Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   G8A07_RS25870 Genome accession   NZ_CP049919
Coordinates   5618178..5619686 (+) Length   502 a.a.
NCBI ID   WP_195794776.1    Uniprot ID   A0A7S9CBN1
Organism   Roseateles sp. DAIF2     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5613178..5624686
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G8A07_RS25845 (G8A07_25855) argS 5613579..5615309 (+) 1731 WP_195794771.1 arginine--tRNA ligase -
  G8A07_RS25850 (G8A07_25860) - 5615327..5615965 (+) 639 WP_195794772.1 SPOR domain-containing protein -
  G8A07_RS25855 (G8A07_25865) - 5616022..5616690 (+) 669 WP_195794773.1 thiol:disulfide interchange protein DsbA/DsbL -
  G8A07_RS25860 (G8A07_25870) lptA 5616752..5617393 (+) 642 WP_195794774.1 lipopolysaccharide transport periplasmic protein LptA -
  G8A07_RS25865 (G8A07_25875) lptB 5617390..5618163 (+) 774 WP_195794775.1 LPS export ABC transporter ATP-binding protein -
  G8A07_RS25870 (G8A07_25880) rpoN/rpoN1 5618178..5619686 (+) 1509 WP_195794776.1 RNA polymerase factor sigma-54 Machinery gene
  G8A07_RS25875 (G8A07_25885) - 5619736..5621001 (+) 1266 WP_195794777.1 class I SAM-dependent RNA methyltransferase -
  G8A07_RS25880 (G8A07_25890) - 5621037..5621537 (-) 501 WP_195794778.1 hypothetical protein -
  G8A07_RS25885 (G8A07_25895) - 5621583..5621960 (-) 378 WP_195794779.1 ankyrin repeat domain-containing protein -
  G8A07_RS25890 (G8A07_25900) - 5621964..5622692 (-) 729 WP_195794780.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  G8A07_RS25895 (G8A07_25905) - 5622805..5623212 (+) 408 WP_195794781.1 GFA family protein -
  G8A07_RS25900 (G8A07_25910) - 5623229..5623714 (-) 486 WP_195794782.1 hypothetical protein -

Sequence

Protein


Download         Length: 502 a.a.        Molecular weight: 55401.62 Da        Isoelectric Point: 4.5733

>NTDB_id=428692 G8A07_RS25870 WP_195794776.1 5618178..5619686(+) (rpoN/rpoN1) [Roseateles sp. DAIF2]
MKPSLQVRLSQHLALTPQLQQSIRLLQLSTLELNQEVEQMLAQNPLLETDEEFADQPAPEMPEPRSSASNDEPSGQAADA
EAPSEEMRAEDFGTTEREDWENGTERDDFDGIRELPGSGASGNGDEDGERPEQEAPSISLQEHLNQQLAGMLLGPEDRAA
LHVLIESLNEDGYLEDSLEELAEALLGGEADAETREELLDRLRCALKWLQSLEPAGVGARDLPECLLLQLRAQPRCQAQA
VAILICKQYLELLAKRDAKRLMSLTGADEALLREAQALITALEPKPGRPFSRNQAQAVVPDVIVQKVGREFRVLLNPDVA
PKLRINELYAQALRATRGGVSNTALGAQLQEARWFIKNIQQRFDTILRVSRAIVERQKGFFTHGELAMKPLVLREIADEL
GLHESTISRVTNAKYMATRFGTFELKYFFGSGLSTEAGGNASSTAVRALIKQFVAAEDPAKPLSDSALSNMLEEQGIQVA
RRTVAKYREALKIAPANLRKSL

Nucleotide


Download         Length: 1509 bp        

>NTDB_id=428692 G8A07_RS25870 WP_195794776.1 5618178..5619686(+) (rpoN/rpoN1) [Roseateles sp. DAIF2]
ATGAAGCCCTCCCTGCAGGTCCGCCTCAGCCAGCATCTGGCCCTGACGCCCCAGCTGCAGCAGTCGATCCGGCTGCTGCA
GCTCTCGACCCTGGAGCTGAACCAGGAGGTCGAGCAGATGCTGGCGCAGAACCCGCTGCTGGAGACCGATGAGGAGTTCG
CCGACCAGCCCGCGCCCGAAATGCCCGAGCCGCGCAGCAGCGCCAGCAACGACGAGCCGAGCGGCCAGGCCGCGGATGCC
GAGGCCCCGAGCGAGGAGATGCGCGCCGAGGACTTCGGCACCACCGAGCGCGAGGACTGGGAGAATGGCACCGAGCGCGA
TGACTTCGACGGCATTCGCGAGCTGCCCGGCAGCGGCGCGAGCGGCAATGGCGACGAGGATGGCGAGCGCCCCGAGCAGG
AGGCGCCGTCGATCAGCCTGCAGGAGCATCTGAACCAGCAGCTGGCCGGCATGCTGCTGGGCCCCGAGGACCGCGCCGCG
CTGCATGTGCTGATCGAGTCGCTCAACGAGGACGGCTATCTGGAGGACAGCCTCGAGGAGCTGGCCGAGGCGCTGCTCGG
CGGGGAGGCCGATGCCGAGACCCGCGAGGAACTGCTGGACCGGCTGCGCTGCGCGCTGAAATGGCTGCAGAGCCTGGAGC
CCGCCGGCGTCGGCGCGCGCGACCTGCCCGAATGCCTGCTGCTGCAGCTGCGCGCCCAGCCGCGCTGCCAGGCCCAGGCG
GTGGCGATCCTGATCTGCAAGCAATACCTGGAGCTGCTGGCCAAGCGCGATGCGAAGCGCCTGATGTCGCTGACCGGCGC
CGACGAAGCCCTGCTGCGCGAGGCCCAGGCCCTGATCACCGCGCTGGAGCCCAAGCCCGGCCGGCCCTTCTCGCGCAACC
AGGCGCAGGCCGTCGTGCCCGACGTGATCGTGCAGAAGGTCGGGCGCGAGTTCCGCGTGCTGCTCAATCCCGATGTGGCG
CCCAAGCTGCGCATCAACGAGCTCTATGCCCAAGCCCTGCGCGCCACGCGCGGCGGCGTCAGCAACACCGCGCTGGGCGC
CCAGCTGCAGGAGGCGCGCTGGTTCATCAAGAACATCCAGCAGCGCTTCGACACCATCCTGCGCGTCTCGCGGGCCATCG
TCGAGCGCCAGAAGGGCTTCTTCACCCATGGCGAGCTGGCGATGAAGCCGCTGGTGCTCCGAGAAATAGCCGACGAGCTG
GGCCTGCACGAATCGACCATCTCGCGCGTCACCAACGCCAAGTACATGGCGACCCGCTTCGGCACCTTCGAGCTGAAGTA
CTTCTTCGGCTCCGGCCTCTCGACCGAGGCCGGCGGCAACGCCTCCAGCACCGCGGTACGCGCGCTGATCAAGCAGTTCG
TCGCCGCCGAGGACCCGGCCAAGCCGCTCTCGGACAGCGCGCTGTCGAACATGCTGGAGGAGCAGGGCATCCAGGTCGCG
CGCCGCACCGTCGCCAAGTACCGCGAGGCGCTGAAGATCGCGCCGGCGAACCTGCGCAAATCGCTCTAA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(135-329)

Sigma-54, DNA binding domain (Sigma54_DBD)

(346-500)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7S9CBN1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

54.883

100

0.56