Detailed information    

insolico Bioinformatically predicted

Overview

Name   comB4   Type   Machinery gene
Locus tag   G3M67_RS01025 Genome accession   NZ_CP048601
Coordinates   219070..221433 (-) Length   787 a.a.
NCBI ID   WP_163524303.1    Uniprot ID   -
Organism   Helicobacter pylori strain GCT 27     
Function   transformation-associated type IV transport system (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 214070..226433
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G3M67_RS01000 (G3M67_01000) lpxE 214433..215032 (+) 600 WP_163524301.1 lipid A 1-phosphatase LpxE -
  G3M67_RS01005 (G3M67_01005) nspC 215139..216356 (-) 1218 WP_163524302.1 carboxynorspermidine decarboxylase -
  G3M67_RS01010 (G3M67_01010) cheV1 216353..217318 (-) 966 WP_000785454.1 chemotaxis protein CheV1 -
  G3M67_RS01015 (G3M67_01015) - 217425..218765 (-) 1341 WP_001938079.1 COG3014 family protein -
  G3M67_RS01025 (G3M67_01025) comB4 219070..221433 (-) 2364 WP_163524303.1 VirB4 family type IV secretion/conjugal transfer ATPase Machinery gene
  G3M67_RS01030 (G3M67_01030) comB3 221435..221698 (-) 264 WP_000584973.1 hypothetical protein Machinery gene
  G3M67_RS01035 (G3M67_01035) comB2 221698..221979 (-) 282 WP_001272690.1 TrbC/VirB2 family protein Machinery gene
  G3M67_RS01040 (G3M67_01040) - 222125..222952 (-) 828 WP_001154904.1 DUF5718 family protein -
  G3M67_RS01045 (G3M67_01045) - 223045..224097 (-) 1053 WP_163524304.1 MnmA/TRMU family protein -
  G3M67_RS01050 (G3M67_01050) dnaG 224094..225773 (-) 1680 WP_163524305.1 DNA primase -
  G3M67_RS01055 (G3M67_01055) groES 226061..226417 (+) 357 WP_000671951.1 co-chaperone GroES -

Sequence

Protein


Download         Length: 787 a.a.        Molecular weight: 90243.50 Da        Isoelectric Point: 7.7036

>NTDB_id=421703 G3M67_RS01025 WP_163524303.1 219070..221433(-) (comB4) [Helicobacter pylori strain GCT 27]
MLEKFLGTIKQKVSNYFLGALPKSYSMSEENNILGLYDEHFLLTKNENLVGILRLEGVSYTHLSTEQLQDLFTERQMALD
SLEKVVARLVVKRRKIDHQQNIQSDSQYLQAILNQFENKEVYENQYFLVLESTHSLQGVLEHKKKSFMHANRENFKDILS
YKAHFLQETLKSLEIQLKNYAPKLLNSKEVLNFYAEYINGFDLPLKPLVGGYLSDSYIASSITFEKDYFIQESFNQKTYN
RLIGVKAYESERITSIAIGALLYQETPLDIIFSIEPMSAHKTLSFLKERAKFSMSNLVKNELLEYQELVKTKRLSMQKFA
LNVLIKAPSLEDLDAQTSLVLGLLFKENLVGVIETFGLKGGYFSFFPERIHLNHRLRFLTSKALACLMVFERQNLGFKAN
SWGNSPLSVFKNLDYSPFLFNFHNQEVSHNNAKEIARVNGHTLIIGATGSGKSTLISFLMMSTLRYQNMRLLAFDRMQGL
YSFTEFFKGHYHDGQSFSINPFCLEPNLQNLEFLQSFFLSMFDLAPSRDKEALEDMNAISGAIKSLYETLYPKDFSLLDF
KETLKRTSSNQLGLSLEPYLNNPLFNALNDAFNSSAFLNVINLDAITQNPKDLGLLAYYLFYKILEESRKNDSGFLVFLD
EFKSYVENDLLNTKINALITQARKANGVVVLALQDIYQLSGVKNAHSFLSNMGTLILYPQKNARELKHNFNVPLSETEIS
FLENTPLYARQVLVKNLGNGSSNMIDVSLEGLGRYLKIFNSDSSHVNKVKALQKDYPTEWREKLLKS

Nucleotide


Download         Length: 2364 bp        

>NTDB_id=421703 G3M67_RS01025 WP_163524303.1 219070..221433(-) (comB4) [Helicobacter pylori strain GCT 27]
ATGCTAGAAAAGTTTTTAGGCACTATCAAGCAAAAAGTTTCAAATTATTTTTTAGGGGCTTTGCCTAAAAGCTATTCAAT
GAGTGAAGAAAACAACATTTTAGGCTTGTATGATGAGCATTTTTTACTCACTAAAAACGAAAACTTAGTGGGCATCCTCC
GTTTAGAGGGGGTGAGTTACACCCATTTAAGCACAGAACAATTGCAAGATCTTTTCACCGAGCGCCAAATGGCGTTGGAT
TCTTTGGAAAAAGTCGTGGCGCGCCTCGTGGTTAAAAGGCGTAAAATTGATCACCAACAAAACATTCAATCTGATTCTCA
ATACTTGCAAGCGATCTTGAATCAATTTGAAAACAAAGAAGTGTATGAAAACCAGTATTTTTTAGTTTTAGAAAGTACGC
ACTCTTTACAGGGCGTTTTAGAGCATAAGAAAAAATCTTTCATGCATGCCAATAGGGAAAATTTTAAGGATATTCTCTCT
TATAAAGCGCATTTTTTACAAGAAACTTTAAAAAGCTTAGAAATCCAGCTCAAAAACTATGCCCCCAAACTCTTAAACTC
TAAAGAGGTTTTGAATTTTTATGCAGAATACATTAATGGGTTTGATCTCCCTTTAAAACCCCTAGTAGGGGGGTATTTGA
GCGATAGCTATATCGCTAGTTCTATCACTTTTGAAAAAGATTATTTCATTCAAGAAAGCTTTAACCAAAAAACCTACAAC
CGCTTGATTGGCGTTAAAGCTTATGAGAGCGAGCGCATCACTTCTATAGCGATCGGAGCGCTTTTATACCAAGAGACGCC
TTTGGATATTATCTTTTCCATAGAGCCAATGAGTGCGCATAAAACGCTGAGTTTTTTAAAAGAGAGAGCCAAGTTTAGCA
TGTCCAATCTCGTTAAAAACGAGCTGCTAGAATACCAGGAATTAGTCAAAACCAAACGCTTATCCATGCAAAAATTCGCC
CTAAACGTTCTTATCAAAGCCCCCAGTTTAGAGGATTTAGACGCTCAAACGAGCTTAGTTTTAGGGCTTTTATTTAAAGA
AAACTTAGTGGGCGTTATAGAAACTTTTGGCTTGAAAGGGGGGTATTTTTCTTTTTTCCCTGAACGCATCCATTTAAACC
ACCGCTTGCGTTTTTTAACTTCTAAAGCCCTAGCGTGTTTGATGGTGTTTGAAAGGCAAAATTTAGGCTTTAAGGCTAAT
TCATGGGGGAATAGCCCTTTGAGCGTGTTTAAAAATTTGGATTATTCCCCTTTTTTATTCAATTTCCACAACCAAGAAGT
GAGCCACAATAACGCTAAAGAAATCGCCAGAGTGAATGGGCATACTCTAATCATAGGGGCTACCGGAAGCGGTAAAAGCA
CGCTGATTAGCTTTTTAATGATGAGCACTTTGAGATACCAAAACATGCGCCTTTTAGCTTTTGACAGGATGCAAGGGCTG
TATTCTTTCACCGAATTTTTTAAAGGGCATTACCATGACGGCCAATCTTTTAGTATTAACCCCTTTTGTTTAGAGCCTAA
TTTACAGAATTTAGAATTTTTGCAATCCTTTTTTTTGAGCATGTTTGATCTCGCCCCTTCAAGGGATAAAGAAGCCTTAG
AAGACATGAATGCGATTTCTGGTGCGATTAAGAGCCTTTATGAGACCTTATACCCTAAAGATTTTAGTTTGTTGGATTTT
AAAGAAACGCTTAAAAGAACCTCATCTAACCAATTGGGCTTGAGCTTGGAGCCGTATTTGAATAACCCCCTTTTTAACGC
TTTGAATGACGCGTTCAATTCCAGTGCTTTTTTAAACGTGATCAACTTAGACGCTATCACCCAAAACCCTAAAGACTTAG
GGCTTTTAGCCTATTACTTGTTTTATAAGATCTTAGAAGAGTCCAGGAAAAACGACAGCGGTTTTTTGGTTTTTTTAGAC
GAATTTAAATCCTATGTGGAAAACGATTTGTTGAACACTAAAATCAACGCTTTAATCACGCAAGCCAGGAAAGCTAATGG
CGTGGTGGTGTTGGCTTTGCAAGACATTTACCAACTCAGCGGGGTCAAAAACGCCCATAGTTTTTTAAGCAACATGGGGA
CTCTTATTTTGTATCCGCAAAAAAACGCTAGGGAGTTGAAACACAATTTCAATGTGCCTTTGAGCGAGACTGAAATTTCT
TTTTTAGAAAACACCCCTTTGTATGCCAGGCAAGTTTTAGTCAAAAATCTGGGTAACGGGAGTTCTAACATGATTGATGT
GAGTTTGGAGGGCTTGGGGCGTTATTTGAAGATCTTTAATTCAGATTCTAGCCATGTGAATAAAGTGAAAGCGTTACAAA
AAGACTACCCTACAGAGTGGCGTGAGAAACTTTTGAAGAGTTAG

Domains

Predicted by InterproScan.

CagE, TrbE, VirB family, component of type IV transporter system (CagE_TrbE_VirB)

(176-375)

FtsK/SpoIIIE family (FtsK_SpoIIIE)

(426-466)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comB4 Helicobacter pylori 26695

97.205

100

0.972

Multiple sequence alignment