Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   NH8B_RS16165 Genome accession   NC_016002
Coordinates   3504977..3506380 (+) Length   467 a.a.
NCBI ID   WP_014088221.1    Uniprot ID   G2IYG9
Organism   Pseudogulbenkiania sp. NH8B     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3499977..3511380
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NH8B_RS16140 (NH8B_3324) - 3501662..3502642 (+) 981 WP_008954917.1 KpsF/GutQ family sugar-phosphate isomerase -
  NH8B_RS16145 (NH8B_3325) kdsC 3502651..3503175 (+) 525 WP_014088220.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  NH8B_RS16150 (NH8B_3326) lptC 3503172..3503735 (+) 564 WP_008954915.1 LPS export ABC transporter periplasmic protein LptC -
  NH8B_RS16155 (NH8B_3327) lptA 3503725..3504246 (+) 522 WP_008954914.1 lipopolysaccharide transport periplasmic protein LptA -
  NH8B_RS16160 (NH8B_3328) lptB 3504243..3504971 (+) 729 WP_008954913.1 LPS export ABC transporter ATP-binding protein -
  NH8B_RS16165 (NH8B_3329) rpoN/rpoN1 3504977..3506380 (+) 1404 WP_014088221.1 RNA polymerase factor sigma-54 Machinery gene
  NH8B_RS16170 (NH8B_3330) hpf 3506448..3506777 (+) 330 WP_008954911.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  NH8B_RS16175 (NH8B_3331) ptsN 3506895..3507356 (+) 462 WP_008954910.1 PTS IIA-like nitrogen regulatory protein PtsN -
  NH8B_RS16180 (NH8B_3332) hprK 3507349..3508305 (+) 957 WP_008954909.1 HPr(Ser) kinase/phosphatase -
  NH8B_RS16185 (NH8B_3333) rapZ 3508286..3509128 (+) 843 WP_014088222.1 RNase adapter RapZ -
  NH8B_RS16190 (NH8B_3334) - 3509125..3509493 (+) 369 WP_014088223.1 NusG domain II-containing protein -
  NH8B_RS16195 (NH8B_3335) - 3509474..3510028 (+) 555 WP_014088224.1 Gx transporter family protein -
  NH8B_RS16200 (NH8B_3336) - 3509995..3510630 (+) 636 WP_014088225.1 flavin prenyltransferase UbiX -

Sequence

Protein


Download         Length: 467 a.a.        Molecular weight: 52197.17 Da        Isoelectric Point: 4.6991

>NTDB_id=42372 NH8B_RS16165 WP_014088221.1 3504977..3506380(+) (rpoN/rpoN1) [Pseudogulbenkiania sp. NH8B]
MKQSLQLKVSQQLTLTPQLQQSIRLLQLSTVDLQTEVERFLQDNPLLERGDEPVADSAPAETTGSEAEPERQEPEREDNS
SGELTDWGSGSRRGEDGDDYDPMANVPRPVTLREHLLAQLGEIALPERDCAIVQLLIEELDEDGYLTIDLQELAASLPLE
LGIEEDELAFGLKLLQQFEPQGVGARSLSESLALQLAQLPDSTPGKALAHAIVREHLTLLGNRDYTRLKRLLDVSDAELK
QAQSLIARLNPRPASGFGVDDTRYIIPDIAVRRIKGRWRAELNNAAVPRLRVNQMYAQMLSENRGSGGEMSSRLQEAKWL
VKNIQQRFDTILKVAESIVERQQAFFEHGEVAMRPLILRDIADELELHESTVSRVTTQKYLLCPRGLFELKYFFGSALET
DSGGECSATAIKAHIRQMIDAENRAKPLSDSALADELAKQGIQVARRTVAKYREAMQIPPVNLRKVL

Nucleotide


Download         Length: 1404 bp        

>NTDB_id=42372 NH8B_RS16165 WP_014088221.1 3504977..3506380(+) (rpoN/rpoN1) [Pseudogulbenkiania sp. NH8B]
ATGAAGCAGTCGCTACAGCTCAAGGTCTCGCAGCAGCTCACGCTGACCCCGCAACTGCAGCAGTCGATCCGGCTGCTGCA
ACTGTCCACCGTCGACCTGCAAACCGAAGTCGAGCGCTTCCTGCAGGACAACCCGCTGCTGGAACGGGGCGACGAGCCCG
TCGCCGACAGCGCCCCGGCCGAAACCACCGGCAGCGAGGCCGAGCCCGAACGCCAGGAACCGGAACGCGAGGACAACAGC
AGCGGCGAACTCACCGACTGGGGCAGCGGCAGCCGGCGCGGTGAAGACGGCGACGATTACGACCCGATGGCCAACGTGCC
GCGCCCGGTCACGCTGCGCGAACACCTGCTGGCGCAGCTGGGCGAAATCGCCCTGCCCGAGCGCGACTGCGCCATCGTGC
AGCTTCTGATCGAGGAACTGGACGAAGACGGCTACCTCACGATCGACCTGCAGGAACTGGCCGCCTCGCTGCCGCTGGAA
CTGGGCATCGAGGAAGACGAACTGGCGTTCGGTTTGAAACTGCTGCAGCAGTTCGAACCACAGGGGGTCGGCGCCCGCTC
GCTGTCCGAGTCGCTGGCGCTGCAGCTGGCCCAGCTGCCCGACAGCACCCCGGGCAAGGCCCTGGCGCACGCCATCGTCC
GCGAGCACCTGACCCTGCTCGGCAATCGCGACTACACCCGGCTCAAGCGTCTCCTCGACGTCAGCGACGCCGAACTGAAG
CAGGCCCAGTCCCTGATCGCCCGCCTCAACCCGCGCCCGGCCTCCGGTTTCGGCGTCGACGACACCCGCTACATCATCCC
GGATATCGCCGTGCGCCGCATCAAGGGGCGCTGGCGCGCCGAGCTCAACAACGCCGCCGTGCCGCGCCTGCGCGTCAACC
AGATGTACGCCCAGATGCTCAGCGAAAACCGCGGCAGCGGCGGTGAAATGAGCTCGCGCTTACAGGAGGCGAAATGGCTG
GTGAAGAATATTCAGCAACGATTTGACACCATCCTGAAAGTGGCGGAATCTATAGTCGAAAGACAGCAAGCGTTCTTTGA
ACACGGTGAAGTAGCCATGCGTCCACTCATCCTGCGCGACATTGCGGATGAGCTGGAACTACACGAATCCACGGTCTCCC
GTGTGACGACGCAGAAATACCTGCTGTGCCCGCGCGGCCTGTTCGAGCTGAAGTATTTCTTCGGCAGCGCCCTGGAAACG
GACAGCGGCGGCGAGTGCTCGGCCACCGCCATCAAGGCGCACATCCGCCAGATGATCGACGCCGAAAACCGCGCCAAACC
CCTCTCCGACAGCGCCCTGGCCGACGAGCTCGCAAAACAGGGCATACAGGTTGCAAGACGCACCGTAGCGAAGTATCGTG
AAGCCATGCAGATTCCACCGGTCAATCTGCGCAAGGTCCTCTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(105-296)

Sigma-54, DNA binding domain (Sigma54_DBD)

(310-465)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G2IYG9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

51.098

100

0.548

Multiple sequence alignment