Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilS   Type   Regulator
Locus tag   GZH78_RS25550 Genome accession   NZ_CP048408
Coordinates   5693281..5694870 (+) Length   529 a.a.
NCBI ID   WP_163976903.1    Uniprot ID   -
Organism   Pseudomonas fluorescens strain DR397     
Function   regulate pilin expression (predicted from homology)   
Competence regulation

Genomic Context

Location: 5688281..5699870
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GZH78_RS25530 (GZH78_25540) pgeF 5690082..5690804 (-) 723 WP_103385452.1 peptidoglycan editing factor PgeF -
  GZH78_RS25535 (GZH78_25545) rluD 5690801..5691763 (-) 963 WP_011335991.1 23S rRNA pseudouridine(1911/1915/1917) synthase RluD -
  GZH78_RS25540 (GZH78_25550) - 5691916..5692932 (+) 1017 WP_007959566.1 outer membrane protein assembly factor BamD -
  GZH78_RS25545 (GZH78_25555) - 5693058..5693291 (+) 234 WP_039771683.1 PP0621 family protein -
  GZH78_RS25550 (GZH78_25560) pilS 5693281..5694870 (+) 1590 WP_163976903.1 ATP-binding protein Regulator
  GZH78_RS25555 (GZH78_25565) pilR 5694867..5696213 (+) 1347 WP_163976505.1 sigma-54 dependent transcriptional regulator Regulator
  GZH78_RS25560 (GZH78_25570) thiO 5696273..5697373 (-) 1101 WP_163976506.1 glycine oxidase ThiO -
  GZH78_RS25565 (GZH78_25575) - 5697431..5697832 (-) 402 WP_085668039.1 type IV pilin protein -

Sequence

Protein


Download         Length: 529 a.a.        Molecular weight: 58636.37 Da        Isoelectric Point: 6.5586

>NTDB_id=421123 GZH78_RS25550 WP_163976903.1 5693281..5694870(+) (pilS) [Pseudomonas fluorescens strain DR397]
MIAEADNADSKQAQRLLRLYHLYRLSVGITLVLLISSNMDNRLLTSANDEWLRNGSWLYLVLNILLVVFLENIRRPAQLF
ALALVDILLLCGVFYAAGGVASAIGNLLIVSVAISNTLLRRRIGLLIAAIATLGIVGLSFLLSFSHPLSANDYLQAGTLG
ALCFAASLLVQGLMRRLEVSENLAELRASEVVGLEALNALILQRMRTGILVLDEQRRVQLANHSARTLLGQTHLEGDLID
EHSSALVERLQLWLNNPTLRPQSLKIAGNGLELQPSFIALEQSPNRQTLVFLEDLAQIAQQAQQLKLAALGRLTAGIAHE
IRNPLGAISHAAQLLQESEELDGADRRLTQIIQDHSQRMNRVIENVLQLSRRQQSAPQRLDLKPWLEQFVDESREQAGER
QHIHLHIGPGDFRTLMDPGQLTQILDNLLRNGWRHSALLHDPAEVWLTLSIDPESTLAVLEVQDNGPGVPLDQQAHLFEP
FFTTSSQGTGLGLYLSRELCESNQARLDFKSRQGGGCFRITFAHGRKQS

Nucleotide


Download         Length: 1590 bp        

>NTDB_id=421123 GZH78_RS25550 WP_163976903.1 5693281..5694870(+) (pilS) [Pseudomonas fluorescens strain DR397]
GTGATCGCTGAGGCTGACAACGCCGACAGCAAACAGGCCCAGCGACTGCTGCGTCTCTATCATCTGTACCGTTTAAGTGT
CGGCATCACCCTGGTGCTGCTGATCTCCAGCAACATGGACAACCGCCTGCTGACCTCGGCCAACGACGAGTGGCTGCGCA
ATGGCAGCTGGTTGTACCTGGTGCTGAACATCCTGCTGGTGGTGTTCCTCGAGAACATCCGCCGCCCGGCCCAGTTGTTC
GCTCTGGCACTGGTCGACATTCTGCTGCTGTGCGGCGTGTTCTACGCAGCGGGCGGTGTGGCCAGTGCGATTGGCAACTT
GCTGATCGTTTCGGTGGCCATCAGCAACACCTTGCTGCGACGACGTATCGGCTTGCTGATCGCCGCCATCGCCACCCTCG
GCATCGTCGGGCTCAGCTTTCTGCTGAGTTTCAGCCATCCCCTGAGCGCCAACGACTATCTGCAGGCCGGCACCCTCGGC
GCCTTGTGCTTCGCCGCCTCGCTGCTGGTGCAGGGATTGATGCGCCGTCTCGAAGTCAGTGAGAACCTGGCCGAGCTGCG
CGCCAGCGAAGTGGTGGGGCTCGAAGCCCTCAACGCGCTGATCCTGCAACGCATGCGCACCGGCATTCTGGTGCTCGACG
AACAGCGCCGGGTGCAACTGGCCAACCACAGCGCGAGGACCCTCCTGGGCCAAACACACCTTGAAGGCGACTTGATCGAT
GAGCACTCGAGCGCATTGGTCGAACGCCTGCAACTGTGGCTGAACAACCCGACCCTGCGCCCGCAAAGCCTGAAAATCGC
CGGCAATGGCCTGGAGCTGCAGCCGAGTTTCATCGCCCTGGAACAGAGTCCGAACCGCCAGACCCTGGTGTTTCTCGAAG
ACCTCGCCCAGATCGCCCAGCAGGCGCAGCAATTGAAACTGGCCGCGCTGGGCCGGCTGACCGCAGGTATCGCCCATGAG
ATCCGCAATCCGCTGGGGGCCATCAGTCATGCCGCGCAGCTGTTGCAGGAATCCGAGGAACTCGACGGCGCGGATCGGCG
TCTGACGCAGATCATTCAAGACCACTCCCAGCGCATGAACCGAGTCATCGAAAACGTCCTGCAACTGTCCCGCCGCCAGC
AGAGCGCACCGCAACGGCTCGACCTCAAACCGTGGCTGGAGCAGTTCGTCGACGAGAGCCGCGAACAGGCCGGCGAGCGC
CAGCACATTCATCTGCACATCGGCCCGGGGGATTTCCGCACGTTGATGGATCCCGGCCAGCTCACGCAGATTCTCGACAA
TCTGTTACGCAACGGCTGGCGCCACAGCGCCCTGTTGCACGATCCGGCCGAGGTCTGGCTGACGCTATCCATCGACCCCG
AGAGCACGCTGGCCGTGCTCGAAGTGCAAGACAACGGCCCCGGCGTGCCGCTCGATCAACAGGCTCACCTGTTCGAACCG
TTCTTTACCACCAGCAGCCAGGGCACCGGCCTTGGGCTTTATCTGTCCCGTGAGCTGTGCGAAAGCAATCAAGCGCGCCT
AGACTTCAAATCACGCCAAGGCGGCGGCTGCTTTCGCATCACCTTTGCTCACGGACGGAAACAAAGTTGA

Domains

Predicted by InterproScan.

Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (HATPase_c)

(417-521)

His Kinase A (phospho-acceptor) domain (HisKA)

(309-374)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilS Pseudomonas aeruginosa PAK

60.566

100

0.607

Multiple sequence alignment