Detailed information NTG0420782

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: GZS10_RS22160
• Genome accession: NZ_CP048349
• Coordinates: 4676682..4677479 (+)
• Length: 265 a.a.
• NCBI ID: WP_004858028.1
• Uniprot ID: -
• Organism: Raoultella ornithinolytica strain 23
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4671682..4682479

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GZS10_RS22125 (GZS10_22125)	gspH	4671835..4672320 (+)	486	WP_233471560.1	type II secretion system minor pseudopilin GspH	-
GZS10_RS22130 (GZS10_22130)	gspI	4672317..4672682 (+)	366	WP_004858044.1	type II secretion system minor pseudopilin GspI	-
GZS10_RS22135 (GZS10_22135)	gspJ	4672694..4673278 (+)	585	WP_015585121.1	type II secretion system minor pseudopilin GspJ	-
GZS10_RS22140 (GZS10_22140)	gspK	4673275..4674261 (+)	987	WP_044348325.1	type II secretion system minor pseudopilin GspK	-
GZS10_RS22145 (GZS10_22145)	gspL	4674248..4675450 (+)	1203	WP_004858036.1	type II secretion system protein GspL	-
GZS10_RS22150 (GZS10_22150)	gspM	4675443..4675937 (+)	495	WP_032716581.1	type II secretion system protein GspM	-
GZS10_RS22155 (GZS10_22155)	-	4675927..4676667 (+)	741	WP_097357756.1	type II secretion system protein N	-
GZS10_RS22160 (GZS10_22160)	pilD	4676682..4677479 (+)	798	WP_004858028.1	prepilin peptidase	Machinery gene
GZS10_RS22165 (GZS10_22165)	hrpB	4677500..4679929 (-)	2430	WP_162661651.1	ATP-dependent helicase HrpB	-
GZS10_RS22170 (GZS10_22170)	thpR	4680002..4680538 (+)	537	WP_004858022.1	RNA 2',3'-cyclic phosphodiesterase	-
GZS10_RS22175 (GZS10_22175)	sfsA	4680538..4681254 (+)	717	WP_004858020.1	DNA/RNA nuclease SfsA	-
GZS10_RS22180 (GZS10_22180)	dksA	4681419..4681874 (+)	456	WP_004858018.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 265 a.a.
• Molecular weight: 29315.95 Da
• Isoelectric Point: 8.4762

>NTDB_id=420782 GZS10_RS22160 WP_004858028.1 4676682..4677479(+) (pilD) [Raoultella ornithinolytica strain 23]
MTLLSAFSLQFPLLWGGLLFIFGLTFGSFFNVVIYRLPLMMQQEESARFNLCVPASFCPQCQRPIIWRDNIPLLSYLSLK
GRARCCQAPISQRYPLTELASGLLFVLTGYLLTPGLPLLGGLILLSTLLVLAIIDGQTQLLPDRLTLPLLWAGLLFNLNG
TFVPLSEAVIGAMTGYLSLWTVYWLFRLLTGKEALGYGDFKLLAALGAWSGWQILPQTLLCASASGLIWTLLQRRITLQS
LDQPLAFGPWLALAGSGLFLWTQTL

Nucleotide

• Length: 798 bp

>NTDB_id=420782 GZS10_RS22160 WP_004858028.1 4676682..4677479(+) (pilD) [Raoultella ornithinolytica strain 23]
ATGACGCTACTGAGCGCATTTTCGTTGCAGTTCCCCCTGTTATGGGGCGGATTGTTGTTTATTTTCGGCCTGACGTTTGG
CAGCTTTTTTAACGTGGTTATCTACCGTTTGCCGCTGATGATGCAGCAGGAAGAGTCCGCACGATTCAACCTGTGCGTTC
CCGCCTCCTTCTGCCCCCAGTGCCAGCGGCCCATCATCTGGCGGGATAATATTCCGCTGCTCAGCTATCTCTCACTCAAA
GGGCGCGCCCGCTGCTGCCAGGCGCCCATTTCTCAGCGCTATCCGCTGACGGAGCTGGCCAGCGGGCTGCTCTTTGTGTT
GACCGGCTATCTGCTGACGCCGGGATTACCGCTGCTGGGGGGCCTGATCCTGCTCTCCACGCTGCTGGTACTGGCGATCA
TCGACGGCCAGACTCAGCTCCTGCCGGATCGGCTGACTCTGCCGCTGCTGTGGGCCGGTCTGCTGTTTAATCTCAACGGC
ACGTTTGTCCCGCTCAGCGAAGCGGTGATCGGCGCCATGACGGGCTATTTATCACTGTGGACGGTTTACTGGCTGTTTCG
CCTGCTGACCGGCAAAGAGGCGCTAGGATACGGCGATTTTAAGCTGCTGGCGGCACTCGGCGCATGGTCCGGCTGGCAGA
TCCTGCCGCAGACGCTGCTGTGCGCCTCGGCCAGCGGCCTGATCTGGACCTTGCTGCAACGGCGGATCACGCTCCAGTCG
CTGGATCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCGGGCAGCGGCCTGTTTCTGTGGACTCAGACGCTGTAA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (122-230)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	48.529	100	0.498
pilD	Vibrio campbellii strain DS40M4	45.221	100	0.464
pilD	Neisseria gonorrhoeae MS11	46.614	94.717	0.442
pilD	Acinetobacter baumannii D1279779	44.574	97.358	0.434
pilD	Acinetobacter nosocomialis M2	44.186	97.358	0.43