Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   G3257_RS03640 Genome accession   NZ_CP048832
Coordinates   826361..827839 (+) Length   492 a.a.
NCBI ID   WP_034778385.1    Uniprot ID   -
Organism   Janthinobacterium lividum strain EIF1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 821361..832839
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G3257_RS03615 (G3257_03610) - 822797..823807 (+) 1011 WP_151092312.1 KpsF/GutQ family sugar-phosphate isomerase -
  G3257_RS03620 (G3257_03615) - 823807..824340 (+) 534 WP_034778379.1 HAD hydrolase family protein -
  G3257_RS03625 (G3257_03620) lptC 824383..824994 (+) 612 WP_034778380.1 LPS export ABC transporter periplasmic protein LptC -
  G3257_RS03630 (G3257_03625) lptA 825020..825601 (+) 582 WP_034778381.1 lipopolysaccharide transport periplasmic protein LptA -
  G3257_RS03635 (G3257_03630) lptB 825601..826356 (+) 756 WP_034778383.1 LPS export ABC transporter ATP-binding protein -
  G3257_RS03640 (G3257_03635) rpoN/rpoN1 826361..827839 (+) 1479 WP_034778385.1 RNA polymerase factor sigma-54 Machinery gene
  G3257_RS03645 (G3257_03640) raiA 827909..828271 (+) 363 WP_034778388.1 ribosome-associated translation inhibitor RaiA -
  G3257_RS03650 (G3257_03645) - 828407..829492 (+) 1086 WP_034778390.1 enoyl-CoA hydratase/isomerase family protein -
  G3257_RS03655 (G3257_03650) rapZ 829493..830356 (-) 864 WP_035824128.1 RNase adapter RapZ -
  G3257_RS03660 (G3257_03655) hprK 830572..831507 (-) 936 WP_034753337.1 HPr(Ser) kinase/phosphatase -
  G3257_RS03665 (G3257_03660) - 831547..832029 (-) 483 WP_034753339.1 PTS sugar transporter subunit IIA -

Sequence

Protein


Download         Length: 492 a.a.        Molecular weight: 54595.31 Da        Isoelectric Point: 5.1050

>NTDB_id=423370 G3257_RS03640 WP_034778385.1 826361..827839(+) (rpoN/rpoN1) [Janthinobacterium lividum strain EIF1]
MKQSLQLRTSQHLALTPQLQQSIRLLQLSTLELHQELEQLLTDNPLLERLDDPLDRSLRLLSDGALSSTAAPAEAPPQPP
GQEVPAAPAEAETFDGDAGEGPAAADGGDSDWSEASRGKAPDDEDSRPQLEAHHCTLREHLMEQMRVTVLELRDRALVEL
IIDALDDNGYLEESLDDILARLPEELEIDVDEMRTALSLLQSFDPPGVGARNASECLALQIKRLPHIAMVTRRMALVIVE
KHLAWFAQRDFNKLKKALDCDDEDLREAQTVIRQCNPHPGAVFASDVSDYVVPDVVVKRARNGWQVTLNNDVMPRLRVNA
MYANLLKQGKGEGAMGAQLQEAKWLIKNMRQRFDTILRVAQAIVERQKNFFSHGAVAMRPLVLREIADTLGLHESTISRV
TTQKYMLTPHGMFELKYFFGSHVATEAGGEASSTAIRALIVQLTGAEDPKNPLSDSKIADMLGEQGMVIARRTVAKYREA
LKIPPVSLRKCL

Nucleotide


Download         Length: 1479 bp        

>NTDB_id=423370 G3257_RS03640 WP_034778385.1 826361..827839(+) (rpoN/rpoN1) [Janthinobacterium lividum strain EIF1]
ATGAAACAATCATTGCAGCTGCGCACTTCGCAGCACCTGGCACTGACGCCGCAGTTGCAGCAATCGATACGCCTGTTGCA
ATTGTCTACCCTGGAATTGCATCAGGAATTAGAGCAATTGCTGACGGACAATCCCTTGCTCGAACGCCTCGACGATCCGC
TCGACCGTTCGTTGCGCCTGCTGTCCGACGGCGCCTTGAGTTCCACGGCCGCGCCGGCCGAAGCGCCGCCCCAGCCGCCA
GGCCAGGAGGTGCCCGCAGCGCCGGCCGAAGCGGAAACCTTTGATGGCGATGCCGGCGAGGGTCCGGCCGCCGCGGATGG
CGGCGACAGCGACTGGAGCGAGGCGAGCCGGGGCAAGGCTCCCGACGACGAAGATTCGCGTCCGCAACTGGAGGCCCATC
ACTGCACCCTGCGCGAACACCTGATGGAGCAGATGCGAGTGACGGTGCTCGAATTGCGCGACCGCGCGCTGGTCGAGCTG
ATCATCGACGCGCTCGACGACAATGGCTACCTGGAAGAGTCGCTCGACGACATCCTGGCGCGCCTGCCGGAAGAGCTGGA
AATCGACGTCGACGAGATGCGCACGGCCTTGTCTCTCTTGCAAAGTTTCGATCCGCCCGGCGTGGGCGCGCGCAATGCGT
CCGAATGCCTGGCGCTGCAAATCAAGCGCTTGCCGCATATCGCCATGGTGACGCGCCGCATGGCCCTCGTCATCGTGGAA
AAGCACCTGGCCTGGTTTGCCCAGCGCGATTTCAACAAGCTGAAAAAAGCCCTCGATTGCGACGATGAAGACTTGCGCGA
AGCGCAGACGGTGATTCGCCAGTGCAATCCGCATCCGGGCGCCGTGTTCGCCTCGGACGTGTCCGATTACGTGGTGCCCG
ACGTCGTCGTCAAGCGCGCGCGCAATGGCTGGCAAGTGACCTTGAACAACGATGTCATGCCGCGCCTGCGCGTCAATGCC
ATGTACGCCAACTTGCTCAAGCAGGGCAAGGGCGAGGGCGCCATGGGCGCACAGTTGCAGGAAGCCAAGTGGCTGATCAA
GAATATGCGCCAGCGCTTCGACACGATCCTGCGCGTGGCGCAGGCGATAGTAGAAAGACAAAAGAACTTTTTCTCGCATG
GGGCCGTTGCCATGCGTCCCCTTGTGCTCCGTGAAATAGCTGATACACTGGGTTTACACGAGAGTACTATCTCGCGGGTA
ACAACTCAAAAGTACATGCTGACCCCGCACGGCATGTTCGAGTTGAAATATTTCTTTGGTAGCCACGTCGCCACCGAAGC
GGGGGGCGAAGCGTCATCGACGGCGATACGGGCATTGATCGTGCAACTGACAGGAGCAGAAGACCCTAAAAATCCTTTAT
CCGACAGTAAGATTGCGGACATGCTGGGGGAACAAGGCATGGTGATTGCGCGACGTACTGTTGCCAAATACCGGGAAGCG
TTGAAAATTCCGCCAGTGAGCCTGCGTAAGTGTTTGTAA

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(131-322)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(337-490)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

60.707

100

0.628

Multiple sequence alignment