Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   PARA_RS00180 Genome accession   NC_015964
Coordinates   32173..32715 (-) Length   180 a.a.
NCBI ID   WP_014063999.1    Uniprot ID   A0AB33QI98
Organism   Haemophilus parainfluenzae T3T1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 27173..37715
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PARA_RS00155 (PARA_00330) - 27532..27975 (-) 444 WP_014063995.1 methyltransferase family protein -
  PARA_RS00160 (PARA_00340) - 28027..28641 (-) 615 WP_014063996.1 YfgM family protein -
  PARA_RS00165 (PARA_00350) hisS 28660..29931 (-) 1272 WP_014063997.1 histidine--tRNA ligase -
  PARA_RS00170 (PARA_00360) ispG 29941..31047 (-) 1107 WP_005695590.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  PARA_RS00175 (PARA_00370) - 31066..32097 (-) 1032 WP_014063998.1 RodZ domain-containing protein -
  PARA_RS00180 (PARA_00380) pilF/pilF2 32173..32715 (-) 543 WP_014063999.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  PARA_RS00185 (PARA_00390) - 32785..33933 (-) 1149 WP_005698372.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  PARA_RS00190 (PARA_00400) - 34235..35110 (+) 876 WP_014064000.1 D-alanyl-D-alanine carboxypeptidase family protein -
  PARA_RS00195 (PARA_00410) - 35265..36146 (+) 882 WP_014064001.1 metal ABC transporter substrate-binding protein -
  PARA_RS00200 (PARA_00420) - 36146..37042 (+) 897 WP_014064002.1 manganese/iron ABC transporter ATP-binding protein -

Sequence

Protein


Download         Length: 180 a.a.        Molecular weight: 20466.16 Da        Isoelectric Point: 6.0918

>NTDB_id=42221 PARA_RS00180 WP_014063999.1 32173..32715(-) (pilF/pilF2) [Haemophilus parainfluenzae T3T1]
MKLIPINILSAVIFPFVFSACISQSSVDFNKQQAAKARVELALGYLQQEDFVQAKLNLDKAFEHDERYYLVHSALAHFYQ
LQGDTEKAKQAYLQAIKLDDKQGDVYNNFGAFLCGQGEFEQAYSQFNAALAAPNYYHQADTYENMALCAFVGKQTDVYQQ
ALEKLRQVDPSRAEKLRTLK

Nucleotide


Download         Length: 543 bp        

>NTDB_id=42221 PARA_RS00180 WP_014063999.1 32173..32715(-) (pilF/pilF2) [Haemophilus parainfluenzae T3T1]
ATGAAATTAATCCCAATTAACATTCTAAGTGCGGTCATTTTTCCTTTTGTTTTTTCTGCTTGCATTTCTCAGTCTTCAGT
CGATTTTAATAAACAACAAGCAGCCAAAGCGCGCGTTGAATTGGCTTTAGGCTATTTGCAACAAGAGGATTTTGTTCAAG
CAAAACTTAATTTAGATAAAGCCTTTGAGCATGATGAGCGGTATTATCTTGTGCACTCGGCACTTGCGCATTTTTATCAA
TTACAAGGCGATACAGAAAAAGCGAAGCAAGCTTATTTACAGGCGATTAAGTTAGACGATAAGCAAGGCGACGTGTACAA
CAACTTCGGTGCTTTTTTATGTGGGCAAGGTGAGTTTGAACAAGCTTATTCGCAATTTAATGCGGCACTCGCTGCGCCGA
ATTATTATCATCAAGCCGATACCTACGAAAACATGGCACTTTGTGCTTTTGTCGGAAAACAAACCGATGTTTATCAACAA
GCGTTGGAGAAATTGCGCCAAGTTGATCCTTCTAGAGCGGAAAAGCTCCGCACGCTCAAATAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(74-101)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

64.535

95.556

0.617
  pilF Glaesserella parasuis strain SC1401

49.153

98.333

0.483

Multiple sequence alignment