Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   M662_RS00785 Genome accession   NZ_CP048117
Coordinates   146942..148321 (+) Length   459 a.a.
NCBI ID   WP_008635853.1    Uniprot ID   A0A7Z2Y8A0
Organism   Bacillus sp. SB49     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 141942..153321
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M662_RS00765 (M662_00765) - 142207..142665 (+) 459 WP_026577050.1 CtsR family transcriptional regulator -
  M662_RS00770 (M662_00770) - 142687..143235 (+) 549 WP_008635844.1 UvrB/UvrC motif-containing protein -
  M662_RS00775 (M662_00775) - 143236..144306 (+) 1071 WP_008635845.1 protein arginine kinase -
  M662_RS00780 (M662_00780) clpC 144325..146784 (+) 2460 WP_008635846.1 ATP-dependent protease ATP-binding subunit ClpC Regulator
  M662_RS00785 (M662_00785) radA 146942..148321 (+) 1380 WP_008635853.1 DNA repair protein RadA Machinery gene
  M662_RS00790 (M662_00790) disA 148322..149395 (+) 1074 WP_008635854.1 DNA integrity scanning diadenylate cyclase DisA -
  M662_RS00795 (M662_00795) - 149550..150653 (+) 1104 WP_008635855.1 PIN/TRAM domain-containing protein -
  M662_RS00800 (M662_00800) ispF 150813..151955 (+) 1143 WP_235601451.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -

Sequence

Protein


Download         Length: 459 a.a.        Molecular weight: 50220.96 Da        Isoelectric Point: 8.3345

>NTDB_id=419945 M662_RS00785 WP_008635853.1 146942..148321(+) (radA) [Bacillus sp. SB49]
MAKRKTKFVCQECGYETPKWMGKCPGCNQWNTLVEETSAPAGNSRHVFQTSSTSAMTKPERITQIKSQKEPRLPTSMPEF
NRVLGGGIVAGSLVLIGGDPGIGKSTLLLQVSAQIAKKDFPVLYISGEESSRQTKLRAERLGVMSDELYVLPETNLQDVL
NQIDQIEPKFVVIDSIQTIFKEDITSAPGSVAQVRECTSHLMRIAKTKGIPIFIVGHVTKEGSIAGPRLLEHMVDAVLYF
EGERHHTFRILRSVKNRFGSTHEMGIFEMKEKGLEEVLNPSEIFLEERSQGAAGSIVVASMEGTRPVLVEIQALISPTAY
GNPRRMATGLDTNRVPLLMAVLEKRAGLLLQNQDAYVKVAGGVKLDEPAIDLAVALSIASSFRNKVSNGDDVVVGEVGLT
GEIRRVSRIEQRVQEAAKLGFKRAIIPKKNMDGWTPPSQIEVIGVSTVQEAMKVTLGES

Nucleotide


Download         Length: 1380 bp        

>NTDB_id=419945 M662_RS00785 WP_008635853.1 146942..148321(+) (radA) [Bacillus sp. SB49]
TTGGCAAAAAGAAAAACAAAGTTCGTTTGCCAGGAATGTGGTTATGAAACACCGAAATGGATGGGGAAATGTCCGGGGTG
TAATCAGTGGAATACGTTGGTGGAAGAGACGTCTGCTCCGGCAGGAAACTCTCGGCACGTATTTCAGACAAGTTCCACTT
CCGCAATGACGAAGCCAGAAAGAATCACACAGATAAAATCACAGAAGGAACCACGCCTCCCGACAAGCATGCCGGAGTTC
AACAGAGTGCTCGGCGGCGGGATCGTGGCCGGATCATTAGTTTTAATCGGCGGGGATCCGGGTATCGGGAAGTCAACCTT
ACTGCTTCAAGTATCTGCTCAGATTGCAAAGAAAGATTTTCCGGTTTTATATATATCAGGAGAAGAATCCTCCAGGCAGA
CTAAACTCAGGGCCGAACGCCTTGGGGTCATGTCGGATGAACTGTATGTCCTGCCCGAGACGAACCTGCAAGATGTACTG
AATCAAATAGATCAGATTGAACCTAAATTTGTAGTGATCGATTCCATTCAGACGATTTTCAAGGAAGATATAACCTCTGC
CCCTGGTAGTGTGGCGCAGGTGAGAGAATGTACCAGTCACTTGATGCGAATTGCGAAAACGAAAGGTATTCCGATTTTCA
TTGTTGGACATGTTACTAAAGAAGGGTCCATTGCCGGCCCTCGTTTACTGGAACATATGGTGGATGCAGTTCTTTATTTT
GAGGGAGAGCGGCACCACACTTTCCGTATTCTCCGCAGTGTTAAAAACCGCTTCGGAAGTACACATGAAATGGGTATCTT
CGAGATGAAGGAGAAAGGGCTGGAAGAAGTACTTAATCCGTCGGAGATTTTCCTTGAAGAACGTTCTCAGGGAGCGGCAG
GCTCTATTGTTGTAGCTTCAATGGAGGGGACAAGGCCGGTGCTGGTAGAGATTCAGGCGCTCATATCCCCGACAGCCTAC
GGAAACCCCAGAAGGATGGCAACCGGTCTTGACACGAACAGAGTCCCTCTGCTCATGGCTGTGCTCGAGAAACGAGCAGG
ACTGTTGCTGCAGAATCAGGATGCGTACGTAAAGGTGGCGGGTGGTGTGAAGCTGGATGAGCCTGCAATCGACTTAGCTG
TTGCGTTAAGTATCGCATCGAGCTTTCGCAACAAAGTCTCAAACGGTGACGATGTCGTTGTTGGAGAAGTCGGATTGACA
GGAGAAATACGGCGGGTTTCCCGTATTGAACAGCGCGTACAGGAAGCGGCTAAACTCGGTTTCAAACGGGCCATCATACC
TAAGAAAAATATGGACGGATGGACTCCACCTTCACAAATTGAAGTCATCGGGGTAAGTACAGTACAGGAAGCAATGAAGG
TTACATTGGGGGAATCCTAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(64-222)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(363-430)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7Z2Y8A0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

72.429

99.564

0.721
  radA Streptococcus mitis NCTC 12261

62.914

98.693

0.621
  radA Streptococcus mitis SK321

62.914

98.693

0.621
  radA Streptococcus pneumoniae Rx1

62.693

98.693

0.619
  radA Streptococcus pneumoniae D39

62.693

98.693

0.619
  radA Streptococcus pneumoniae R6

62.693

98.693

0.619
  radA Streptococcus pneumoniae TIGR4

62.693

98.693

0.619

Multiple sequence alignment