Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   GSF71_RS01015 Genome accession   NZ_CP048116
Coordinates   214802..215509 (-) Length   235 a.a.
NCBI ID   WP_238389074.1    Uniprot ID   -
Organism   Latilactobacillus sakei strain MBEL1397     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 209802..220509
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GSF71_RS00990 rpsO 210096..210365 (-) 270 WP_011374766.1 30S ribosomal protein S15 -
  GSF71_RS00995 rpsT 210644..210898 (+) 255 WP_011374767.1 30S ribosomal protein S20 -
  GSF71_RS01000 holA 210967..211995 (-) 1029 WP_061826985.1 DNA polymerase III subunit delta -
  GSF71_RS01005 comEC 211992..214058 (-) 2067 WP_226474266.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  GSF71_RS01010 - 214256..214744 (-) 489 WP_011374770.1 ComE operon protein 2 -
  GSF71_RS01015 comEA 214802..215509 (-) 708 WP_238389074.1 helix-hairpin-helix domain-containing protein Machinery gene
  GSF71_RS01020 - 215583..216630 (-) 1048 Protein_203 SepM family pheromone-processing serine protease -
  GSF71_RS01025 coaD 216632..217129 (-) 498 WP_061826982.1 pantetheine-phosphate adenylyltransferase -
  GSF71_RS01030 rsmD 217142..217696 (-) 555 WP_011374774.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  GSF71_RS01035 - 217701..217991 (-) 291 WP_011374775.1 YlbG family protein -
  GSF71_RS01040 - 218015..219034 (-) 1020 WP_162100799.1 CAP-associated domain-containing protein -
  GSF71_RS01045 - 219113..220298 (-) 1186 Protein_208 FtsW/RodA/SpoVE family cell cycle protein -

Sequence

Protein


Download         Length: 235 a.a.        Molecular weight: 26112.72 Da        Isoelectric Point: 9.7683

>NTDB_id=419907 GSF71_RS01015 WP_238389074.1 214802..215509(-) (comEA) [Latilactobacillus sakei strain MBEL1397]
MMWQDYWQRYRRFWWVPCLVLLGIITGGFYWHQTQQKQKVTHNEQLLATSASKIKPATKKTHQAATKKKGGFVDIKGAVA
YSGIYPITVSETRLFEVLKNAGGPTDAADTKRLNLAQKLTDQLIIYIPKKGEVVTDQELIQAPQQIATTSERTPEQASTG
TSANASSASDTAKIDLNQADSTQLQTLNGIGPKKAEQIIAYRDEQGKFKQIEDLQKVGGIGPKTFEQLQSQICVN

Nucleotide


Download         Length: 708 bp        

>NTDB_id=419907 GSF71_RS01015 WP_238389074.1 214802..215509(-) (comEA) [Latilactobacillus sakei strain MBEL1397]
ATGATGTGGCAAGATTATTGGCAGCGATATCGGCGTTTTTGGTGGGTGCCTTGCTTAGTGCTATTGGGGATCATCACGGG
TGGCTTTTATTGGCATCAAACGCAACAAAAACAAAAAGTAACGCATAATGAGCAACTGCTAGCAACCAGTGCGTCTAAAA
TAAAACCTGCTACTAAGAAAACACACCAAGCGGCAACCAAGAAAAAAGGCGGTTTTGTCGATATAAAAGGTGCTGTGGCT
TATTCGGGAATTTATCCGATCACAGTCTCTGAAACGCGCTTATTTGAGGTGCTCAAAAATGCAGGTGGTCCGACTGATGC
GGCTGATACCAAGCGGCTTAATTTAGCCCAAAAATTAACCGATCAACTGATTATTTATATTCCGAAAAAAGGAGAGGTGG
TTACTGATCAAGAACTCATTCAAGCGCCACAACAAATTGCAACGACCAGTGAGCGTACGCCAGAACAAGCATCAACTGGG
ACAAGTGCGAATGCATCGAGCGCGAGTGACACTGCTAAAATCGATTTGAATCAGGCTGATAGTACACAGCTTCAGACCCT
CAATGGCATTGGCCCCAAGAAAGCTGAACAAATAATTGCTTATCGTGATGAACAAGGAAAATTTAAACAGATTGAAGATC
TACAAAAAGTCGGCGGGATTGGTCCGAAGACGTTTGAACAATTGCAATCACAAATTTGTGTGAATTAG

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(172-233)

SLBB domain (SLBB)

(73-124)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Latilactobacillus sakei subsp. sakei 23K

96.596

100

0.966

Multiple sequence alignment