Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   G4G26_RS12315 Genome accession   NZ_CP049924
Coordinates   2423136..2424206 (-) Length   356 a.a.
NCBI ID   WP_015714258.1    Uniprot ID   -
Organism   Bacillus subtilis strain So1b     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2418136..2429206
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G4G26_RS12270 (G4G26_12305) tapA 2418615..2419376 (-) 762 WP_015714251.1 amyloid fiber anchoring/assembly protein TapA -
  G4G26_RS12275 (G4G26_12310) yqzG 2419648..2419974 (+) 327 WP_003246051.1 YqzG/YhdC family protein -
  G4G26_RS12280 (G4G26_12315) spoIIT 2420016..2420195 (-) 180 WP_014480252.1 YqzE family protein -
  G4G26_RS12285 (G4G26_12320) comGG 2420266..2420640 (-) 375 WP_015714252.1 ComG operon protein ComGG Machinery gene
  G4G26_RS12290 (G4G26_12325) comGF 2420641..2421024 (-) 384 WP_038429292.1 ComG operon protein ComGF Machinery gene
  G4G26_RS12295 (G4G26_12330) comGE 2421050..2421397 (-) 348 WP_015714254.1 ComG operon protein 5 Machinery gene
  G4G26_RS12300 (G4G26_12335) comGD 2421381..2421812 (-) 432 WP_015714255.1 comG operon protein ComGD Machinery gene
  G4G26_RS12305 (G4G26_12340) comGC 2421802..2422098 (-) 297 WP_003230162.1 comG operon protein ComGC Machinery gene
  G4G26_RS12310 (G4G26_12345) comGB 2422112..2423149 (-) 1038 WP_015714257.1 comG operon protein ComGB Machinery gene
  G4G26_RS12315 (G4G26_12350) comGA 2423136..2424206 (-) 1071 WP_015714258.1 competence protein ComGA Machinery gene
  G4G26_RS12320 (G4G26_12355) - 2424418..2424615 (-) 198 WP_014480259.1 CBS domain-containing protein -
  G4G26_RS12325 (G4G26_12360) corA 2424617..2425570 (-) 954 WP_004399136.1 magnesium transporter CorA -
  G4G26_RS12330 (G4G26_12365) yqhB 2425713..2427041 (+) 1329 WP_074794463.1 hemolysin family protein -
  G4G26_RS12335 (G4G26_12370) rsbRD 2427094..2427930 (-) 837 WP_015714260.1 RsbT co-antagonist protein RsbRD -
  G4G26_RS12345 (G4G26_12380) mgsR 2428154..2428534 (-) 381 WP_003230147.1 transcriptional regulator MgsR -
  G4G26_RS12350 (G4G26_12385) yqgY 2428766..2429011 (+) 246 WP_003226300.1 DUF2626 domain-containing protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40488.67 Da        Isoelectric Point: 9.0175

>NTDB_id=428741 G4G26_RS12315 WP_015714258.1 2423136..2424206(-) (comGA) [Bacillus subtilis strain So1b]
MDSIEKVSKNLIEEAYLTKASDIHIVPRERDAIIHFRVDHALLKKRDMKKEECVRLISHFKFLSAMDIGERRKPQNGSLT
LKLKEGNVHLRMSTLPTINEESLVIRVMPQYNIPSIDKLSLFPKTGATLLSFLKHSHGMLIFTGPTGSGKTTTLYSLVQY
AKKHFNRNIVTLEDPVETRDEDVLQVQVNEKAGVTYSTGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLTSLH
TRDAKGAIYRLLEFGINMNEIEQTVIAIAAQRLVDLACPFCENGCSSVYCRQSRNTRRASVYELLYGKNLQQCIQEAKGN
HANYQYQTLRQIIRKGIALGYLTTNNYDRWVYHEKD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=428741 G4G26_RS12315 WP_015714258.1 2423136..2424206(-) (comGA) [Bacillus subtilis strain So1b]
TTGGATTCAATAGAAAAGGTAAGCAAAAACTTGATTGAAGAAGCATATCTAACAAAGGCTTCTGATATTCACATTGTGCC
GAGGGAGCGGGACGCTATCATTCATTTTCGGGTCGATCATGCCTTGCTGAAAAAAAGGGACATGAAAAAAGAAGAGTGCG
TAAGGCTGATTTCACATTTTAAATTTCTTTCAGCAATGGATATAGGTGAAAGGCGAAAGCCGCAAAACGGTTCGCTTACG
TTAAAGCTGAAAGAGGGAAATGTTCATTTAAGAATGTCAACGCTGCCCACAATTAATGAAGAAAGCCTCGTGATCAGAGT
GATGCCCCAATACAATATCCCTTCGATTGATAAATTGTCGCTATTTCCGAAGACAGGAGCCACATTACTCTCGTTTTTAA
AACATTCCCATGGCATGCTCATTTTTACCGGGCCGACTGGTTCAGGGAAGACTACCACATTATACTCTCTCGTTCAATAT
GCAAAAAAACACTTTAATCGAAATATTGTCACATTAGAGGACCCTGTTGAAACAAGGGACGAAGATGTTCTTCAGGTTCA
GGTGAATGAAAAAGCCGGTGTAACTTATTCCACAGGTCTGAAAGCAATTTTGCGCCATGATCCCGATATGATTATTTTAG
GTGAGATCAGAGACGCGGAAACAGCTGAAATTGCGGTGCGGGCAGCGATGACGGGACATCTGGTACTGACGAGCCTTCAT
ACGAGAGACGCAAAGGGCGCAATTTACAGACTGCTTGAATTCGGTATCAATATGAATGAAATCGAACAGACTGTCATTGC
AATAGCGGCTCAGCGCTTGGTTGATTTGGCTTGCCCGTTTTGTGAAAACGGATGTTCATCAGTGTATTGCCGACAGTCAC
GAAATACCAGGAGAGCTAGCGTTTATGAGCTTCTATACGGGAAAAATCTTCAGCAATGTATCCAGGAGGCAAAAGGAAAT
CATGCAAATTACCAATATCAAACGCTTCGTCAAATTATCAGAAAAGGAATTGCACTCGGCTATTTAACGACAAACAACTA
TGACCGGTGGGTTTATCATGAAAAAGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

99.719

100

0.997