Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   G5S32_RS01185 Genome accession   NZ_CP049331
Coordinates   269706..270305 (+) Length   199 a.a.
NCBI ID   WP_165310110.1    Uniprot ID   A0A6G7CF05
Organism   Vibrio ziniensis strain ZWAL4003     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 264706..275305
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G5S32_RS01170 (G5S32_01170) - 265509..267983 (-) 2475 WP_165310107.1 PBP1A family penicillin-binding protein -
  G5S32_RS01175 (G5S32_01175) pilM 268126..269136 (+) 1011 WP_165310108.1 type IV pilus assembly protein PilM Machinery gene
  G5S32_RS01180 (G5S32_01180) - 269126..269713 (+) 588 WP_165310109.1 PilN domain-containing protein -
  G5S32_RS01185 (G5S32_01185) pilO 269706..270305 (+) 600 WP_165310110.1 type 4a pilus biogenesis protein PilO Machinery gene
  G5S32_RS01190 (G5S32_01190) - 270302..270838 (+) 537 WP_165310111.1 pilus assembly protein PilP -
  G5S32_RS01195 (G5S32_01195) pilQ 270966..272243 (+) 1278 Protein_217 type IV pilus secretin PilQ -
  G5S32_RS01200 (G5S32_01200) aroK 272430..272951 (+) 522 WP_165310113.1 shikimate kinase AroK -
  G5S32_RS01205 (G5S32_01205) aroB 272979..274064 (+) 1086 WP_165310114.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 199 a.a.        Molecular weight: 23066.49 Da        Isoelectric Point: 5.0805

>NTDB_id=426143 G5S32_RS01185 WP_165310110.1 269706..270305(+) (pilO) [Vibrio ziniensis strain ZWAL4003]
MGDFNNLEIDDIAEWPLLPQLVMLLIVALLIQGVGYWFYLTPKQNTLHLLIEEEQTLKSAIRTKAHKVANLPQVKAQLDD
LSERYDDLLQQLPEQKELATLLAAVNELGNKHQLKIIRIEWGEKEQQAFLSRMPLNLELTGEYHNIGLFSQAIAQLPRII
HLEDVNWQRIRHDSENLSVQVRAFTYQYHPDVVNKGELQ

Nucleotide


Download         Length: 600 bp        

>NTDB_id=426143 G5S32_RS01185 WP_165310110.1 269706..270305(+) (pilO) [Vibrio ziniensis strain ZWAL4003]
ATGGGTGATTTTAATAACCTAGAGATCGACGATATTGCAGAGTGGCCACTGCTACCTCAGCTTGTCATGTTATTGATTGT
TGCTTTGTTGATTCAAGGTGTGGGGTATTGGTTCTATCTCACGCCTAAGCAAAACACTTTGCATCTTCTTATTGAAGAGG
AGCAAACACTCAAATCAGCGATCCGCACTAAAGCCCATAAGGTCGCTAATCTGCCGCAAGTCAAAGCTCAACTTGATGAT
TTGTCAGAGCGTTATGATGACCTTTTACAGCAACTGCCTGAACAGAAGGAACTCGCTACTCTGCTAGCGGCTGTGAATGA
ACTCGGAAATAAACATCAATTAAAGATCATTCGTATTGAATGGGGAGAAAAAGAGCAGCAAGCGTTCTTGTCTCGCATGC
CATTAAACTTAGAGCTAACAGGTGAATACCACAATATTGGCTTATTTTCACAAGCTATCGCTCAGTTGCCTCGGATAATC
CATTTAGAAGACGTTAACTGGCAAAGAATCCGTCATGACAGCGAAAACTTAAGCGTTCAGGTACGAGCGTTTACCTATCA
ATATCATCCTGATGTCGTTAACAAAGGTGAGCTGCAATGA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(52-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6G7CF05

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio campbellii strain DS40M4

61.053

95.477

0.583
  pilO Vibrio cholerae strain A1552

60.417

96.482

0.583