Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   G5S31_RS02360 Genome accession   NZ_CP049090
Coordinates   452316..452897 (-) Length   193 a.a.
NCBI ID   WP_021110392.1    Uniprot ID   A0A084ELH5
Organism   Glaesserella parasuis strain aHPS7     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 447316..457897
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G5S31_RS02320 (G5S31_02315) - 447661..448299 (+) 639 WP_160405279.1 nitroreductase family protein -
  G5S31_RS02325 (G5S31_02320) - 448388..448921 (+) 534 WP_010786060.1 TIGR00645 family protein -
  G5S31_RS02330 (G5S31_02325) - 449006..450304 (-) 1299 WP_021118667.1 NAD(P)/FAD-dependent oxidoreductase -
  G5S31_RS02350 (G5S31_02345) greB 450832..451320 (-) 489 WP_035496521.1 transcription elongation factor GreB -
  G5S31_RS02355 (G5S31_02350) - 451320..452255 (-) 936 WP_005710401.1 KpsF/GutQ family sugar-phosphate isomerase -
  G5S31_RS02360 (G5S31_02355) pilF 452316..452897 (-) 582 WP_021110392.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  G5S31_RS02365 (G5S31_02360) - 452997..454157 (-) 1161 WP_010786053.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  G5S31_RS02370 (G5S31_02365) - 454358..454666 (-) 309 WP_005710405.1 helix-turn-helix domain-containing protein -
  G5S31_RS11595 - 454807..455007 (-) 201 WP_021119412.1 hypothetical protein -
  G5S31_RS02400 (G5S31_02395) gltX 455846..457285 (+) 1440 WP_160421844.1 glutamate--tRNA ligase -

Sequence

Protein


Download         Length: 193 a.a.        Molecular weight: 22339.24 Da        Isoelectric Point: 8.0872

>NTDB_id=424666 G5S31_RS02360 WP_021110392.1 452316..452897(-) (pilF) [Glaesserella parasuis strain aHPS7]
MTFAKFFRNLTACSVALWLVACANQTTQVDFNRSEAVKARINLALAYLEQSDFPKAKENIDKALEHDVKDYLPHSVLAYY
YQQTGDNQKAEEAYQQALKLSKTQSKNNQVRPDVLNNYGTFLCKQKQFDKAYQQFETALTSQEAYYNQADTLENIALCAN
MKKDIEKQKTALSQLEKIDKSRAERLYNFMEIR

Nucleotide


Download         Length: 582 bp        

>NTDB_id=424666 G5S31_RS02360 WP_021110392.1 452316..452897(-) (pilF) [Glaesserella parasuis strain aHPS7]
ATGACCTTTGCAAAATTTTTCCGTAATTTGACCGCTTGTAGCGTGGCATTATGGCTTGTTGCTTGTGCGAACCAAACTAC
TCAGGTGGATTTTAACCGCTCGGAAGCCGTCAAAGCTCGCATTAATTTGGCCCTTGCTTACCTTGAACAATCTGATTTTC
CAAAAGCGAAAGAGAACATTGACAAAGCGCTTGAACACGATGTAAAAGACTACCTACCGCACTCTGTGCTTGCCTATTAT
TACCAACAAACTGGCGATAACCAAAAAGCAGAAGAAGCCTACCAGCAAGCCTTGAAGTTAAGTAAAACACAGAGCAAAAA
CAATCAAGTTCGCCCTGATGTATTGAATAACTACGGTACCTTTCTGTGCAAACAAAAACAGTTCGACAAAGCCTATCAGC
AATTTGAAACGGCATTAACCAGTCAAGAAGCCTATTACAATCAAGCAGATACACTAGAAAATATCGCCTTATGTGCCAAT
ATGAAGAAGGATATTGAGAAGCAAAAAACAGCACTATCTCAACTTGAGAAAATAGATAAATCAAGAGCAGAACGACTATA
TAATTTCATGGAGATTAGATAG

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_2)

(74-102)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A084ELH5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Glaesserella parasuis strain SC1401

99.482

100

0.995
  pilF/pilF2 Haemophilus influenzae Rd KW20

44.693

92.746

0.415