Detailed information NTG0042622

Bioinformatically predicted

Overview

• Name: rapF
• Type: Regulator
• Locus tag: GYO_RS41215
• Genome accession: NC_016047
• Coordinates: 3832032..3833177 (+)
• Length: 381 a.a.
• NCBI ID: WP_014115555.1
• Uniprot ID: G4NXJ1
• Organism: Bacillus spizizenii TU-B-10
• Function: inhibit the DNA-binding function of ComA (predicted from homology) Competence regulation

Genomic Context

Location: 3827032..3838177

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GYO_RS41190 (GYO_4121)	-	3827166..3828326 (+)	1161	WP_079287694.1	insulinase family protein	-
GYO_RS41195 (GYO_4122)	-	3828331..3829614 (+)	1284	WP_014115551.1	pitrilysin family protein	-
GYO_RS41200 (GYO_4123)	-	3829611..3830312 (+)	702	WP_014115552.1	bacteriocin biosynthesis protein AlbG	-
GYO_RS41205 (GYO_4124)	-	3830450..3831805 (-)	1356	WP_014115553.1	YncE family protein	-
GYO_RS41215 (GYO_4126)	rapF	3832032..3833177 (+)	1146	WP_014115555.1	response regulator aspartate phosphatase RapF	Regulator
GYO_RS41220	phrF	3833161..3833280 (+)	120	WP_009968329.1	phosphatase RapF inhibitor PhrF	Regulator
GYO_RS41225	-	3833521..3834000 (+)	480	WP_041521094.1	hypothetical protein	-
GYO_RS41230 (GYO_4128)	-	3834150..3834938 (+)	789	WP_014115557.1	membrane protein	-
GYO_RS41235 (GYO_4129)	-	3834925..3835599 (+)	675	WP_014115558.1	hypothetical protein	-
GYO_RS41240 (GYO_4130)	-	3835609..3836406 (+)	798	WP_238528886.1	hypothetical protein	-
GYO_RS41245 (GYO_4131)	-	3836409..3837056 (+)	648	WP_014115560.1	ABC transporter ATP-binding protein	-
GYO_RS41250 (GYO_4132)	-	3837049..3837609 (+)	561	WP_014115561.1	hypothetical protein	-

Sequence

Protein

• Length: 381 a.a.
• Molecular weight: 45663.12 Da
• Isoelectric Point: 5.1517

>NTDB_id=42622 GYO_RS41215 WP_014115555.1 3832032..3833177(+) (rapF) [Bacillus spizizenii TU-B-10]
MTGVISSSSVGEKINEWYMYIRRFSIPDAEYLRREIKQELDQMEEDQDLHLYYSLMEFRHNLMLEYLEPLEKMRIEEQPR
LSDLLIEIDKKQARLTGVLDYYFNFFRGMYELDQREYLSAIKFFKKAESKLVFVKDRIEKAEFFFKMSESYYYMKQTYFS
MDYARQAYEIYKEHEAYNIRLLQCHSLFATNFLDLKQYEDAISHFQKAYSMAEAENQPQLMGRTLYNIGLCKNSQSQYED
AITYFKRAIAVFEESNILPSLPQAYFLITQIHYKLGKMDKAREYHSKGMAYSQKAGDLIYLSEFEFLKSLYLSGPDEEAI
QGFFDFLESKMLYADLEDFALDVAKYYHERKNFQKASAYFLKVEQVRQLIQGGVRLYEIEV

Nucleotide

• Length: 1146 bp

>NTDB_id=42622 GYO_RS41215 WP_014115555.1 3832032..3833177(+) (rapF) [Bacillus spizizenii TU-B-10]
GTGACAGGTGTCATATCTTCTTCTTCCGTCGGAGAAAAGATTAACGAATGGTATATGTACATACGCCGATTCAGCATACC
CGATGCAGAGTATTTGCGAAGAGAAATCAAACAAGAGCTGGATCAAATGGAAGAAGATCAGGATCTTCATTTGTACTATT
CACTGATGGAATTCCGGCACAACCTGATGCTTGAGTACCTTGAACCACTAGAGAAAATGCGGATCGAGGAACAGCCGAGA
TTGTCAGATTTGCTGATTGAGATTGATAAAAAACAGGCGCGTTTAACTGGAGTCCTTGATTATTACTTTAATTTCTTCAG
GGGCATGTATGAGCTAGACCAGCGGGAATATCTGTCGGCTATTAAATTTTTTAAAAAGGCCGAAAGCAAGTTGGTATTTG
TTAAGGATCGGATAGAGAAGGCTGAGTTTTTCTTTAAGATGTCTGAATCTTATTACTATATGAAACAAACGTATTTTTCA
ATGGACTATGCACGGCAGGCATATGAAATATACAAAGAACATGAAGCTTATAATATAAGATTGCTGCAGTGTCATTCTTT
ATTTGCCACCAATTTTTTAGATTTAAAACAGTATGAGGATGCCATCTCACATTTTCAAAAAGCTTACTCTATGGCAGAAG
CTGAAAATCAGCCCCAATTAATGGGAAGAACATTGTACAATATCGGGCTTTGTAAAAACAGCCAAAGCCAATATGAGGAT
GCCATAACTTATTTCAAAAGAGCAATAGCTGTTTTTGAAGAATCAAATATTCTTCCTTCCTTACCTCAAGCGTATTTTTT
AATTACACAGATCCATTATAAATTAGGAAAAATGGATAAAGCCCGTGAATATCATAGTAAGGGAATGGCTTATTCTCAAA
AGGCCGGAGATTTAATATATTTATCGGAGTTTGAATTTTTGAAATCTTTATACTTATCAGGCCCTGATGAAGAAGCAATT
CAAGGATTTTTTGATTTTCTCGAAAGTAAAATGTTGTATGCTGATCTTGAAGATTTCGCTCTTGATGTGGCAAAATATTA
TCATGAACGTAAAAATTTTCAAAAAGCTTCTGCTTATTTTCTGAAGGTGGAACAAGTGAGGCAACTTATTCAAGGAGGAG
TGAGATTGTATGAAATTGAAGTCTAA

Domains

Predicted by InterproScan.

• Anaphase-promoting complex, cyclosome, subunit 3 (ANAPC3) (133-208)

• response regulator aspartate phosphatase H, N terminal (RapH_N) (4-65)

• Tetratricopeptide repeat (TPR_12) (223-290)

3D structure

• AlphaFold DB: G4NXJ1

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
rapF	Bacillus subtilis subsp. subtilis str. 168	96.85	100	0.969
rapC	Bacillus subtilis subsp. subtilis str. 168	57.895	99.738	0.577