Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   GYO_RS22145 Genome accession   NC_016047
Coordinates   106175..107551 (+) Length   458 a.a.
NCBI ID   WP_014112330.1    Uniprot ID   G4NQC1
Organism   Bacillus spizizenii TU-B-10     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 101175..112551
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GYO_RS22125 (GYO_0109) ctsR 101528..101992 (+) 465 WP_014112327.1 transcriptional regulator CtsR -
  GYO_RS22130 (GYO_0110) mcsA 102006..102563 (+) 558 WP_014112328.1 protein-arginine kinase activator protein McsA -
  GYO_RS22135 (GYO_0111) - 102563..103654 (+) 1092 WP_014112329.1 protein arginine kinase -
  GYO_RS22140 (GYO_0112) clpC 103651..106083 (+) 2433 WP_003225731.1 ATP-dependent protease ATP-binding subunit ClpC Regulator
  GYO_RS22145 (GYO_0113) radA 106175..107551 (+) 1377 WP_014112330.1 DNA repair protein RadA Machinery gene
  GYO_RS22150 (GYO_0114) disA 107555..108637 (+) 1083 WP_014112331.1 DNA integrity scanning diadenylate cyclase DisA -
  GYO_RS22155 (GYO_0115) - 108752..109852 (+) 1101 WP_014112332.1 PIN/TRAM domain-containing protein -
  GYO_RS22160 (GYO_0116) ispD 109866..110564 (+) 699 WP_014112333.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  GYO_RS22165 (GYO_0117) ispF 110557..111033 (+) 477 WP_014112334.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 49441.94 Da        Isoelectric Point: 7.9801

>NTDB_id=42553 GYO_RS22145 WP_014112330.1 106175..107551(+) (radA) [Bacillus spizizenii TU-B-10]
MAKTKSKFICQSCGYESPKWMGKCPGCGAWNTMVEEMIKKAPANRRAAFSHSVQTVQKPSPITSIETSEEPRVKTQLGEF
NRVLGGGVVKGSLVLIGGDPGIGKSTLLLQVSAQLSDSSNSVLYISGEESVKQTKLRADRLGINSPSLHVLSETDMEYIS
SAIQEMNPAFVVVDSIQTVYQSDITSAPGSVSQVRECTAELMKIAKTKGIPIFIVGHVTKEGSIAGPRLLEHMVDTVLYF
EGERHHTFRILRAVKNRFGSTNEMGIFEMREEGLTEVLNPSEIFLEERSAGSAGSSITASMEGTRPILVEIQALISPTSF
GNPRRMATGIDHNRVSLLMAVLEKRVGLLLQNQDAYLKVAGGVKLDEPAIDLAIAISIASSFKDTPPNPADCFIGEVGLT
GEVRRVSRIEQRVKEAAKLGFKRMIIPAANLDGWTKPKGIEVIGVANVAEALRTSLGG

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=42553 GYO_RS22145 WP_014112330.1 106175..107551(+) (radA) [Bacillus spizizenii TU-B-10]
ATGGCTAAAACAAAATCGAAATTCATCTGCCAATCCTGCGGTTACGAGTCTCCAAAATGGATGGGGAAATGTCCGGGCTG
CGGTGCTTGGAATACTATGGTGGAAGAAATGATAAAAAAAGCGCCGGCCAATCGGAGAGCGGCTTTTTCTCATTCAGTTC
AAACTGTACAGAAACCTTCACCTATTACATCAATCGAAACATCAGAAGAACCCCGGGTTAAAACTCAGCTTGGCGAGTTT
AACAGGGTGCTCGGCGGGGGCGTCGTCAAAGGCTCACTCGTTTTAATCGGCGGCGATCCTGGTATCGGAAAGTCAACGTT
ATTACTGCAGGTTTCCGCTCAATTATCAGATTCATCGAACAGTGTGCTGTACATTTCGGGAGAAGAATCGGTAAAGCAAA
CGAAACTGAGAGCAGATCGTCTTGGCATTAATAGTCCGTCACTGCATGTTTTATCTGAAACCGATATGGAGTATATTTCG
TCTGCTATACAAGAGATGAATCCAGCGTTTGTCGTTGTAGACTCTATTCAAACGGTTTACCAAAGTGACATTACATCGGC
TCCGGGCAGTGTGTCACAGGTCAGAGAATGTACCGCTGAGCTGATGAAAATTGCAAAAACAAAAGGCATTCCGATTTTTA
TCGTAGGGCACGTGACGAAAGAAGGGTCTATTGCAGGGCCGAGACTGTTGGAGCATATGGTTGACACTGTTTTATATTTT
GAAGGGGAACGTCACCATACTTTCCGTATTTTGCGGGCTGTAAAAAACCGTTTTGGTTCTACAAATGAAATGGGCATTTT
CGAAATGCGCGAAGAGGGGCTCACTGAAGTTTTGAACCCTTCAGAAATTTTCTTGGAAGAACGCTCAGCGGGATCCGCAG
GCTCAAGCATAACTGCTTCTATGGAGGGCACAAGACCGATTCTCGTTGAAATCCAGGCGCTCATCTCGCCAACAAGCTTT
GGCAATCCGCGGCGTATGGCAACAGGAATAGACCATAACAGGGTTTCTCTGTTAATGGCCGTGTTAGAAAAAAGAGTTGG
GCTGCTGCTGCAAAATCAGGATGCTTATTTGAAAGTGGCCGGCGGCGTAAAGCTTGATGAACCTGCAATTGATCTTGCCA
TTGCGATCAGCATCGCGTCCAGCTTTAAAGATACACCTCCAAATCCTGCAGATTGTTTTATAGGAGAAGTGGGATTAACC
GGAGAAGTTCGGCGGGTTTCAAGAATTGAACAGCGTGTGAAAGAAGCGGCTAAGCTCGGTTTTAAACGAATGATCATACC
CGCGGCAAATCTGGATGGATGGACAAAACCAAAAGGGATTGAGGTTATCGGAGTAGCAAATGTTGCAGAGGCGCTTCGTA
CTTCATTAGGAGGATAA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(365-430)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(72-222)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G4NQC1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

98.908

100

0.989
  radA Streptococcus pneumoniae Rx1

63.576

98.908

0.629
  radA Streptococcus pneumoniae D39

63.576

98.908

0.629
  radA Streptococcus pneumoniae R6

63.576

98.908

0.629
  radA Streptococcus pneumoniae TIGR4

63.576

98.908

0.629
  radA Streptococcus mitis NCTC 12261

63.576

98.908

0.629
  radA Streptococcus mitis SK321

63.355

98.908

0.627

Multiple sequence alignment