Detailed information NTG0420075

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: AXA69_RS05855
• Genome accession: NZ_CP048223
• Coordinates: 1226171..1227046 (+)
• Length: 291 a.a.
• NCBI ID: WP_005314162.1
• Uniprot ID: A2T195
• Organism: Aeromonas salmonicida subsp. salmonicida strain J223
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1221171..1232046

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AXA69_RS05835 (AXA69_005820)	nadC	1221408..1222265 (+)	858	WP_005314155.1	carboxylating nicotinate-nucleotide diphosphorylase	-
AXA69_RS05840 (AXA69_005825)	tapA	1222600..1223028 (+)	429	WP_005314158.1	type IVa pilus major pilin TapA	-
AXA69_RS05845 (AXA69_005830)	pilB	1223032..1224738 (+)	1707	WP_005314160.1	PilB family type IVa pilus assembly ATPase TapB	Machinery gene
AXA69_RS05850 (AXA69_005835)	-	1225119..1226107 (+)	989	Protein_1132	type II secretion system F family protein	-
AXA69_RS05855 (AXA69_005840)	pilD	1226171..1227046 (+)	876	WP_005314162.1	prepilin peptidase	Machinery gene
AXA69_RS05860 (AXA69_005845)	coaE	1227066..1227680 (+)	615	WP_005314164.1	dephospho-CoA kinase	-
AXA69_RS05865 (AXA69_005850)	zapD	1227718..1228440 (+)	723	WP_005314166.1	cell division protein ZapD	-
AXA69_RS05870 (AXA69_005855)	yacG	1228450..1228644 (+)	195	WP_005314167.1	DNA gyrase inhibitor YacG	-
AXA69_RS05875 (AXA69_005860)	mutT	1228721..1229128 (-)	408	WP_005314168.1	8-oxo-dGTP diphosphatase MutT	-
AXA69_RS05880 (AXA69_005865)	-	1229137..1230309 (-)	1173	WP_005314169.1	tetratricopeptide repeat protein	-

Sequence

Protein

• Length: 291 a.a.
• Molecular weight: 32500.78 Da
• Isoelectric Point: 6.9383

>NTDB_id=420075 AXA69_RS05855 WP_005314162.1 1226171..1227046(+) (pilD) [Aeromonas salmonicida subsp. salmonicida strain J223]
MTLLLELAHGLPWLYFSLVFLFSLMIGSFLNVVIHRLPIMLEREWQAEYRSYFSSDTPQPEDDERYNLMVPRSCCPRCNH
PITALENIPLLSWLWLKGRCRGCQAAISARYPLVELLTALLSVVVAMTLTPGWGTLAALLLTWVLVALTFIDLDKMLLPD
QLTLPLLWGGLLFNLLGGYVPLGDAVIGAMAGYLVLWSLYWAFKLLTGKEGMGYGDFKLLAALGAWLGWQALPIVLLLSS
LVGAIFGIGLILLRNHHQSKPIPFGPYLAIAGWIALLWGDSITRWYLSTIL

Nucleotide

• Length: 876 bp

>NTDB_id=420075 AXA69_RS05855 WP_005314162.1 1226171..1227046(+) (pilD) [Aeromonas salmonicida subsp. salmonicida strain J223]
ATGACACTTCTTCTCGAGCTGGCACACGGCTTGCCATGGCTCTACTTCTCGCTGGTTTTTCTCTTCTCCCTGATGATCGG
CAGCTTTCTCAACGTGGTGATCCACCGCCTGCCCATCATGCTGGAACGGGAGTGGCAGGCCGAATATCGCAGCTATTTCA
GTTCAGACACGCCGCAGCCAGAAGATGACGAGCGCTACAATCTGATGGTGCCGCGCTCCTGCTGCCCTCGCTGCAATCAC
CCCATAACGGCCTTGGAAAACATTCCCCTGCTGAGCTGGCTCTGGCTCAAAGGTCGCTGCCGGGGCTGCCAGGCCGCCAT
TTCGGCTCGCTATCCGCTGGTGGAGCTGCTGACCGCGCTGCTCTCTGTGGTGGTTGCCATGACGCTCACCCCTGGCTGGG
GTACGCTGGCTGCCCTCCTGCTGACCTGGGTGCTGGTGGCGCTCACCTTTATCGATCTGGACAAGATGCTGCTGCCGGAC
CAGCTCACCTTGCCGCTACTGTGGGGCGGGCTGCTGTTCAACCTGCTGGGCGGATACGTGCCTCTCGGCGATGCTGTCAT
CGGCGCCATGGCAGGCTATCTGGTGCTGTGGAGCCTCTACTGGGCCTTCAAGCTGCTTACCGGCAAGGAGGGCATGGGCT
ATGGGGACTTCAAACTGCTGGCAGCGCTGGGAGCCTGGCTTGGCTGGCAGGCGCTGCCCATAGTACTGCTGCTCTCTTCT
CTGGTCGGCGCCATCTTCGGCATTGGCCTCATTTTGCTGCGCAACCATCACCAGAGCAAACCCATCCCCTTTGGCCCCTA
TCTCGCCATCGCAGGCTGGATCGCACTGCTCTGGGGCGATAGCATCACCCGCTGGTATCTCTCCACTATCCTCTGA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (140-248)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-128)

3D structure

• AlphaFold DB: A2T195

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	60.764	98.969	0.601
pilD	Vibrio campbellii strain DS40M4	55.556	98.969	0.55
pilD	Acinetobacter baumannii D1279779	55.39	92.44	0.512
pilD	Acinetobacter nosocomialis M2	55.39	92.44	0.512
pilD	Neisseria gonorrhoeae MS11	50.746	92.096	0.467