Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   G4G26_RS12310 Genome accession   NZ_CP049924
Coordinates   2422112..2423149 (-) Length   345 a.a.
NCBI ID   WP_015714257.1    Uniprot ID   -
Organism   Bacillus subtilis strain So1b     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2417112..2428149
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G4G26_RS12260 (G4G26_12295) tasA 2417210..2417995 (-) 786 WP_014664586.1 biofilm matrix protein TasA -
  G4G26_RS12265 (G4G26_12300) sipW 2418059..2418631 (-) 573 WP_003230181.1 signal peptidase I -
  G4G26_RS12270 (G4G26_12305) tapA 2418615..2419376 (-) 762 WP_015714251.1 amyloid fiber anchoring/assembly protein TapA -
  G4G26_RS12275 (G4G26_12310) yqzG 2419648..2419974 (+) 327 WP_003246051.1 YqzG/YhdC family protein -
  G4G26_RS12280 (G4G26_12315) spoIIT 2420016..2420195 (-) 180 WP_014480252.1 YqzE family protein -
  G4G26_RS12285 (G4G26_12320) comGG 2420266..2420640 (-) 375 WP_015714252.1 ComG operon protein ComGG Machinery gene
  G4G26_RS12290 (G4G26_12325) comGF 2420641..2421024 (-) 384 WP_038429292.1 ComG operon protein ComGF Machinery gene
  G4G26_RS12295 (G4G26_12330) comGE 2421050..2421397 (-) 348 WP_015714254.1 ComG operon protein 5 Machinery gene
  G4G26_RS12300 (G4G26_12335) comGD 2421381..2421812 (-) 432 WP_015714255.1 comG operon protein ComGD Machinery gene
  G4G26_RS12305 (G4G26_12340) comGC 2421802..2422098 (-) 297 WP_003230162.1 comG operon protein ComGC Machinery gene
  G4G26_RS12310 (G4G26_12345) comGB 2422112..2423149 (-) 1038 WP_015714257.1 comG operon protein ComGB Machinery gene
  G4G26_RS12315 (G4G26_12350) comGA 2423136..2424206 (-) 1071 WP_015714258.1 competence protein ComGA Machinery gene
  G4G26_RS12320 (G4G26_12355) - 2424418..2424615 (-) 198 WP_014480259.1 CBS domain-containing protein -
  G4G26_RS12325 (G4G26_12360) corA 2424617..2425570 (-) 954 WP_004399136.1 magnesium transporter CorA -
  G4G26_RS12330 (G4G26_12365) yqhB 2425713..2427041 (+) 1329 WP_074794463.1 hemolysin family protein -
  G4G26_RS12335 (G4G26_12370) rsbRD 2427094..2427930 (-) 837 WP_015714260.1 RsbT co-antagonist protein RsbRD -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 39733.22 Da        Isoelectric Point: 9.7900

>NTDB_id=428740 G4G26_RS12310 WP_015714257.1 2422112..2423149(-) (comGB) [Bacillus subtilis strain So1b]
MKKIRKVWSLKDQARLLKRLGEMTAGGYTLLDGLRLMELQMNKRQAADLTDTVTCLREGAPFYQVLKSLSFHKEAVGICY
FAETHGELPASMIQSGELLERKIAQADQLKRVLRYPLFLIFTVAVMFYMLQSIIIPQFSGIYQSMNMETSRSTDMLFAFF
QHIDLVIILLVLFTAGIGIYYWLVFKKKSPARQMLICIRIPLVGKLVKLFNSYFFSLQLSSLLKSGLSIYDSLNAFKHQT
FLPFYRCEAEQLIERLKAGKSIESAICGSPFYETDLSKVISHGQLSGRLDRELFTYSQFILQRLEHKAQKWTGILQPMIY
GFVAAMILLVYLSMLVPMYQMMNQM

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=428740 G4G26_RS12310 WP_015714257.1 2422112..2423149(-) (comGB) [Bacillus subtilis strain So1b]
ATGAAAAAGATTAGAAAAGTCTGGTCGTTAAAGGATCAAGCCAGGTTATTAAAGAGGCTCGGTGAAATGACTGCGGGCGG
ATATACGCTTCTGGATGGATTACGCCTGATGGAACTTCAGATGAATAAGAGGCAGGCGGCTGACTTGACTGATACGGTCA
CTTGTTTGAGGGAAGGGGCTCCGTTTTATCAAGTACTAAAGAGTTTGTCATTTCATAAGGAAGCCGTAGGTATTTGTTAT
TTTGCTGAAACACATGGTGAACTGCCTGCTTCAATGATCCAGAGCGGAGAGCTGCTGGAACGAAAAATTGCACAGGCAGA
CCAGCTGAAAAGAGTGCTGCGCTATCCGCTTTTCCTCATCTTTACGGTCGCTGTCATGTTTTATATGTTACAGTCCATCA
TCATTCCTCAGTTTTCCGGTATCTATCAATCGATGAATATGGAAACTTCACGTTCAACCGATATGCTTTTTGCTTTTTTT
CAGCATATTGATCTTGTGATCATTTTGCTTGTTCTTTTTACAGCAGGTATCGGGATTTATTATTGGCTTGTGTTTAAGAA
AAAATCACCTGCCCGGCAAATGCTGATTTGTATCAGGATTCCTTTGGTTGGAAAGCTTGTCAAGCTGTTTAACAGCTACT
TTTTTTCTTTGCAGCTAAGCAGCCTTTTAAAATCAGGCCTCTCAATTTATGACAGCCTTAATGCATTTAAACATCAAACG
TTTCTCCCTTTCTACCGCTGCGAGGCTGAACAATTGATTGAACGGCTAAAAGCCGGTAAGTCAATTGAATCCGCTATTTG
TGGAAGCCCTTTTTATGAAACTGATTTATCAAAAGTCATATCTCACGGCCAGCTGAGCGGCCGATTGGATCGGGAGCTTT
TCACATACAGCCAATTCATATTACAGCGGCTGGAACACAAAGCGCAAAAATGGACAGGCATCCTTCAGCCAATGATTTAT
GGATTTGTTGCAGCGATGATCTTACTTGTGTATTTATCTATGCTTGTGCCTATGTATCAGATGATGAATCAAATGTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(17-136)

Type II secretion system (T2SS), protein F (T2SSF)

(215-337)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Bacillus subtilis subsp. subtilis str. 168

99.071

93.623

0.928