Detailed information    

insolico Bioinformatically predicted

Overview

Name   comL   Type   Machinery gene
Locus tag   IEM32_RS22225 Genome accession   NZ_CP062160
Coordinates   4506174..4506911 (+) Length   245 a.a.
NCBI ID   WP_000197686.1    Uniprot ID   P0AC03
Organism   Escherichia coli strain 20R2R     
Function   DNA binding (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4501174..4511911
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IEM32_RS22210 (IEM32_22210) clpC 4501628..4504201 (-) 2574 WP_001235102.1 ATP-dependent chaperone ClpB Regulator
  IEM32_RS22215 (IEM32_22215) yfiH 4504331..4505062 (-) 732 WP_000040152.1 purine nucleoside phosphorylase YfiH -
  IEM32_RS22220 (IEM32_22220) rluD 4505059..4506039 (-) 981 WP_000079092.1 23S rRNA pseudouridine(1911/1915/1917) synthase RluD -
  IEM32_RS22225 (IEM32_22225) comL 4506174..4506911 (+) 738 WP_000197686.1 outer membrane protein assembly factor BamD Machinery gene
  IEM32_RS22230 (IEM32_22230) raiA 4507182..4507523 (+) 342 WP_000178456.1 ribosome-associated translation inhibitor RaiA -
  IEM32_RS22235 (IEM32_22235) pheL 4507627..4507674 (+) 48 WP_001386991.1 pheA operon leader peptide PheL -
  IEM32_RS22240 (IEM32_22240) pheA 4507773..4508933 (+) 1161 WP_000200120.1 bifunctional chorismate mutase/prephenate dehydratase -
  IEM32_RS22245 (IEM32_22245) tyrA 4508976..4510097 (-) 1122 WP_000225204.1 bifunctional chorismate mutase/prephenate dehydrogenase -
  IEM32_RS22250 (IEM32_22250) aroF 4510108..4511178 (-) 1071 WP_001168032.1 3-deoxy-7-phosphoheptulonate synthase AroF -
  IEM32_RS22255 (IEM32_22255) yfiL 4511388..4511753 (+) 366 WP_001301878.1 DUF2799 domain-containing protein -

Sequence

Protein


Download         Length: 245 a.a.        Molecular weight: 27829.40 Da        Isoelectric Point: 6.4874

>NTDB_id=423885 IEM32_RS22225 WP_000197686.1 4506174..4506911(+) (comL) [Escherichia coli strain 20R2R]
MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQDGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYY
KNADLPLAQAAIDRFIRLNPTHPNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLMENAYRQMQMNAQAEKVAKIIAA
NSSNT

Nucleotide


Download         Length: 738 bp        

>NTDB_id=423885 IEM32_RS22225 WP_000197686.1 4506174..4506911(+) (comL) [Escherichia coli strain 20R2R]
ATGACGCGCATGAAATATCTGGTGGCAGCCGCCACACTAAGCCTGTTTTTGGCGGGTTGCTCGGGGTCAAAGGAAGAAGT
ACCTGATAATCCGCCAAATGAAATTTACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGGCAATAACGC
AACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCGCAGCAGGTGCAGCTGGATCTCATCTACGCCTACTAT
AAAAACGCCGATTTGCCGTTAGCGCAGGCTGCCATCGATCGTTTTATTCGCCTTAACCCGACCCATCCGAATATCGATTA
TGTCATGTACATGCGTGGCCTGACCAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGCGTTGACCGTAGCG
ATCGCGATCCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCAAACAGTCAGTACACC
ACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACTCCGTGGCCGAGTACTATACAGA
ACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATG
CGCTGCCGCTGATGGAAAATGCATACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCA
AACAGCAGCAATACATAA

Domains

Predicted by InterProScan.

Outer membrane lipoprotein (YfiO)

(28-236)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P0AC03

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comL Neisseria meningitidis MC58

38.525

99.592

0.384
  comL Neisseria gonorrhoeae MS11

37.705

99.592

0.376