Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   IFO96_RS03630 Genome accession   NZ_CP062211
Coordinates   749636..750076 (-) Length   146 a.a.
NCBI ID   WP_000360895.1    Uniprot ID   B7LFX5
Organism   Escherichia coli strain L3Cip3     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 744636..755076
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IFO96_RS03605 (IFO96_03605) coaE 745126..745746 (-) 621 WP_001269520.1 dephospho-CoA kinase -
  IFO96_RS03610 (IFO96_03610) - 745771..745815 (+) 45 WP_120795372.1 protein YacM -
  IFO96_RS03615 (IFO96_03615) guaC 745971..747014 (+) 1044 WP_001217338.1 GMP reductase -
  IFO96_RS03620 (IFO96_03620) hofC 747049..748251 (-) 1203 WP_000157236.1 protein transport protein HofC -
  IFO96_RS03625 (IFO96_03625) pilB 748241..749626 (-) 1386 WP_001025174.1 type II secretion system protein GspE Machinery gene
  IFO96_RS03630 (IFO96_03630) pilA 749636..750076 (-) 441 WP_000360895.1 prepilin peptidase-dependent pilin Machinery gene
  IFO96_RS03635 (IFO96_03635) nadC 750279..751172 (-) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  IFO96_RS03640 (IFO96_03640) ampD 751260..751811 (+) 552 WP_100004867.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  IFO96_RS03645 (IFO96_03645) ampE 751808..752662 (+) 855 WP_000172000.1 beta-lactamase regulator AmpE -
  IFO96_RS03650 (IFO96_03650) aroP 752705..754078 (-) 1374 WP_072133621.1 aromatic amino acid transporter AroP -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15602.71 Da        Isoelectric Point: 4.3927

>NTDB_id=424078 IFO96_RS03630 WP_000360895.1 749636..750076(-) (pilA) [Escherichia coli strain L3Cip3]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTH
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=424078 IFO96_RS03630 WP_000360895.1 749636..750076(-) (pilA) [Escherichia coli strain L3Cip3]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCAC
TATGTTTCAGCCATGAGTGTGGCAAAAGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGACTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7LFX5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363