Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   M654_RS07635 Genome accession   NZ_CP048273
Coordinates   1481286..1484813 (+) Length   1175 a.a.
NCBI ID   WP_026586328.1    Uniprot ID   A0A6I7FBW4
Organism   Bacillus sp. NSP9.1     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1476286..1489813
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M654_RS07610 (M654_007595) - 1476922..1478289 (-) 1368 WP_026586323.1 HAMP domain-containing sensor histidine kinase -
  M654_RS07615 (M654_007600) - 1478270..1478962 (-) 693 WP_026586324.1 response regulator transcription factor -
  M654_RS07620 (M654_007605) - 1479084..1479527 (-) 444 WP_026586325.1 ferritin-like domain-containing protein -
  M654_RS07625 (M654_007610) modA 1479716..1480510 (+) 795 WP_026586326.1 molybdate ABC transporter substrate-binding protein -
  M654_RS07630 (M654_007615) modB 1480519..1481175 (+) 657 WP_026586327.1 molybdate ABC transporter permease subunit -
  M654_RS07635 (M654_007620) addB 1481286..1484813 (+) 3528 WP_026586328.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  M654_RS07640 (M654_007625) addA 1484773..1488465 (+) 3693 WP_077737082.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  M654_RS07645 (M654_007630) sbcD 1488523..1489704 (+) 1182 WP_026586329.1 exonuclease subunit SbcD -

Sequence

Protein


Download         Length: 1175 a.a.        Molecular weight: 135177.15 Da        Isoelectric Point: 5.4936

>NTDB_id=420437 M654_RS07635 WP_026586328.1 1481286..1484813(+) (addB) [Bacillus sp. NSP9.1]
MGAVFLSGRSGSGKTTFILNEIREKLRHEPLGKPIIFLVPDQMTFLMEYELSKTHDLGGTIRAQVYSFSRLAWRILQHTG
GANRPFLTGTGIQMLLRKLIEEHKEEFKVYQNASDKSGFTEQVERMLTEFKRHCLTPETIRGMMQGSGVSEYEDERILAD
KLHDLYILYSKLEEHLENRYVQSEDYLTLLAEQIPYAEEIKNASVYVDGFHQFTPQEMEVLEQLMAHAGEMTFSLTADKP
FTNNIPHELHLFRMTGKAYYDLYQKAQALGLDLTEVRLEDDKRHKDAPELRHLERHFDERPIKAYTGKMEALSIMQAAGR
RTEIEGIAREIHSLVRKGTHRLRDIAVIARNVEDYKDTIKEVFTDYDIPFFIDGKESMQNHPLIELIRSTLDIIKGNWRY
EAVFRCIKTELLFPQDQPAERLREQIDQLENYCIAYGIKGERWTSKDPFTYRRYASLDEDFAKTDQEIETERMLNELKGW
IVPPIHTLQKRLKKAGTVREMAEALYLYLEEADVPMKLEQERRMAEEDGRIAESRQHDQVWDAVIQLLDEFVEMMGEEHI
SFSLFQQMIDTGLESLKFALIPPALDQVFIGNMDLSRMYKTSCAFLIGANDGVLPARPADDGVLSDDDREWLKRNGAELA
ATGREQLLDETFLIYMALSSPSEKLYVSYPIADPEGKTLLPSTVVKQLHELFPDHGEKMLVNEPEQVSDEAQLEFLVNKS
AALSYLAGQLRLWTRQYSISDVWWSTYNFLMNEPDRLFSRNILSSLFFRNQVQKLNQQVSRDLYGENIQGSVSRMETYKA
CPFSHFASHGLKLKERQFFKLEAPDIGQLFHSALKLISDRLHELKLEWRDLTKEQCDTLSTDAVERLAPKLQKEILLSSN
RHHYVKQKLQKIISRVSGILSEHAKASGFAPVGLELGFGGNGPLPPMRFTLKNGCTMELVGRIDRVDKAESSKGLLLRIV
DYKSSDKGLDLAEVYYGLALQMLTYLDLSITHSADWLGMKATPAGVLYFHVHDPMIQASVPLGLDEIEKEIFKQFKMKGL
LLGDQEAIRLMDTTLEQGRSDIISAGLKKDGSLRSDSAVVTEDDFHLLRRHIRRTFQEAGEEITDGNVSIEPYKLKDKTP
CTYCAFKSFCQFDESLEENEYRMLKQEKDNVILERLKKEGETSGHDEAKGQHMDR

Nucleotide


Download         Length: 3528 bp        

>NTDB_id=420437 M654_RS07635 WP_026586328.1 1481286..1484813(+) (addB) [Bacillus sp. NSP9.1]
GTGGGAGCAGTATTTTTATCAGGCAGGTCCGGGAGTGGAAAAACGACCTTTATTTTAAATGAAATCAGAGAAAAACTCCG
CCATGAACCGCTCGGAAAACCGATCATTTTTCTAGTCCCGGATCAAATGACGTTTTTAATGGAATACGAGCTTAGCAAAA
CACATGATTTGGGCGGCACCATCCGCGCCCAGGTCTACAGTTTTTCAAGGCTTGCCTGGCGAATTCTCCAGCACACCGGA
GGCGCAAACCGCCCGTTTTTGACGGGAACAGGGATTCAAATGCTGCTTCGCAAACTGATCGAAGAGCATAAGGAGGAATT
CAAGGTCTATCAAAATGCGAGCGATAAGAGCGGATTTACGGAGCAGGTGGAACGGATGCTGACCGAATTCAAACGGCATT
GCCTCACACCTGAAACCATCCGCGGGATGATGCAGGGAAGCGGGGTGTCGGAATATGAGGATGAACGGATTCTTGCCGAT
AAGCTGCACGATTTATATATTTTGTACAGCAAACTGGAAGAACATCTCGAGAACCGGTACGTTCAGTCGGAAGATTACTT
GACGCTCCTGGCCGAGCAGATCCCGTATGCCGAAGAGATCAAAAACGCTTCCGTCTATGTGGACGGCTTTCATCAATTCA
CACCGCAGGAGATGGAGGTGCTTGAACAGCTGATGGCGCATGCCGGGGAGATGACGTTTTCTTTGACAGCGGACAAGCCG
TTTACAAACAACATTCCCCATGAACTGCATCTATTTCGGATGACGGGCAAGGCGTATTATGATCTCTATCAAAAAGCGCA
GGCGTTAGGCCTTGACCTGACAGAAGTTCGTCTTGAAGATGATAAGCGGCATAAGGACGCTCCGGAACTCCGGCATTTGG
AACGGCATTTTGATGAACGGCCGATAAAAGCGTATACAGGAAAAATGGAGGCGCTCTCGATCATGCAGGCGGCCGGCAGA
AGAACCGAAATCGAGGGGATCGCGCGCGAAATTCATTCCCTTGTCCGCAAAGGAACGCATCGACTGAGAGATATCGCCGT
CATCGCCCGCAATGTCGAAGACTATAAGGATACGATAAAAGAAGTCTTTACCGATTACGATATTCCTTTTTTCATAGACG
GCAAGGAATCGATGCAGAATCATCCGCTGATCGAATTGATCCGTTCAACCCTTGATATCATCAAGGGCAACTGGAGATAT
GAAGCGGTTTTCCGCTGCATCAAAACAGAGCTGTTATTTCCGCAGGATCAGCCGGCTGAACGGCTCAGGGAGCAGATTGA
CCAGCTCGAGAACTACTGCATCGCCTACGGCATAAAAGGAGAAAGGTGGACAAGCAAGGATCCGTTCACCTATCGAAGAT
ATGCCTCCTTGGACGAGGACTTTGCCAAGACAGATCAGGAAATAGAGACTGAGCGGATGCTCAACGAATTGAAAGGCTGG
ATTGTCCCGCCTATTCACACGCTGCAAAAGCGCTTGAAAAAAGCCGGAACAGTCAGGGAGATGGCAGAAGCGCTATACCT
TTACCTTGAAGAAGCCGATGTTCCGATGAAGCTTGAACAGGAAAGGCGGATGGCCGAAGAAGACGGCCGGATCGCGGAAT
CGCGGCAGCATGACCAGGTATGGGACGCCGTTATCCAGTTATTGGACGAGTTCGTCGAGATGATGGGAGAGGAACATATT
TCCTTTTCCCTGTTTCAGCAAATGATCGATACCGGGTTGGAGTCTTTGAAATTCGCGCTGATTCCGCCAGCACTCGATCA
GGTGTTTATCGGCAATATGGACCTTTCCAGAATGTATAAGACGAGCTGCGCATTTCTCATCGGCGCAAACGATGGCGTAT
TGCCGGCCCGGCCCGCTGATGACGGGGTTTTGTCTGATGACGACAGAGAATGGCTGAAACGAAACGGAGCCGAGCTTGCG
GCGACGGGCAGGGAACAGCTTCTGGATGAGACCTTTCTGATCTATATGGCGCTTTCAAGCCCGTCTGAAAAGCTTTACGT
TTCATATCCGATCGCCGATCCCGAAGGAAAGACACTCCTGCCTTCAACGGTTGTGAAGCAGCTGCACGAGCTGTTTCCCG
ATCACGGAGAAAAGATGCTGGTCAATGAGCCGGAACAAGTAAGCGATGAAGCGCAGCTTGAATTTCTTGTCAATAAAAGC
GCGGCTTTAAGCTATTTGGCAGGACAGCTCAGGCTTTGGACAAGACAATATTCGATCAGCGACGTATGGTGGAGTACATA
CAACTTTTTGATGAATGAGCCTGACCGGCTCTTTTCCCGAAACATTTTGTCGAGCTTGTTTTTCCGAAATCAAGTGCAAA
AGCTGAATCAGCAAGTGAGCCGCGATTTGTACGGGGAGAACATTCAGGGGAGCGTTTCACGGATGGAAACGTACAAAGCG
TGTCCGTTTTCACACTTTGCTTCACACGGCTTAAAGCTGAAGGAACGGCAGTTTTTCAAACTTGAAGCGCCCGACATCGG
CCAGCTGTTCCACTCGGCGTTGAAGCTGATCTCAGACCGGCTTCATGAGCTGAAACTGGAGTGGCGCGATCTGACGAAGG
AACAATGTGACACATTGTCCACCGATGCCGTGGAACGGCTCGCCCCAAAATTGCAAAAAGAGATTCTGCTGAGCTCAAAC
CGCCATCATTATGTGAAGCAGAAGCTGCAAAAAATCATTTCCAGGGTTTCCGGCATTTTAAGCGAACATGCGAAAGCGAG
CGGCTTCGCTCCCGTCGGTCTCGAGCTTGGATTCGGCGGGAACGGCCCGCTTCCGCCGATGCGGTTTACCTTGAAAAACG
GATGCACAATGGAGCTTGTCGGCCGAATTGACAGGGTCGACAAAGCGGAAAGTTCAAAAGGTCTGCTGCTTCGGATCGTT
GATTACAAATCGAGTGATAAAGGGCTTGATTTAGCAGAAGTCTATTACGGTCTCGCCCTGCAAATGCTGACATATCTTGA
TTTGTCCATCACCCATTCGGCGGACTGGCTCGGGATGAAGGCGACACCTGCTGGAGTTCTTTATTTTCATGTACATGATC
CGATGATTCAGGCTTCCGTGCCGCTGGGCCTTGATGAGATTGAAAAGGAAATCTTCAAGCAATTTAAGATGAAAGGTCTG
CTTTTAGGCGACCAGGAAGCGATCCGCTTGATGGATACGACCCTTGAACAGGGCCGATCAGACATCATCAGCGCCGGCCT
GAAAAAAGACGGTTCGCTCCGTTCGGATTCCGCTGTCGTCACAGAGGATGACTTTCATTTATTGAGGCGCCACATTCGCC
GGACGTTTCAAGAAGCCGGCGAGGAGATCACAGACGGCAACGTGTCGATTGAACCGTATAAATTAAAAGACAAAACACCG
TGCACATACTGCGCTTTTAAATCGTTCTGCCAGTTTGACGAATCGCTTGAGGAGAATGAATACCGTATGCTGAAGCAGGA
AAAAGACAATGTGATATTGGAGCGGTTGAAAAAGGAGGGTGAGACGAGTGGACATGACGAAGCCAAAGGGCAGCACATGG
ACAGATGA

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(790-1131)

UvrD-like helicase C-terminal domain (UvrD_C)

(309-669)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6I7FBW4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

74.678

99.149

0.74

Multiple sequence alignment