Detailed information NTG0420438

Bioinformatically predicted

Overview

• Name: addA
• Type: Machinery gene
• Locus tag: M654_RS07640
• Genome accession: NZ_CP048273
• Coordinates: 1484773..1488465 (+)
• Length: 1230 a.a.
• NCBI ID: WP_077737082.1
• Uniprot ID: A0A6I7F6A0
• Organism: Bacillus sp. NSP9.1
• Function: homologous recombination; plasmid transformation (predicted from homology) Homologous recombination

Genomic Context

Location: 1479773..1493465

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
M654_RS07630 (M654_007615)	modB	1480519..1481175 (+)	657	WP_026586327.1	molybdate ABC transporter permease subunit	-
M654_RS07635 (M654_007620)	addB	1481286..1484813 (+)	3528	WP_026586328.1	helicase-exonuclease AddAB subunit AddB	Machinery gene
M654_RS07640 (M654_007625)	addA	1484773..1488465 (+)	3693	WP_077737082.1	helicase-exonuclease AddAB subunit AddA	Machinery gene
M654_RS07645 (M654_007630)	sbcD	1488523..1489704 (+)	1182	WP_026586329.1	exonuclease subunit SbcD	-
M654_RS07650 (M654_007635)	sbcC	1489701..1493099 (+)	3399	WP_026586330.1	exonuclease subunit SbcC	-
M654_RS07655 (M654_007640)	-	1493167..1493385 (-)	219	WP_026586331.1	spore germination protein	-

Sequence

Protein

• Length: 1230 a.a.
• Molecular weight: 140738.45 Da
• Isoelectric Point: 5.5682

>NTDB_id=420438 M654_RS07640 WP_077737082.1 1484773..1488465(+) (addA) [Bacillus sp. NSP9.1]
MDMTKPKGSTWTDDQWKAIVSSGRDILVAAAAGSGKTAVLVERIIRKITKKENPIDVDRLLVVTFTNASAAEMKHRIGEA
LEKEIAENPGSLHLRRQLALLNRASISTLHSFCLQVIRKYYYLIDIDPAFRIADQTEGELLGDEVLDELFEDEYKKGDPA
FFELVDRYTTDRHDLDLQYLVKRVYEFSRSHPDPEGWLITFADLYDAGQDTGVESLPFYPYIKEDIALVLEGAREKLARA
LELAKQPGGPAPRAENFADDLAQIDRLIEHKDDFSALYELVPAVSFKRAKPCKGDEFEPALIDEATELRNGAKKQLEKLK
SDYFARTPDQHLTDLKAMKPVIDTLVKLVLEYGKRYKAAKQEKAIVDFSDLEHDCLAILAQKNEAGETVPSEAAKHYRHQ
FHEVLVDEYQDTNLVQEAILKLVAKEEHEGNLFMVGDVKQSIYRFRLAEPLLFLSKYKRFTEDGSGPGQKIDLNKNFRSR
SDILDSTNFLFKQLMGEKVGEVEYDEQAELKLGASYPPNEETKTELVIIEQQEQAADEEAEELETVQLEARAMAGQIRKL
IAEKFQVYDGKTKTTRNIQYRDIVVLLRSMPWAPQIMDEFKQQGIPVYANLSTGYFEAAEVSVTLSLLKVIDNAYQDIPL
ASVLRSPVVGLDENELSLIRIQDKKATFYEAMKAYLALADRNDSLYDKLRRFEGLLKKWRVFAKNHSVAELIWEIYRDTK
YLDYVGGMPGGKQRQANLRALYDRARSYEATSFRGLFRFLRFIERMQERGDDLGTARALSEQEDVVRLMTIHSSKGLEFP
VVFTAGIGRSFNMMDLNKPYLLDKELGFGTKFIHPKWRISYPTLPLVAMKKKMRRELLSEELRVLYVALTRAKEKLYLLG
SAKDKEKLLAKWRTSAARPGWLLPDFDRFQAKSYLDFVGPAVVRHRDMAGVGLDSADDEIRHHPSRFQISWLSAADLQAE
NGKQAGDEKQERLAQIQMGQPINARFDYQKEVGERLTWSYPHQDAAKIRTKQSVSEIKRQKEYEDEYGDRSLIRQTNESH
LFRRPSFMMAKGLTAAERGTAMHTVMQHIPLSKAPEQDELSLFLDRLVQHELLTEEQRAAIDEDDILAFFETEIGEKLLG
ARRVEREVPFNMTLPAGEVYPELQTADEPVLIQGIIDCLFETADGFYLLDYKTDRIQGKYANGMADAEPALRKRYETQIQ
LYSRAVETMLKTPLAGRALYFFDGGHVLKF

Nucleotide

• Length: 3693 bp

>NTDB_id=420438 M654_RS07640 WP_077737082.1 1484773..1488465(+) (addA) [Bacillus sp. NSP9.1]
GTGGACATGACGAAGCCAAAGGGCAGCACATGGACAGATGATCAATGGAAGGCGATTGTCTCATCCGGCCGGGACATATT
AGTTGCCGCAGCGGCGGGGTCCGGAAAAACGGCCGTTCTTGTCGAACGGATCATCCGAAAAATTACGAAAAAGGAAAACC
CGATTGATGTGGACAGGCTGCTTGTCGTGACCTTTACGAATGCATCGGCGGCAGAGATGAAGCATCGGATCGGTGAAGCG
CTTGAAAAAGAGATTGCCGAAAACCCCGGCTCCCTTCACTTGCGCAGACAGCTCGCTTTATTGAACAGGGCCAGCATCTC
AACACTGCATTCATTCTGCCTGCAGGTCATTCGCAAATATTATTATCTGATCGACATCGATCCGGCTTTTCGGATCGCCG
ATCAGACCGAAGGCGAACTGCTTGGCGATGAGGTGCTTGATGAGCTGTTTGAAGATGAATATAAAAAAGGAGATCCCGCC
TTTTTCGAACTTGTGGATCGATATACGACAGACCGCCATGATCTTGATTTGCAGTATCTTGTGAAGCGCGTCTATGAATT
TTCACGGTCACATCCCGATCCGGAAGGCTGGCTTATAACATTTGCCGACCTTTATGACGCCGGTCAAGACACGGGTGTGG
AGTCACTGCCGTTCTATCCTTATATTAAGGAAGATATCGCGCTCGTACTTGAAGGGGCGCGGGAAAAGCTTGCGCGTGCG
CTGGAGCTGGCCAAACAGCCGGGCGGACCCGCTCCCCGCGCCGAAAACTTCGCGGACGATTTGGCGCAGATCGATAGGCT
GATCGAGCACAAGGATGACTTTTCCGCCCTTTATGAGCTTGTTCCCGCTGTATCGTTCAAAAGGGCAAAACCGTGCAAAG
GAGACGAGTTTGAGCCCGCATTAATCGATGAAGCGACAGAGCTCCGCAACGGTGCGAAAAAACAGCTGGAAAAACTGAAA
AGCGATTATTTTGCCCGGACCCCTGATCAGCATTTGACTGACCTGAAGGCGATGAAGCCTGTCATTGATACGCTTGTCAA
GCTTGTTCTGGAATATGGAAAGCGCTATAAGGCGGCGAAGCAAGAAAAGGCGATCGTGGATTTCTCTGATCTTGAGCACG
ATTGCCTGGCGATTTTGGCGCAAAAAAACGAAGCCGGCGAAACAGTGCCGAGTGAAGCGGCGAAGCATTACCGGCACCAG
TTCCATGAGGTGCTTGTCGATGAGTACCAAGACACCAACCTTGTGCAGGAAGCGATTTTAAAGCTGGTCGCCAAAGAGGA
GCATGAGGGGAACCTGTTTATGGTGGGCGATGTCAAACAGTCGATTTACCGCTTTCGCCTCGCAGAACCGCTTTTGTTTC
TCTCGAAATATAAACGGTTTACAGAGGACGGCTCAGGCCCGGGCCAAAAAATCGATTTGAACAAAAACTTCCGCAGCCGT
TCAGACATATTGGACAGCACCAATTTCTTGTTTAAGCAGCTGATGGGGGAAAAAGTCGGTGAAGTGGAATATGACGAACA
AGCCGAATTGAAGCTCGGCGCATCTTACCCTCCGAATGAAGAAACAAAAACAGAGCTTGTGATCATCGAACAACAGGAAC
AAGCCGCCGATGAAGAAGCGGAGGAGCTTGAAACGGTTCAGCTGGAAGCGCGGGCCATGGCCGGACAGATCCGCAAACTG
ATTGCGGAGAAATTTCAGGTCTATGACGGCAAAACGAAAACGACCCGCAATATCCAATACCGCGATATTGTTGTTTTGCT
GCGGTCCATGCCATGGGCGCCGCAGATTATGGACGAGTTCAAACAGCAGGGGATTCCCGTTTATGCGAATTTGTCGACAG
GCTATTTTGAAGCGGCTGAAGTTTCCGTCACGCTTTCATTATTAAAAGTCATTGACAATGCGTACCAGGACATCCCGCTC
GCCTCTGTGCTCAGATCTCCTGTCGTCGGCCTTGATGAAAATGAGCTTTCCCTGATCAGGATTCAGGACAAGAAAGCGAC
GTTCTACGAAGCGATGAAGGCTTACCTCGCGCTTGCCGACCGCAATGACAGCCTTTACGATAAGCTCCGCCGCTTTGAAG
GGCTTCTGAAAAAATGGAGAGTGTTTGCGAAAAACCACTCGGTAGCTGAGCTGATTTGGGAAATTTACCGCGATACGAAA
TACTTGGATTATGTTGGCGGAATGCCGGGCGGAAAACAGCGGCAGGCCAACCTGCGCGCGCTTTACGACCGGGCGAGATC
ATATGAAGCGACTTCATTCCGCGGCCTGTTCCGCTTTTTAAGATTTATTGAGCGGATGCAGGAGCGCGGCGATGACCTCG
GAACGGCAAGAGCTCTCAGCGAACAGGAGGATGTCGTGCGGCTGATGACGATCCATTCAAGCAAAGGGCTGGAATTTCCT
GTCGTGTTCACGGCAGGGATCGGCCGGAGCTTTAATATGATGGATCTGAACAAACCGTATCTGCTCGATAAAGAGCTCGG
CTTCGGCACGAAATTCATCCATCCAAAATGGCGGATCAGCTATCCGACGCTGCCTCTTGTGGCGATGAAGAAAAAAATGC
GCCGCGAACTTTTGTCAGAGGAACTGCGCGTCTTGTACGTCGCACTGACACGTGCGAAGGAAAAGCTTTATTTGCTCGGA
TCGGCAAAAGATAAGGAAAAGCTCTTGGCGAAATGGCGAACTTCGGCTGCGCGGCCGGGATGGCTGCTGCCGGACTTTGA
CCGCTTTCAGGCCAAATCTTACTTGGATTTTGTCGGTCCGGCCGTAGTGCGTCACAGGGATATGGCAGGTGTTGGCCTCG
ATTCGGCGGATGACGAAATCCGGCATCACCCGTCCCGCTTTCAGATCAGCTGGCTCTCCGCCGCTGACCTTCAAGCCGAA
AACGGTAAACAAGCCGGGGACGAAAAACAGGAAAGGCTGGCCCAAATTCAAATGGGACAGCCGATCAATGCCCGGTTTGA
CTATCAAAAAGAAGTCGGAGAACGGCTGACATGGTCATATCCACATCAAGACGCAGCGAAAATCCGCACCAAACAGTCTG
TTTCCGAAATCAAGAGGCAAAAGGAATATGAGGACGAGTACGGTGACCGTTCATTGATCCGCCAGACAAATGAGTCACAC
CTGTTCAGACGGCCATCCTTTATGATGGCGAAGGGACTAACCGCGGCCGAACGCGGAACGGCGATGCACACCGTCATGCA
GCACATCCCGCTTTCGAAAGCTCCGGAACAAGACGAGCTCAGCCTCTTTCTCGACAGGCTTGTTCAACATGAGCTTTTAA
CAGAAGAGCAGCGCGCTGCAATCGATGAAGACGATATTCTCGCGTTTTTCGAAACGGAGATCGGTGAAAAGCTCCTCGGC
GCCCGCCGGGTGGAGCGTGAAGTTCCCTTTAATATGACGCTTCCGGCCGGAGAGGTTTATCCCGAATTGCAGACAGCGGA
CGAACCCGTGTTGATTCAGGGAATAATCGACTGTTTGTTTGAGACGGCTGACGGGTTTTATTTGCTCGATTACAAAACAG
ACCGGATACAAGGGAAATACGCAAACGGCATGGCCGACGCCGAGCCGGCTTTAAGGAAACGCTATGAAACTCAGATTCAA
CTTTATTCACGCGCTGTTGAAACAATGCTCAAGACCCCGCTTGCAGGCCGCGCCCTGTACTTTTTTGACGGCGGGCATGT
ACTCAAGTTTTAA

Domains

Predicted by InterproScan.

• UvrD-like helicase C-terminal domain (UvrD_C) (471-879)

• UvrD/REP helicase N-terminal domain (UvrD-helicase) (11-460)

• PD-(D/E)XK nuclease superfamily (PDDEXK_1) (1050-1222)

3D structure

• AlphaFold DB: A0A6I7F6A0

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
addA	Bacillus subtilis subsp. subtilis str. 168	72.602	100	0.726