Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   V753_RS12085 Genome accession   NZ_CP049904
Coordinates   2508528..2509142 (-) Length   204 a.a.
NCBI ID   WP_007612692.1    Uniprot ID   -
Organism   Bacillus velezensis strain GB03     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2503528..2514142
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V753_RS12055 (V753_12030) rpsT 2503831..2504097 (+) 267 WP_003152876.1 30S ribosomal protein S20 -
  V753_RS12060 (V753_12035) holA 2504114..2505155 (-) 1042 Protein_2332 DNA polymerase III subunit delta -
  V753_RS12065 (V753_12040) - 2505195..2505346 (-) 152 Protein_2333 hypothetical protein -
  V753_RS12070 (V753_12045) - 2505387..2505521 (+) 135 WP_029326064.1 YqzM family protein -
  V753_RS12075 (V753_12050) comEC 2505540..2507891 (-) 2352 WP_007612689.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  V753_RS12080 (V753_12055) - 2507892..2508461 (-) 570 WP_003152868.1 ComE operon protein 2 -
  V753_RS12085 (V753_12060) comEA 2508528..2509142 (-) 615 WP_007612692.1 helix-hairpin-helix domain-containing protein Machinery gene
  V753_RS12090 (V753_12065) comER 2509201..2510022 (+) 822 WP_007612693.1 late competence protein ComER -
  V753_RS12095 (V753_12070) - 2510091..2510828 (-) 738 WP_007612694.1 class I SAM-dependent DNA methyltransferase -
  V753_RS12100 (V753_12075) rsfS 2510825..2511181 (-) 357 WP_007408260.1 ribosome silencing factor -
  V753_RS12105 (V753_12080) yqeK 2511199..2511759 (-) 561 WP_014418422.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  V753_RS12110 (V753_12085) - 2511749..2512318 (-) 570 WP_007408258.1 nicotinate-nucleotide adenylyltransferase -
  V753_RS12115 (V753_12090) yhbY 2512329..2512619 (-) 291 WP_007408257.1 ribosome assembly RNA-binding protein YhbY -
  V753_RS12120 (V753_12095) aroE 2512613..2513455 (-) 843 WP_003152856.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 204 a.a.        Molecular weight: 22265.52 Da        Isoelectric Point: 7.7754

>NTDB_id=428511 V753_RS12085 WP_007612692.1 2508528..2509142(-) (comEA) [Bacillus velezensis strain GB03]
MTKAMMTERLRRNLKILGACLVAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELKVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREEKGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide


Download         Length: 615 bp        

>NTDB_id=428511 V753_RS12085 WP_007612692.1 2508528..2509142(-) (comEA) [Bacillus velezensis strain GB03]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGTAGCAGGGGCGGTTTT
TATCTGCATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGACAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAAAGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAAAGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

SLBB domain (SLBB)

(68-122)

Helix-hairpin-helix motif (HHH_3)

(141-202)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

58.115

93.627

0.544
  comEA Latilactobacillus sakei subsp. sakei 23K

31.897

100

0.363
  comEA/celA/cilE Streptococcus pneumoniae TIGR4

35.071

100

0.363