Detailed information    

insolico Bioinformatically predicted

Overview

Name   rapF   Type   Regulator
Locus tag   V753_RS17385 Genome accession   NZ_CP049904
Coordinates   3536223..3537368 (+) Length   381 a.a.
NCBI ID   WP_007407638.1    Uniprot ID   -
Organism   Bacillus velezensis strain GB03     
Function   inhibit the DNA-binding function of ComA (predicted from homology)   
Competence regulation

Genomic Context

Location: 3531223..3542368
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V753_RS17365 (V753_17325) - 3532131..3532559 (-) 429 WP_007614418.1 DUF1934 domain-containing protein -
  V753_RS17370 (V753_17330) - 3532716..3533214 (+) 499 Protein_3370 bacteriocin biosynthesis protein AlbG -
  V753_RS17375 (V753_17335) - 3533221..3534597 (-) 1377 WP_007614432.1 YncE family protein -
  V753_RS17380 (V753_17340) - 3534637..3535992 (-) 1356 WP_007614434.1 YncE family protein -
  V753_RS17385 (V753_17345) rapF 3536223..3537368 (+) 1146 WP_007407638.1 tetratricopeptide repeat protein Regulator
  V753_RS17390 (V753_17350) - 3537352..3537471 (+) 120 WP_021494790.1 PhrC/PhrF family phosphatase-inhibitory pheromone -
  V753_RS17395 (V753_17355) - 3537669..3537872 (+) 204 WP_003151064.1 helix-turn-helix transcriptional regulator -
  V753_RS17400 (V753_17360) - 3537886..3538089 (+) 204 WP_007407641.1 hypothetical protein -
  V753_RS17405 (V753_17365) - 3538124..3538309 (+) 186 WP_007407642.1 DUF4177 domain-containing protein -
  V753_RS17410 (V753_17370) - 3538495..3538776 (+) 282 WP_007407643.1 hypothetical protein -
  V753_RS17415 (V753_17375) - 3538820..3539332 (+) 513 WP_007614439.1 stage II sporulation protein M -
  V753_RS17420 (V753_17385) - 3539923..3540141 (-) 219 WP_003151056.1 helix-turn-helix transcriptional regulator -
  V753_RS17425 (V753_17390) speB 3540308..3541180 (-) 873 WP_007614448.1 agmatinase -
  V753_RS17430 (V753_17395) speE 3541239..3542069 (-) 831 WP_003151052.1 spermidine synthase -

Sequence

Protein


Download         Length: 381 a.a.        Molecular weight: 45543.57 Da        Isoelectric Point: 4.5769

>NTDB_id=428532 V753_RS17385 WP_007407638.1 3536223..3537368(+) (rapF) [Bacillus velezensis strain GB03]
MASVLSSSAVGEKINEWYMYIRRFSIPDAEYLRREIKEELAGLEDDQDLHLYYSLMEFRHNLMLEYLEPLESQRIEEQPR
LSDLLADIDKKQARLTGRLDYYFNFFRGMYELECREYLSAIQFFKKAEYKLDFVKDCIEKAEFYFKMSESYYYMKQTYFS
MDYARQAYKIYHKQEAYNIRVLQCHSLFATNFLDLKQYDEAIQHFKKAYAMAEAEQQPQLMGRTLYNIGLCFNSQENYKP
AIDYIKRAIAVFEDGNIITSLPQAYFLITQIHYKIGNMAIARQYHDKGVSYAEEAEDSLYIAEYEFLESLYVGEPDEEAI
MECFDFLRDKSMYADLEDFALDVAKYYHERENFEKASAYFLKVEETRQQIQGGVKLYEIEV

Nucleotide


Download         Length: 1146 bp        

>NTDB_id=428532 V753_RS17385 WP_007407638.1 3536223..3537368(+) (rapF) [Bacillus velezensis strain GB03]
GTGGCGAGTGTTTTATCATCTTCAGCGGTTGGAGAAAAAATCAACGAATGGTATATGTACATTCGAAGGTTCAGCATCCC
GGACGCCGAGTACTTGCGGAGAGAAATCAAAGAAGAACTTGCAGGTCTGGAAGATGATCAGGATCTTCATCTATACTATT
CTCTCATGGAATTCCGCCACAATCTGATGCTCGAATACCTCGAACCGCTGGAATCGCAGCGGATAGAAGAACAGCCGAGA
CTATCGGATCTGCTCGCGGATATTGATAAAAAGCAGGCCCGGCTGACCGGACGTCTTGATTATTATTTTAATTTTTTCCG
GGGGATGTATGAGCTGGAATGCCGTGAATATCTTTCAGCGATTCAGTTTTTTAAGAAAGCTGAATATAAGCTTGATTTTG
TCAAAGATTGTATCGAGAAAGCTGAATTTTATTTTAAGATGTCAGAGTCCTACTATTATATGAAACAGACTTATTTTTCT
ATGGATTATGCCCGGCAGGCTTATAAAATTTATCATAAGCAGGAGGCATATAATATAAGGGTGCTTCAGTGCCATTCATT
GTTCGCTACCAACTTTCTTGACTTAAAGCAATATGATGAAGCAATTCAGCATTTTAAAAAGGCTTATGCCATGGCAGAGG
CAGAACAGCAGCCACAATTAATGGGCCGGACTCTATATAATATCGGACTTTGTTTTAACAGCCAAGAAAATTACAAACCA
GCCATTGATTACATAAAACGGGCAATAGCTGTTTTTGAAGATGGGAACATCATCACTTCTCTCCCGCAAGCCTATTTCTT
AATCACACAAATACATTATAAAATCGGAAATATGGCCATAGCCAGACAATATCATGACAAAGGGGTATCTTATGCAGAGG
AGGCAGAAGATTCCCTATATATCGCCGAGTATGAATTTTTAGAATCTTTATATGTCGGTGAGCCTGATGAAGAAGCAATC
ATGGAATGTTTTGACTTTCTCAGAGATAAATCGATGTATGCTGATCTTGAAGATTTTGCCTTAGATGTGGCAAAATATTA
TCATGAAAGAGAAAATTTCGAGAAGGCTTCCGCATATTTTTTAAAGGTGGAAGAAACAAGACAGCAGATTCAAGGAGGTG
TGAAGTTGTATGAAATTGAAGTATAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_12)

(189-253)

response regulator aspartate phosphatase H, N terminal (RapH_N)

(5-65)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rapF Bacillus subtilis subsp. subtilis str. 168

78.478

100

0.785
  rapC Bacillus subtilis subsp. subtilis str. 168

56.579

99.738

0.564