Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   G3580_RS02020 Genome accession   NZ_CP048836
Coordinates   452480..453877 (-) Length   465 a.a.
NCBI ID   WP_173763673.1    Uniprot ID   A0A6C1B0S9
Organism   Nitrogeniibacter mangrovi strain M9-3-2     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 447480..458877
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G3580_RS01985 (G3580_01980) - 447510..448259 (+) 750 WP_228720742.1 pteridine reductase -
  G3580_RS01990 (G3580_01985) ttcA 448315..449229 (+) 915 WP_407670984.1 tRNA 2-thiocytidine(32) synthetase TtcA -
  G3580_RS01995 (G3580_01990) - 449345..450214 (+) 870 WP_173763668.1 adenylate/guanylate cyclase domain-containing protein -
  G3580_RS02000 (G3580_01995) - 450269..450502 (-) 234 WP_173763669.1 PsiF family protein -
  G3580_RS02005 (G3580_02000) hprK 450624..451562 (-) 939 WP_173763670.1 HPr(Ser) kinase/phosphatase -
  G3580_RS02010 (G3580_02005) ptsN 451531..452016 (-) 486 WP_173763671.1 PTS IIA-like nitrogen regulatory protein PtsN -
  G3580_RS02015 (G3580_02010) hpf 452142..452465 (-) 324 WP_173763672.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  G3580_RS02020 (G3580_02015) rpoN/rpoN1 452480..453877 (-) 1398 WP_173763673.1 RNA polymerase factor sigma-54 Machinery gene
  G3580_RS02025 (G3580_02020) lptB 453893..454615 (-) 723 WP_173763674.1 LPS export ABC transporter ATP-binding protein -
  G3580_RS02030 (G3580_02025) recX 454602..455048 (-) 447 WP_173763675.1 recombination regulator RecX -
  G3580_RS02035 (G3580_02030) recA 455060..456103 (-) 1044 WP_173763676.1 recombinase RecA Machinery gene
  G3580_RS02040 (G3580_02035) - 456182..456667 (-) 486 WP_173763677.1 CinA family protein -
  G3580_RS02045 (G3580_02040) - 456677..457159 (-) 483 WP_173763678.1 phosphatidylglycerophosphatase A -
  G3580_RS02050 (G3580_02045) thiL 457212..458180 (-) 969 WP_173763679.1 thiamine-phosphate kinase -

Sequence

Protein


Download         Length: 465 a.a.        Molecular weight: 52254.07 Da        Isoelectric Point: 4.8904

>NTDB_id=423435 G3580_RS02020 WP_173763673.1 452480..453877(-) (rpoN/rpoN1) [Nitrogeniibacter mangrovi strain M9-3-2]
MKPTLQLKLSQHLTLTPQLQQSIKLLQLSTIELNQEIEKALLDNPLLEREDDGGPGEQDSATDTASNESEGGSEALEAMS
EWASVGSGSTTNRDDDEDSDFQSFHAAQVSLREHLDQQIALTPMSDRDRTLVRILIEALDDDGYLTQSLEDMLELFDAEN
EVELEDLQIALHHLQHLDPPGVGARNPSECLALQLRALPQGDTRDLAMRIVTEHLELLAQRDFVRLRRNLQCTEDALRDA
HHLICSLSPRPCAPFALNDTRYVIPDVVVRKLRGRWTAMLNPEAMPRLKVNALYAQLLQQNRGNNGDLGGQLQEAKWLIK
NVQQRFDTILRVAQAIVDQQRQFFDYGEVAMRPLTLREIAEELELHESTISRVTTQKYMSTPRGVLEFKYFFGSHVATDT
GGAASSTAIRALIRQLVDAEDRKKPLSDSKIADLLGQQGMVVARRTVAKYRESLNIPPVSKRKTI

Nucleotide


Download         Length: 1398 bp        

>NTDB_id=423435 G3580_RS02020 WP_173763673.1 452480..453877(-) (rpoN/rpoN1) [Nitrogeniibacter mangrovi strain M9-3-2]
ATGAAACCCACACTTCAACTCAAACTCTCTCAGCACCTGACGCTGACACCGCAACTGCAACAGTCCATCAAGCTGCTGCA
GCTGTCGACCATCGAGTTGAACCAGGAGATCGAAAAGGCTCTGCTCGACAATCCGCTGCTCGAACGCGAGGACGACGGCG
GCCCCGGCGAGCAGGACAGCGCCACCGACACCGCGAGCAACGAGTCCGAGGGGGGCAGTGAAGCCCTCGAGGCGATGTCC
GAGTGGGCCAGCGTCGGCTCCGGCAGCACCACCAACCGGGACGACGACGAGGACAGCGACTTCCAGTCCTTTCACGCCGC
CCAGGTGTCCCTGCGGGAACACCTGGACCAGCAGATCGCCCTGACCCCCATGTCCGACCGCGATCGAACCCTGGTGCGCA
TCCTCATCGAAGCGCTCGACGACGACGGCTATTTGACCCAATCGCTCGAGGACATGCTGGAGCTGTTCGACGCCGAGAAC
GAAGTCGAACTGGAGGACCTGCAGATCGCCCTGCATCACCTCCAACACCTCGACCCGCCGGGCGTCGGCGCGCGCAATCC
GTCCGAGTGCCTGGCCCTGCAGCTGCGTGCGTTGCCGCAAGGCGACACCCGCGACCTGGCGATGCGCATCGTCACCGAGC
ACCTGGAACTGCTCGCACAACGCGACTTCGTGCGCCTGCGGCGCAACCTGCAGTGCACCGAAGACGCGCTGCGCGACGCC
CATCACCTCATCTGCAGCCTCAGCCCGCGCCCCTGCGCACCGTTCGCGCTCAACGACACCCGCTATGTCATCCCGGACGT
GGTGGTGCGCAAACTGCGCGGTCGCTGGACCGCCATGCTCAACCCCGAAGCGATGCCGCGCCTGAAGGTCAATGCCCTGT
ATGCGCAGCTGCTGCAGCAGAACCGGGGGAACAACGGCGACCTGGGCGGTCAGCTGCAGGAAGCCAAATGGCTCATCAAG
AACGTCCAGCAGCGCTTCGACACCATCCTGCGGGTGGCGCAGGCCATCGTTGACCAACAGCGGCAGTTCTTCGATTATGG
AGAGGTGGCGATGCGCCCGCTCACGCTCCGGGAAATCGCCGAAGAGCTGGAGCTGCACGAATCGACCATTTCGCGCGTCA
CCACGCAAAAGTACATGTCTACGCCGCGCGGCGTACTGGAGTTCAAGTATTTCTTCGGCAGTCATGTGGCCACCGACACC
GGCGGGGCAGCTTCGTCCACCGCCATTCGCGCCCTGATCCGCCAGCTGGTGGACGCCGAAGACCGCAAGAAACCCCTATC
CGATTCCAAAATCGCAGACTTACTTGGCCAGCAGGGCATGGTTGTGGCCCGACGCACGGTCGCCAAATACCGTGAGTCCC
TCAACATCCCGCCGGTCAGCAAACGCAAGACGATCTGA

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(311-463)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(101-294)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6C1B0S9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

53.892

100

0.581