Detailed information NTG0424270

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: G5B45_RS23275
• Genome accession: NZ_CP048933
• Coordinates: 4557459..4558259 (+)
• Length: 266 a.a.
• NCBI ID: WP_002888947.1
• Uniprot ID: -
• Organism: Klebsiella pneumoniae strain KP18-2079
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4552459..4563259

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
G5B45_RS23240 (G5B45_23210)	gspH	4552615..4553130 (+)	516	WP_004151939.1	type II secretion system minor pseudopilin GspH	-
G5B45_RS23245 (G5B45_23215)	gspI	4553127..4553492 (+)	366	WP_002889025.1	type II secretion system minor pseudopilin GspI	-
G5B45_RS23250 (G5B45_23220)	gspJ	4553489..4554085 (+)	597	WP_004151940.1	type II secretion system minor pseudopilin GspJ	-
G5B45_RS23255 (G5B45_23225)	gspK	4554082..4555068 (+)	987	WP_004151941.1	type II secretion system minor pseudopilin GspK	-
G5B45_RS23260 (G5B45_23230)	gspL	4555055..4556248 (+)	1194	WP_004151942.1	type II secretion system protein GspL	-
G5B45_RS23265 (G5B45_23235)	-	4556241..4556726 (+)	486	WP_004151943.1	type II secretion system protein M	-
G5B45_RS23270 (G5B45_23240)	-	4556716..4557462 (+)	747	WP_004145897.1	type II secretion system protein N	-
G5B45_RS23275 (G5B45_23245)	pilD	4557459..4558259 (+)	801	WP_002888947.1	prepilin peptidase	Machinery gene
G5B45_RS23280 (G5B45_23250)	hrpB	4558307..4560736 (-)	2430	WP_004151944.1	ATP-dependent helicase HrpB	-
G5B45_RS23285 (G5B45_23255)	thpR	4560809..4561345 (+)	537	WP_004145901.1	RNA 2',3'-cyclic phosphodiesterase	-
G5B45_RS23290 (G5B45_23260)	sfsA	4561345..4562061 (+)	717	WP_002888848.1	DNA/RNA nuclease SfsA	-
G5B45_RS23295 (G5B45_23265)	dksA	4562224..4562679 (+)	456	WP_002888845.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29419.04 Da
• Isoelectric Point: 8.8894

>NTDB_id=424270 G5B45_RS23275 WP_002888947.1 4557459..4558259(+) (pilD) [Klebsiella pneumoniae strain KP18-2079]
MTTLAALSLHFPFVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLVLLSFLLILARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASASGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGSIFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=424270 G5B45_RS23275 WP_002888947.1 4557459..4558259(+) (pilD) [Klebsiella pneumoniae strain KP18-2079]
ATGACGACACTAGCCGCGTTATCGCTGCACTTTCCCTTTGTCTGGTATGGCTTTCTACTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAATGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGCTCTTCCTGCCCGCAGTGCCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGAGCTTTGCTAGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGGTGCTGCTCTCGTTCCTGCTGATACTGGCGA
GGATTGACGCCCGGACCCAGCTGCTGCCCGATCGCCTGACTCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGATGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCTTCGGCCAGCGGGCTGGTGTGGACCCTGCTGCAACGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGCGGGAGTATTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	46.182	100	0.477
pilD	Acinetobacter nosocomialis M2	46.008	98.872	0.455
pilD	Acinetobacter baumannii D1279779	45.247	98.872	0.447
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425