NTG0429090

Bioinformatically predicted

Overview

• Name: addA
• Type: Machinery gene
• Locus tag: BHV55_RS06050
• Genome accession: NZ_CP049978
• Coordinates: 1174184..1177909 (+)
• Length: 1241 a.a.
• NCBI ID: WP_070757220.1
• Uniprot ID: -
• Organism: Bacillus sp. RZ2MS9
• Function: homologous recombination; plasmid transformation (predicted from homology) Homologous recombination

Genomic Context

Location: 1169184..1182909

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BHV55_RS06035 (BHV55_06035)	-	1169342..1169935 (+)	594	WP_000347518.1	TVP38/TMEM64 family protein	-
BHV55_RS06040 (BHV55_06040)	lepB	1169992..1170555 (+)	564	WP_000751910.1	signal peptidase I	-
BHV55_RS06045 (BHV55_06045)	addB	1170672..1174187 (+)	3516	WP_000058607.1	helicase-exonuclease AddAB subunit AddB	Machinery gene
BHV55_RS06050 (BHV55_06050)	addA	1174184..1177909 (+)	3726	WP_070757220.1	helicase-exonuclease AddAB subunit AddA	Machinery gene
BHV55_RS06055 (BHV55_06055)	-	1177922..1178209 (+)	288	WP_000255725.1	RNA polymerase alpha subunit C-terminal domain-containing protein	-
BHV55_RS06060 (BHV55_06060)	gerPF	1178347..1178562 (-)	216	WP_001141566.1	spore germination protein GerPF	-
BHV55_RS06065 (BHV55_06065)	-	1178605..1178991 (-)	387	WP_000902319.1	spore germination protein GerPE	-
BHV55_RS06070 (BHV55_06070)	gerPD	1179007..1179201 (-)	195	WP_001052807.1	spore germination protein GerPD	-
BHV55_RS06075 (BHV55_06075)	gerPC	1179208..1179822 (-)	615	WP_001070760.1	spore germination protein GerPC	-
BHV55_RS06080 (BHV55_06080)	gerPB	1179890..1180096 (-)	207	WP_001012508.1	spore germination protein GerPB	-
BHV55_RS06085 (BHV55_06085)	gerPA	1180111..1180332 (-)	222	WP_001111187.1	spore germination protein GerPA	-
BHV55_RS06090 (BHV55_06090)	-	1180429..1180608 (-)	180	WP_000462845.1	aspartyl-phosphate phosphatase Spo0E family protein	-
BHV55_RS06095 (BHV55_06095)	-	1180855..1181754 (+)	900	WP_001213086.1	fumarylacetoacetate hydrolase family protein	-
BHV55_RS06100 (BHV55_06100)	-	1181790..1182074 (-)	285	WP_000926864.1	hypothetical protein	-

Sequence

Protein

• Length: 1241 a.a.
• Molecular weight: 142643.71 Da
• Isoelectric Point: 4.7840

>NTDB_id=429090 BHV55_RS06050 WP_070757220.1 1174184..1177909(+) (addA) [Bacillus sp. RZ2MS9]
MIENWPEKPEGSQWTDDQWKAVVANGRDILVAAAAGSGKTAVLVERIIKKIINEENPVDVDRLLVVTFTNAAAQEMKNRI
GEALEKVLIDEPGSQHVRKQLSLLNKASISTIHSFCLQVIRGYYYMLDVDPRFRIANQTENELLKEEVLDDILEEEYGIE
DNTIFFELVDRYTSDRSDDDLQRMILALHTESRAHPNPEKWLDKLVEAYDVEGKTIEDLVYASYLLEDVKFQLETAEQHI
RKATELAMLPDGPAPRIETLQADLALLGTLSSAAHESWTSLYEAMQNVSWQTLKRIKKSDYNEDVVKQVDSLRNKAKDEV
KKLQEELFSRKPESFLRDFQDMHPVLEKLVQLVKVFTERFQAMKRDKGMVDFTDLEHFCLQILSEQSENGEMNPSAVALQ
YRNKFAEVLVDEYQDTNFVQESIIKFVTKDSESEGNLFMVGDVKQSIYRFRLAEPGLFLGKYKRFTQEGLGGGMKIDLAK
NFRSRHEVLAGTNFIFKQIMGEEVGEIDYDADAELKLGATYPEGEDVAAELLCIQQTEEEVIDGEEGAEVEKAQLEARLM
AQRIKAMVDSGYEVYDRKNDSMRPVQYRDFVILLRSMPWAPQIMEELKLQGIPVYADLATGYFEATEVNIMMNVFRVIDN
PMQDIPLAAVLRSPIVGLSDEELATLRAHGKKSSFYEVMSSFLKGAPLEEEQELHDKLEWFYNLLQGWREFARQQSLSDL
IWKVYGETGYYDFVGGLPAGKQRQANLRVLYDRARQYEATSFRGLFRFLRFIERILERGDDMGTARALGEQEDVVRIMTI
HKSKGLEFPVVFVAGLGRRFNTQDLMKRFLLHKDFGFGSQFIDPRKRIKYTTLSQLAIKRKMKMELIAEEMRVLYVALTR
AKEKLILIGTVKDANKEMEKWLDAREHSEWLLPDHIRAGASCYLDWIAPSLYRHRDSEMLLELGQGSIPDKIYGYDTSWK
VEVVDGNTLLAPEPVQEEKQELLEALREKKAVPLESERKDEVYDRLMWKYGYEEATSHRAKQSVTEIKRNYQSEDGSDNA
FIKKLRAPIKTRPRFMEKKGLTYAERGTAVHAVMQHVDLKKPITVEVLQEQIAGMVNKELLTFEQAEEIAIEKVISFFDS
DLGKRVLAAKSVEREVPFTMMLAAEEAYQDWQGNSGESILVQGVIDCMIEEEDGITLIDFKTDTIEGKFPGGFEQAKPIL
EDRYKVQLSLYAKALEKSLQHPVKEKCLYFFDGNHVVNIEE

Nucleotide

• Length: 3726 bp

>NTDB_id=429090 BHV55_RS06050 WP_070757220.1 1174184..1177909(+) (addA) [Bacillus sp. RZ2MS9]
ATGATAGAAAATTGGCCTGAAAAACCAGAAGGTAGTCAATGGACAGATGACCAGTGGAAAGCTGTTGTAGCGAATGGACG
TGATATTTTAGTTGCGGCAGCAGCTGGGTCAGGGAAAACAGCAGTATTAGTTGAACGTATTATTAAAAAGATTATTAATG
AAGAGAATCCAGTCGATGTCGACCGCCTGCTCGTTGTAACATTTACGAATGCAGCGGCGCAAGAGATGAAAAACCGAATC
GGGGAAGCGTTAGAAAAGGTATTAATTGATGAACCAGGCTCTCAACACGTAAGAAAGCAGCTGAGCCTATTAAATAAAGC
TTCCATTTCAACAATTCACTCATTCTGTTTACAAGTAATTAGAGGATATTACTACATGCTTGATGTTGATCCTCGTTTCC
GTATTGCTAATCAAACAGAAAATGAATTGTTAAAAGAAGAAGTGCTAGATGACATATTAGAAGAAGAGTACGGAATCGAA
GATAATACAATCTTCTTTGAACTTGTTGATCGTTATACGAGTGACCGTAGTGATGATGACTTACAACGTATGATTTTAGC
GCTTCATACAGAGTCAAGAGCGCATCCAAATCCGGAAAAATGGCTTGATAAATTAGTAGAAGCATATGACGTAGAAGGAA
AGACAATTGAAGATTTAGTATATGCTTCTTATTTATTAGAAGATGTGAAATTCCAGCTTGAAACAGCGGAACAGCATATT
CGTAAGGCAACTGAACTCGCAATGCTTCCTGACGGTCCAGCGCCTCGCATTGAAACCCTGCAAGCAGATCTAGCTTTACT
TGGAACGCTATCATCAGCTGCTCATGAGTCGTGGACAAGTTTGTATGAAGCGATGCAAAATGTATCGTGGCAAACGTTAA
AGCGTATTAAGAAAAGTGATTACAATGAAGATGTTGTAAAACAAGTAGACTCTCTTCGTAATAAAGCGAAAGATGAAGTG
AAGAAATTACAAGAAGAGTTATTTAGCCGAAAACCTGAAAGTTTCTTACGAGATTTTCAAGATATGCACCCAGTATTAGA
AAAGCTTGTTCAACTTGTAAAAGTATTTACAGAGCGTTTCCAAGCGATGAAGCGAGATAAAGGAATGGTCGATTTTACAG
ATTTAGAGCATTTTTGTTTACAAATTTTAAGTGAACAAAGTGAAAATGGTGAAATGAATCCGTCAGCAGTGGCGCTCCAA
TATCGTAATAAATTTGCTGAAGTATTAGTCGATGAATATCAAGATACGAACTTTGTACAGGAATCTATTATTAAATTCGT
AACGAAAGATTCTGAGAGTGAAGGAAACTTGTTCATGGTTGGTGACGTAAAGCAGTCGATTTATCGTTTCCGACTAGCCG
AACCAGGACTATTCCTAGGAAAATATAAACGCTTCACACAAGAAGGATTGGGCGGTGGAATGAAGATCGACTTAGCGAAA
AACTTCCGTAGTCGTCATGAAGTGTTAGCAGGTACGAACTTTATCTTCAAACAAATTATGGGTGAAGAAGTCGGAGAAAT
CGACTACGATGCAGACGCTGAATTAAAGTTAGGTGCTACCTATCCAGAAGGTGAAGATGTAGCGGCAGAACTACTGTGCA
TTCAGCAAACGGAAGAAGAAGTAATAGACGGAGAAGAAGGTGCAGAAGTCGAAAAAGCACAGCTTGAAGCTCGTCTTATG
GCGCAGCGCATTAAAGCGATGGTTGATTCAGGTTATGAAGTGTATGACCGTAAAAATGATAGTATGCGCCCAGTACAATA
CCGCGACTTCGTTATTTTACTTCGCTCCATGCCGTGGGCCCCGCAAATTATGGAAGAGTTAAAATTACAAGGAATTCCAG
TGTACGCTGACCTTGCGACTGGTTACTTTGAAGCGACGGAAGTAAATATTATGATGAACGTATTCCGCGTTATTGATAAT
CCGATGCAAGATATTCCGCTTGCAGCTGTACTTCGTTCTCCAATCGTTGGATTGAGCGATGAAGAACTTGCAACGCTTCG
TGCTCATGGAAAGAAAAGCTCGTTTTATGAAGTAATGAGCTCATTCTTAAAAGGGGCACCACTTGAAGAGGAGCAAGAAC
TTCATGATAAATTAGAGTGGTTTTATAACTTACTGCAAGGATGGCGTGAATTTGCACGTCAACAATCACTTTCTGATTTA
ATTTGGAAAGTGTACGGTGAGACAGGTTATTACGACTTCGTTGGCGGTTTACCAGCTGGAAAGCAAAGGCAGGCAAACTT
ACGCGTACTATATGACCGCGCAAGACAATACGAAGCAACATCATTTAGAGGATTATTCCGCTTCTTACGTTTTATTGAAC
GTATTTTAGAACGCGGTGATGACATGGGTACGGCGAGGGCCCTTGGTGAACAAGAAGACGTTGTTCGCATTATGACGATT
CATAAAAGTAAAGGGTTAGAGTTCCCGGTTGTATTTGTAGCTGGACTCGGTCGTCGTTTTAATACACAAGACTTAATGAA
ACGTTTCTTATTGCATAAAGATTTCGGTTTCGGTTCGCAATTTATCGATCCGCGTAAACGAATTAAATATACAACATTAT
CACAGCTTGCGATTAAGCGTAAAATGAAAATGGAATTAATTGCGGAAGAAATGCGCGTACTATACGTAGCGTTGACGCGC
GCGAAAGAGAAGTTAATTTTAATCGGAACAGTTAAGGATGCAAATAAGGAAATGGAAAAATGGCTTGATGCGAGGGAACA
TAGTGAATGGTTATTACCAGATCACATACGTGCCGGGGCGTCTTGTTATTTAGACTGGATTGCACCTTCATTATATAGAC
ACCGTGATAGTGAAATGCTTCTTGAATTAGGACAAGGAAGTATTCCAGATAAAATTTACGGGTATGACACTAGCTGGAAA
GTAGAAGTTGTGGACGGTAATACGCTACTTGCACCAGAGCCAGTTCAAGAAGAGAAACAAGAATTGTTAGAAGCGCTTCG
TGAGAAAAAGGCTGTGCCGTTAGAAAGTGAACGAAAAGATGAAGTGTACGACAGATTAATGTGGAAGTACGGATATGAGG
AAGCAACATCTCACCGTGCGAAGCAATCTGTTACAGAAATAAAGAGAAATTACCAATCAGAAGATGGTAGTGATAACGCC
TTTATTAAAAAACTACGTGCACCAATTAAAACACGTCCGCGCTTTATGGAGAAAAAAGGGTTAACGTACGCAGAGCGCGG
AACAGCAGTCCATGCCGTTATGCAGCATGTTGATTTGAAGAAGCCGATTACGGTTGAAGTTCTTCAAGAGCAAATTGCTG
GAATGGTAAATAAGGAATTATTAACATTCGAGCAGGCGGAAGAAATAGCGATTGAAAAAGTAATTTCATTCTTTGACAGT
GACTTAGGTAAAAGGGTATTAGCGGCGAAAAGTGTTGAGCGTGAAGTTCCATTTACGATGATGCTTGCAGCAGAAGAAGC
ATATCAAGATTGGCAAGGGAATAGCGGGGAATCGATTCTTGTCCAAGGGGTTATCGACTGCATGATTGAAGAGGAAGATG
GCATTACGTTAATCGACTTCAAAACAGATACGATTGAAGGGAAATTTCCAGGCGGATTCGAACAAGCGAAACCAATTTTA
GAAGATCGATATAAAGTGCAGCTTTCGTTATATGCAAAAGCACTGGAGAAAAGCTTACAACATCCTGTAAAAGAGAAATG
CTTATACTTCTTTGATGGGAATCACGTTGTAAATATTGAAGAATAG

Domains

Predicted by InterproScan.

• UvrD-like helicase C-terminal domain (UvrD_C) (476-890)

• PD-(D/E)XK nuclease superfamily (PDDEXK_1) (1060-1231)

• UvrD/REP helicase N-terminal domain (UvrD-helicase) (14-465)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
addA	Bacillus subtilis subsp. subtilis str. 168	53.488	100	0.537