Detailed information NTG0420248

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: GYN04_RS08560
• Genome accession: NZ_CP048254
• Coordinates: 1639980..1640840 (+)
• Length: 286 a.a.
• NCBI ID: WP_003689814.1
• Uniprot ID: A0AA44U9R8
• Organism: Neisseria gonorrhoeae strain CT530
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1634980..1645840

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GYN04_RS08545 (GYN04_08295)	dapD	1635490..1636311 (-)	822	WP_003689804.1	2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase	-
GYN04_RS08550 (GYN04_08300)	pgi	1636521..1638164 (-)	1644	WP_003689806.1	glucose-6-phosphate isomerase	-
GYN04_RS08555 (GYN04_08305)	pilG	1638674..1639906 (+)	1233	WP_003689811.1	type 4 pilus assembly protein PilG	Machinery gene
GYN04_RS08560 (GYN04_08310)	pilD	1639980..1640840 (+)	861	WP_003689814.1	prepilin peptidase	Machinery gene
GYN04_RS08565 (GYN04_08315)	coaE	1640837..1641469 (+)	633	WP_003689816.1	dephospho-CoA kinase	-
GYN04_RS08570 (GYN04_08320)	-	1641462..1641671 (+)	210	WP_003696096.1	DNA gyrase inhibitor YacG	-
GYN04_RS08575 (GYN04_08325)	pilF	1641757..1643433 (-)	1677	WP_250328376.1	type IV-A pilus assembly ATPase PilB	Machinery gene
GYN04_RS08585 (GYN04_08335)	-	1643771..1644217 (-)	447	WP_003689818.1	DUF441 domain-containing protein	-
GYN04_RS08590 (GYN04_08340)	-	1644221..1645195 (-)	975	WP_003689819.1	polyprenyl synthetase family protein	-
GYN04_RS08595 (GYN04_08350)	rplU	1645419..1645727 (+)	309	WP_003691838.1	50S ribosomal protein L21	-

Sequence

Protein

• Length: 286 a.a.
• Molecular weight: 31332.34 Da
• Isoelectric Point: 8.9561

>NTDB_id=420248 GYN04_RS08560 WP_003689814.1 1639980..1640840(+) (pilD) [Neisseria gonorrhoeae strain CT530]
MSDLSVLSPFAVPLAAVLGLLVGSFLNVVIYRVPVMMERGWTVFAKEHLNLPLTDDESRTFNLMKPDSCCPKCRVPIRAW
QNIPIVSYLLLRGKCASCQTKISIRYPLIELLTGVLFGLVAWQYGWSWITLGGLILTAFLISLTFIDADTQYLPDSMTLP
LIWLGLIFNLDGGFVPLQSAVLGAVAGYSSLWLLCAVYKLLTGKTGMGNGDFKLIAALGAWLGISALPVLIFVSSLIGLV
AAIVMRVAKGRHFAFGPALTVSGWIIFTANDSVWRAVNWWLTHPVR

Nucleotide

• Length: 861 bp

>NTDB_id=420248 GYN04_RS08560 WP_003689814.1 1639980..1640840(+) (pilD) [Neisseria gonorrhoeae strain CT530]
ATGTCTGATTTGTCTGTATTGTCGCCGTTTGCCGTGCCTTTGGCAGCAGTTTTGGGGCTGCTGGTCGGCAGCTTCCTGAA
TGTCGTCATTTACCGCGTACCCGTTATGATGGAACGCGGCTGGACGGTATTTGCCAAAGAACATTTAAACCTGCCGCTGA
CCGACGATGAAAGCCGTACCTTCAACCTGATGAAGCCGGATTCCTGCTGTCCCAAATGCCGTGTGCCGATACGCGCGTGG
CAGAACATCCCGATTGTCAGTTACCTGCTCCTGCGCGGCAAATGCGCTTCCTGCCAAACCAAAATCAGCATACGTTATCC
CTTAATCGAGCTGCTGACCGGCGTATTGTTCGGGCTGGTCGCCTGGCAATACGGCTGGTCTTGGATTACGCTGGGCGGTT
TGATACTGACCGCGTTTCTGATTTCCCTGACCTTTATCGATGCGGACACCCAATACCTGCCCGACTCGATGACATTACCC
TTGATCTGGCTGGGGCTGATATTTAATTTGGACGGCGGCTTCGTGCCTTTGCAGTCTGCCGTTTTAGGTGCGGTTGCCGG
CTATAGTTCATTATGGCTCTTATGTGCAGTGTATAAACTGCTCACAGGAAAAACCGGTATGGGCAACGGAGATTTCAAAC
TGATTGCCGCATTGGGCGCGTGGCTCGGCATATCCGCATTGCCCGTGCTGATTTTTGTTTCCTCTCTGATCGGTTTGGTC
GCGGCAATCGTTATGCGCGTCGCCAAGGGGCGGCATTTTGCCTTCGGCCCCGCACTGACAGTTTCGGGCTGGATAATTTT
TACGGCAAACGATTCCGTATGGCGGGCGGTCAACTGGTGGCTGACCCATCCGGTGAGATGA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (17-124)

• Type IV leader peptidase family (Peptidase_A24) (134-242)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Neisseria gonorrhoeae MS11	100	100	1
pilD	Vibrio cholerae strain A1552	44.792	100	0.451
pilD	Vibrio campbellii strain DS40M4	45.652	96.503	0.441
pilD	Acinetobacter baumannii D1279779	47.727	92.308	0.441
pilD	Acinetobacter nosocomialis M2	47.727	92.308	0.441