Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   G0027_RS09920 Genome accession   NZ_CP048654
Coordinates   2064922..2065641 (+) Length   239 a.a.
NCBI ID   WP_005180513.1    Uniprot ID   N9KYM2
Organism   Acinetobacter indicus strain C15_T     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2059922..2070641
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G0027_RS09900 (G0027_09875) - 2061351..2061965 (+) 615 WP_194088129.1 IS607 family transposase -
  G0027_RS09905 (G0027_09880) - 2061962..2063119 (+) 1158 WP_194088130.1 RNA-guided endonuclease TnpB family protein -
  G0027_RS09910 (G0027_09885) pilM 2063268..2064290 (+) 1023 WP_160232596.1 pilus assembly protein PilM Machinery gene
  G0027_RS09915 (G0027_09890) pilN 2064290..2064925 (+) 636 WP_005180516.1 PilN domain-containing protein Machinery gene
  G0027_RS09920 (G0027_09895) pilO 2064922..2065641 (+) 720 WP_005180513.1 type 4a pilus biogenesis protein PilO Machinery gene
  G0027_RS09925 (G0027_09900) pilP 2065641..2066168 (+) 528 WP_045795346.1 pilus assembly protein PilP Machinery gene
  G0027_RS09930 (G0027_09905) pilQ 2066229..2068367 (+) 2139 Protein_1922 type IV pilus secretin PilQ -
  G0027_RS09935 (G0027_09910) aroK 2068404..2068946 (+) 543 WP_075167858.1 shikimate kinase AroK -
  G0027_RS09940 (G0027_09915) aroB 2068969..2070051 (+) 1083 WP_180192315.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 239 a.a.        Molecular weight: 27087.71 Da        Isoelectric Point: 5.1311

>NTDB_id=422131 G0027_RS09920 WP_005180513.1 2064922..2065641(+) (pilO) [Acinetobacter indicus strain C15_T]
MSLDQYEEMNQDPVAAPKKKMTLDKFLQQWNTLDPNNYGSWPFSVKLTIWIFIIILIAFLGYFLAIKPKLDAISNARAQE
QNLLNEFREKDSKLRNLQQYQAQLQEMEANFNQQLQQLPKETEIPGLVEDVNVTGVNSGLRFKNIRLENEIKQEFFIEQP
ISIEATGDYHAFGSFVSGTAALPRIVTMHDFTITAGENKEKKTDIPQITYVAKAKTYRYVGNTDAQAGNSTTPATGAAQ

Nucleotide


Download         Length: 720 bp        

>NTDB_id=422131 G0027_RS09920 WP_005180513.1 2064922..2065641(+) (pilO) [Acinetobacter indicus strain C15_T]
ATGAGTCTCGATCAATATGAAGAAATGAATCAGGATCCTGTTGCTGCGCCAAAGAAAAAAATGACGCTGGATAAATTCCT
GCAGCAATGGAATACCTTAGATCCGAATAACTATGGCAGCTGGCCGTTTTCCGTCAAGCTGACCATCTGGATCTTTATCA
TTATTTTAATTGCCTTCCTGGGCTATTTCCTGGCCATCAAGCCGAAGCTGGATGCAATTTCCAATGCGCGTGCACAAGAG
CAGAACCTGCTGAATGAGTTTCGTGAAAAAGATTCCAAACTGCGTAACTTGCAGCAATATCAGGCGCAGTTACAGGAAAT
GGAAGCCAACTTTAACCAGCAGCTACAACAGTTGCCAAAAGAAACCGAAATTCCAGGTCTGGTTGAAGATGTGAACGTTA
CGGGTGTGAACTCAGGCCTAAGATTTAAAAATATCCGTCTGGAAAATGAAATCAAGCAAGAGTTCTTTATCGAGCAGCCA
ATTTCGATTGAAGCCACCGGTGATTATCACGCTTTTGGTTCTTTTGTGAGTGGTACAGCTGCTTTACCGCGAATTGTGAC
CATGCATGATTTCACTATTACTGCTGGTGAAAACAAGGAAAAGAAAACCGATATTCCACAAATTACCTATGTCGCGAAAG
CCAAGACCTATCGCTATGTCGGGAATACCGATGCACAGGCCGGTAATAGTACGACTCCAGCCACAGGAGCAGCACAATGA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(74-220)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB N9KYM2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Acinetobacter baumannii D1279779

69.748

99.582

0.695
  comO Acinetobacter baylyi ADP1

67.213

100

0.686

Multiple sequence alignment