Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   G4P54_RS01935 Genome accession   NZ_CP048852
Coordinates   355093..355539 (-) Length   148 a.a.
NCBI ID   WP_167871611.1    Uniprot ID   A0A6H0WHE5
Organism   Bacillus tequilensis strain EA-CB0015     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 350093..360539
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G4P54_RS01895 (G4P54_01890) - 350473..351609 (+) 1137 WP_167871609.1 zinc-dependent alcohol dehydrogenase -
  G4P54_RS01900 (G4P54_01895) - 351622..352077 (+) 456 WP_024715493.1 RDD family protein -
  G4P54_RS01905 (G4P54_01900) - 352159..352491 (+) 333 WP_167871610.1 YckD family protein -
  G4P54_RS01910 (G4P54_01905) - 352619..353041 (+) 423 WP_167873801.1 MarR family winged helix-turn-helix transcriptional regulator -
  G4P54_RS01915 (G4P54_01910) - 353029..353370 (+) 342 WP_024715490.1 DUF3147 family protein -
  G4P54_RS01920 (G4P54_01915) - 353390..354118 (+) 729 WP_024715489.1 AAA family ATPase -
  G4P54_RS01925 (G4P54_01920) - 354191..354599 (+) 409 Protein_333 family 1 glycosylhydrolase -
  G4P54_RS01930 (G4P54_01925) nin/comJ 354668..355066 (-) 399 WP_024715487.1 DNA-entry nuclease inhibitor Regulator
  G4P54_RS01935 (G4P54_01930) nucA/comI 355093..355539 (-) 447 WP_167871611.1 DNA-entry nuclease Machinery gene
  G4P54_RS01940 (G4P54_01935) tlpC 355713..357437 (-) 1725 WP_167871612.1 methyl-accepting chemotaxis protein TlpC -
  G4P54_RS01945 (G4P54_01940) hxlB 357550..358107 (-) 558 WP_167871613.1 6-phospho-3-hexuloisomerase -
  G4P54_RS01950 (G4P54_01945) hxlA 358113..358745 (-) 633 WP_167871614.1 3-hexulose-6-phosphate synthase -
  G4P54_RS01955 (G4P54_01950) hxlR 358974..359336 (+) 363 WP_024715482.1 transcriptional activator HxlR -

Sequence

Protein


Download         Length: 148 a.a.        Molecular weight: 16287.27 Da        Isoelectric Point: 4.4933

>NTDB_id=423533 G4P54_RS01935 WP_167871611.1 355093..355539(-) (nucA/comI) [Bacillus tequilensis strain EA-CB0015]
MNITMDIIKTILLVIVVIAAAAVGLIKGDFFPADQKTSQTKYDETIAFPSDRYPETAKHIKDAINEGHSDVCTIDRDGAE
EHREQSLKDVPPKTGYDRDEWPMAMCKEGGEGASVEYISPADNRGAGSWVGHQLTDYPDGTKVLFTIQ

Nucleotide


Download         Length: 447 bp        

>NTDB_id=423533 G4P54_RS01935 WP_167871611.1 355093..355539(-) (nucA/comI) [Bacillus tequilensis strain EA-CB0015]
GTGAACATCACGATGGACATCATAAAAACGATACTTCTCGTCATCGTCGTCATAGCAGCTGCAGCTGTTGGCCTGATAAA
AGGAGACTTTTTCCCAGCTGATCAAAAAACGTCTCAAACGAAATATGATGAAACAATTGCCTTCCCTTCTGACCGTTATC
CTGAAACGGCCAAACATATTAAGGATGCGATAAATGAAGGGCACTCAGATGTGTGCACCATCGACAGAGACGGAGCTGAG
GAACACCGCGAGCAATCATTAAAAGACGTACCGCCCAAAACGGGGTATGACAGAGATGAATGGCCAATGGCCATGTGCAA
AGAAGGCGGCGAAGGGGCTTCTGTAGAATATATTTCTCCCGCCGACAACCGCGGTGCAGGCTCTTGGGTCGGGCATCAGC
TTACAGATTATCCAGACGGTACAAAGGTTTTATTCACCATTCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(52-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6H0WHE5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

93.289

100

0.939