Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilS   Type   Regulator
Locus tag   GN234_RS22760 Genome accession   NZ_CP048810
Coordinates   5173934..5175523 (-) Length   529 a.a.
NCBI ID   WP_176689116.1    Uniprot ID   A0A6N1CJK3
Organism   Pseudomonas bijieensis strain L22-9     
Function   regulate pilin expression (predicted from homology)   
Competence regulation

Genomic Context

Location: 5168934..5180523
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GN234_RS22740 (GN234_22665) - 5169251..5169652 (+) 402 WP_134925875.1 type IV pilin protein -
  GN234_RS22745 (GN234_22670) thiO 5169709..5170809 (+) 1101 WP_176689114.1 glycine oxidase ThiO -
  GN234_RS22750 (GN234_22675) - 5172051..5172416 (+) 366 WP_176689115.1 DUF6124 family protein -
  GN234_RS22755 (GN234_22680) pilR 5172591..5173937 (-) 1347 WP_163856580.1 sigma-54-dependent transcriptional regulator Regulator
  GN234_RS22760 (GN234_22685) pilS 5173934..5175523 (-) 1590 WP_176689116.1 two-component system sensor histidine kinase NtrB Regulator
  GN234_RS22765 (GN234_22690) - 5175513..5175746 (-) 234 WP_109756371.1 PP0621 family protein -
  GN234_RS22770 (GN234_22695) - 5175872..5176888 (-) 1017 WP_176689117.1 outer membrane protein assembly factor BamD -
  GN234_RS22775 (GN234_22700) rluD 5177040..5178002 (+) 963 WP_116833478.1 23S rRNA pseudouridine(1911/1915/1917) synthase RluD -
  GN234_RS22780 (GN234_22705) pgeF 5177999..5178724 (+) 726 WP_163856586.1 peptidoglycan editing factor PgeF -

Sequence

Protein


Download         Length: 529 a.a.        Molecular weight: 58937.72 Da        Isoelectric Point: 7.2072

>NTDB_id=423169 GN234_RS22760 WP_176689116.1 5173934..5175523(-) (pilS) [Pseudomonas bijieensis strain L22-9]
MSAETSSPREKQTQRLLRLYHLYRLSIGITLVLLISSKLDNRLLEFANDDLLRSGSWLYLVLNILLVVFLENTRRPARLF
GLALTDVLLLSWLFFAAGGVPSAIGNLLIVSVAIGNTLLRGRVGLLIAAVATIGIVGSTFFLGLSDSNRPSSYLQAGTLG
ALCFAAALLVQGLTRRLEASETLAEQRASEVIGLEALNALILQRMRTGILVLDRERRVQLANESALNLLGMHDLVGQRID
DYSVALVERLRLWLNNPSLRAQSLTISGTGLALQPSFIALGHNEQHQILVFLEDLAQVAQQAQQLKLASLGRLTAGIAHE
IRNPLGAISHAAQLLRESEELNDADRRLTQIIQDHSQRMNRVIENVLQLSRRQQTTPQRLDLRTWLEQFVRQAHESMAEH
QQLHLSIDPGDYTTLMDPDQLTQVLDNLLRNAWRHSAMAHEQAEVWLKLFIDPHSQLSTLDIIDNGPGVTPEQQAHLFEP
FFTTSSQGTGLGLYLSRELCESNQARLDFKPRQGGGCFRITFAHGRKQI

Nucleotide


Download         Length: 1590 bp        

>NTDB_id=423169 GN234_RS22760 WP_176689116.1 5173934..5175523(-) (pilS) [Pseudomonas bijieensis strain L22-9]
GTGAGCGCTGAGACCTCAAGCCCGCGCGAAAAACAGACGCAGCGTCTGCTGCGTCTCTATCACCTGTACCGCCTGAGCAT
CGGCATCACCCTGGTGCTGCTGATCTCCAGCAAACTGGACAATCGCCTGCTGGAGTTCGCCAACGACGACCTGCTGCGCA
GCGGCAGTTGGTTGTATCTGGTGCTGAACATCCTGCTTGTAGTGTTCCTTGAAAACACTCGCCGCCCTGCCCGGCTGTTC
GGCCTGGCCCTCACCGACGTACTGCTGCTTTCGTGGCTGTTCTTCGCCGCAGGCGGTGTGCCCAGCGCCATCGGCAACCT
GCTCATCGTCTCGGTAGCCATCGGCAATACGCTATTGCGAGGCCGAGTCGGCCTGTTGATCGCCGCTGTCGCCACGATCG
GTATCGTCGGCTCGACTTTCTTCCTCGGCCTGAGCGACTCGAACCGCCCCAGCAGCTACCTGCAAGCCGGGACCCTGGGG
GCACTGTGCTTCGCCGCCGCCCTGCTGGTACAAGGCCTGACCCGACGACTGGAAGCCAGCGAAACCCTGGCTGAGCAACG
GGCCAGCGAAGTGATCGGCCTCGAAGCACTCAACGCGCTGATCCTGCAACGCATGCGCACCGGCATCCTGGTGCTGGACC
GCGAACGTCGGGTACAACTGGCCAACGAAAGCGCCTTGAACCTGCTGGGCATGCACGACCTGGTCGGCCAGCGGATCGAT
GACTACTCCGTCGCCCTGGTCGAACGTCTGCGACTGTGGCTGAACAACCCTAGCCTGCGCGCACAGAGCCTGACCATCAG
CGGCACAGGCCTGGCACTGCAACCGAGCTTCATCGCCCTGGGCCACAACGAGCAACATCAGATCCTGGTCTTCCTCGAAG
ACCTGGCCCAGGTTGCGCAGCAGGCCCAACAACTCAAGCTAGCCTCCCTCGGACGCCTGACGGCCGGCATCGCCCACGAA
ATCCGCAACCCCCTGGGCGCCATCAGCCACGCGGCGCAATTGCTGCGCGAATCCGAGGAACTGAACGACGCGGATCGGCG
TCTGACGCAGATTATTCAAGATCACTCTCAACGAATGAATCGCGTCATTGAAAACGTCCTGCAACTGTCTCGCCGCCAGC
AAACCACACCCCAACGCCTCGATCTGCGGACCTGGCTCGAACAGTTCGTCCGACAGGCCCACGAAAGCATGGCCGAGCAC
CAGCAATTGCACCTGAGCATCGATCCGGGCGACTACACCACGCTGATGGACCCCGACCAGTTGACCCAGGTACTCGACAA
CCTGCTGCGCAACGCCTGGCGCCACAGCGCAATGGCCCACGAACAGGCCGAGGTCTGGCTGAAACTGTTCATCGACCCCC
ACAGCCAGCTGTCCACACTGGACATCATCGACAACGGCCCCGGCGTGACTCCAGAACAGCAAGCGCACTTGTTCGAGCCC
TTCTTTACCACCAGTAGCCAGGGCACCGGCCTTGGGCTCTATCTGTCCCGTGAGCTGTGCGAAAGCAACCAGGCCCGCCT
AGACTTCAAACCACGCCAAGGCGGCGGCTGCTTTCGCATCACCTTTGCTCACGGACGGAAACAGATTTGA

Domains

Predicted by InterproScan.

His Kinase A (phospho-acceptor) domain (HisKA)

(309-374)

Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (HATPase_c)

(417-522)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6N1CJK3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilS Pseudomonas aeruginosa PAK

59.659

99.811

0.595

Multiple sequence alignment