Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   G4P54_RS00580 Genome accession   NZ_CP048852
Coordinates   106033..107409 (+) Length   458 a.a.
NCBI ID   WP_024714028.1    Uniprot ID   A0A6H0WG44
Organism   Bacillus tequilensis strain EA-CB0015     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 101033..112409
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G4P54_RS00560 (G4P54_00560) ctsR 101383..101847 (+) 465 WP_167871486.1 transcriptional regulator CtsR -
  G4P54_RS00565 (G4P54_00565) mcsA 101861..102421 (+) 561 WP_024714025.1 protein-arginine kinase activator protein McsA -
  G4P54_RS00570 (G4P54_00570) - 102421..103512 (+) 1092 WP_167871487.1 protein arginine kinase -
  G4P54_RS00575 (G4P54_00575) clpC 103509..105941 (+) 2433 WP_024714027.1 ATP-dependent protease ATP-binding subunit ClpC Regulator
  G4P54_RS00580 (G4P54_00580) radA 106033..107409 (+) 1377 WP_024714028.1 DNA repair protein RadA Machinery gene
  G4P54_RS00585 (G4P54_00585) disA 107413..108495 (+) 1083 WP_024714029.1 DNA integrity scanning diadenylate cyclase DisA -
  G4P54_RS00590 (G4P54_00590) - 108613..109713 (+) 1101 WP_024714030.1 PIN/TRAM domain-containing protein -
  G4P54_RS00595 (G4P54_00595) ispD 109728..110426 (+) 699 WP_167871488.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  G4P54_RS00600 (G4P54_00600) ispF 110419..110895 (+) 477 WP_167871489.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 49469.91 Da        Isoelectric Point: 7.6838

>NTDB_id=423530 G4P54_RS00580 WP_024714028.1 106033..107409(+) (radA) [Bacillus tequilensis strain EA-CB0015]
MAKTKSKFICQSCGYESPKWMGKCPGCGAWNTMVEEMIKKAPANRRAAFSHSVQTVQKPSPITSIETSEEPRVKTQLGEF
NRVLGGGVVKGSLVLIGGDPGIGKSTLLLQVSAQLSDSSNSVLYISGEESVKQTKLRADRLGINNPSLHVLSETDMEYIS
SAIEEMNPAFVVVDSIQTVYQSDITSAPGSVSQVRECTAELMKIAKTKGVPIFIVGHVTKEGSIAGPRLLEHMVDTVLYF
EGERHHTFRILRAVKNRFGSTNEMGIFEMREEGLTEVLNPSEIFLEERSAGSAGSSITASMEGTRPILVEIQALISPTSF
GNPRRMATGIDHNRVSLLMAVLEKRVGLLLQNQDAYLKVAGGVKLDEPAIDLAIAISIASSFRDTPPNPADCFIGEVGLT
GEVRRVSRIEQRVKEAAKLGFKRMIIPGANLDGWTKPKGIEVIGVANVAEALRTSLGG

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=423530 G4P54_RS00580 WP_024714028.1 106033..107409(+) (radA) [Bacillus tequilensis strain EA-CB0015]
ATGGCTAAAACAAAATCGAAATTCATCTGCCAATCCTGCGGTTACGAGTCTCCAAAATGGATGGGGAAATGTCCGGGCTG
CGGTGCTTGGAATACAATGGTGGAAGAAATGATTAAAAAAGCGCCGGCCAATCGGAGAGCGGCTTTTTCTCATTCTGTTC
AAACTGTACAGAAACCTTCACCTATTACATCAATTGAAACATCAGAAGAACCCCGGGTGAAAACCCAGCTTGGCGAGTTT
AACAGAGTGCTTGGCGGAGGCGTCGTGAAAGGCTCACTCGTTTTAATCGGCGGCGATCCTGGTATCGGAAAGTCAACATT
ATTACTGCAGGTTTCCGCTCAATTATCAGATTCCTCAAACAGTGTCCTGTATATTTCAGGGGAAGAATCAGTGAAACAAA
CAAAACTGAGAGCAGACCGCCTCGGCATTAATAATCCGTCACTGCATGTTTTATCTGAAACCGATATGGAGTATATTTCG
TCTGCTATAGAAGAGATGAATCCAGCGTTTGTCGTTGTAGACTCTATTCAAACGGTTTACCAAAGCGATATCACATCAGC
TCCGGGCAGTGTATCACAGGTCAGAGAATGTACGGCTGAACTGATGAAGATTGCAAAAACAAAAGGAGTCCCGATTTTTA
TCGTAGGGCATGTGACGAAAGAAGGGTCTATTGCAGGGCCGAGACTGTTGGAGCATATGGTTGACACAGTTTTATATTTT
GAAGGGGAACGTCACCATACTTTCCGTATTTTACGGGCTGTGAAAAACCGTTTTGGTTCTACTAATGAAATGGGCATTTT
TGAAATGCGTGAAGAGGGGCTCACAGAGGTTTTGAATCCTTCAGAGATTTTCTTAGAAGAACGCTCAGCGGGATCCGCAG
GCTCAAGCATAACCGCTTCTATGGAAGGTACAAGACCGATTCTCGTAGAAATCCAGGCGCTCATCTCGCCAACAAGCTTC
GGCAATCCGCGGCGCATGGCAACGGGAATAGACCATAACAGAGTGTCACTGTTAATGGCTGTGTTAGAAAAAAGAGTGGG
GCTGCTGCTGCAAAACCAGGATGCTTATTTAAAAGTGGCTGGCGGCGTTAAGCTTGATGAACCGGCAATTGATCTTGCCA
TTGCGATCAGCATCGCATCAAGCTTTAGAGATACACCTCCAAATCCTGCAGATTGTTTTATAGGAGAAGTGGGATTAACC
GGAGAAGTCCGGCGGGTTTCAAGAATTGAACAGCGTGTGAAAGAAGCGGCTAAGCTTGGTTTTAAGCGCATGATCATACC
CGGGGCAAATCTGGATGGATGGACAAAACCAAAAGGGATTGAGGTTATCGGAGTAGCAAATGTTGCAGAGGCGCTTCGTA
CTTCATTAGGAGGATAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(72-222)

Subunit ChlI of Mg-chelatase (ChlI)

(365-430)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6H0WG44

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

98.69

100

0.987
  radA Streptococcus mitis NCTC 12261

63.355

98.908

0.627
  radA Streptococcus pneumoniae Rx1

63.355

98.908

0.627
  radA Streptococcus pneumoniae D39

63.355

98.908

0.627
  radA Streptococcus pneumoniae R6

63.355

98.908

0.627
  radA Streptococcus pneumoniae TIGR4

63.355

98.908

0.627
  radA Streptococcus mitis SK321

63.135

98.908

0.624