Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   EBO27_RS12515 Genome accession   NZ_CP061034
Coordinates   2609042..2610769 (+) Length   575 a.a.
NCBI ID   WP_003113395.1    Uniprot ID   Q9I0G1
Organism   Pseudomonas aeruginosa strain PA3     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2604042..2615769
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EBO27_RS12495 (EBO27_12495) - 2604758..2605687 (-) 930 WP_150035180.1 LysR family transcriptional regulator -
  EBO27_RS12500 (EBO27_12500) - 2605710..2606683 (-) 974 Protein_2478 quinone oxidoreductase family protein -
  EBO27_RS12505 (EBO27_12505) - 2606909..2607661 (+) 753 WP_033994388.1 class I SAM-dependent methyltransferase -
  EBO27_RS12510 (EBO27_12510) - 2608249..2609052 (+) 804 WP_003097732.1 ABC transporter permease -
  EBO27_RS12515 (EBO27_12515) pilF 2609042..2610769 (+) 1728 WP_003113395.1 GspE/PulE family protein Machinery gene
  EBO27_RS12520 (EBO27_12520) - 2610774..2611961 (+) 1188 WP_023132666.1 type II secretion system F family protein -
  EBO27_RS12525 (EBO27_12525) gspG 2612015..2612449 (+) 435 WP_003110089.1 type II secretion system major pseudopilin GspG -
  EBO27_RS12530 (EBO27_12530) - 2612418..2612828 (+) 411 WP_003097723.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  EBO27_RS12535 (EBO27_12535) - 2612828..2613253 (+) 426 WP_003097721.1 type II secretion system protein J -
  EBO27_RS12540 (EBO27_12540) - 2613250..2613840 (+) 591 WP_003097720.1 type II secretion system protein J -
  EBO27_RS12545 (EBO27_12545) - 2613830..2614909 (+) 1080 WP_003160453.1 general secretion pathway protein GspK -

Sequence

Protein


Download         Length: 575 a.a.        Molecular weight: 63727.82 Da        Isoelectric Point: 5.2438

>NTDB_id=419670 EBO27_RS12515 WP_003113395.1 2609042..2610769(+) (pilF) [Pseudomonas aeruginosa strain PA3]
MSSEAVSPGPQMTAEWIPLGQRLLERGLVSGQELERALDLQRRLGGRLGGILVRSGAISENTLMQVLAEQLRLPLVGDDL
RKPSEESIAAFLLSTPINTDWFVEEQLVVWEEGEQLLFAARDPLSPSIRETLGYFYPERSIQAVLCRSQDLDGWLEHSLD
LARQGADRHAYGSEDIRYLRELAEEAPVVELVNNVMSQAMEKRSSDIHIEPREFDFRIRFRVDGVLHDQLSLPRERFAAV
VSRIKLISAIDIAERRLPQDGRMSIRVSGTELDVRVSTLPGVHGESVVMRLLPKEREGLRLDRLGMLPDHLRRMRAWVAE
PHGIILVTGPTGSGKSTTLYGALEEINDGVRKLISVEDPVEYQVPNITQVQVHADIGLTFAAALRSILRQDPDVIMIGEI
RDLETAEIAVQSSLTGHLVLSTLHTNDAVSAFTRLIDMGVEPFLVASPVRGVQAQRLVRRLCAHCAEPCEPTLAAADLAA
TEAATARLFPGQAAQWRVARGCGRCEGTGYSGRVGIYELVDLSSEMRDLIVQRASLEQMHRLAASQGQRNLREDGLIKAW
LGMTSLDEVHRVTSG

Nucleotide


Download         Length: 1728 bp        

>NTDB_id=419670 EBO27_RS12515 WP_003113395.1 2609042..2610769(+) (pilF) [Pseudomonas aeruginosa strain PA3]
ATGTCCTCTGAGGCCGTTTCCCCGGGCCCGCAGATGACGGCCGAGTGGATACCGCTGGGGCAGCGTTTGCTCGAACGCGG
GTTGGTGAGCGGCCAGGAACTCGAGCGGGCGCTCGACCTGCAACGCCGGCTTGGCGGCCGTCTGGGAGGCATTCTCGTGC
GTTCCGGAGCCATCTCGGAAAATACCCTCATGCAGGTGCTGGCGGAGCAACTGCGGTTGCCGCTGGTGGGGGACGACCTG
CGGAAGCCGAGCGAAGAGTCCATCGCCGCATTCCTGCTCAGTACCCCGATCAATACCGACTGGTTCGTCGAAGAGCAACT
GGTGGTCTGGGAGGAGGGCGAGCAACTGCTGTTCGCGGCGCGTGATCCGTTATCTCCTTCGATACGCGAAACCCTGGGCT
ATTTCTATCCGGAGCGCTCGATTCAAGCAGTGCTGTGCCGCTCCCAGGATCTGGACGGCTGGTTGGAGCACTCCCTGGAC
CTGGCCCGGCAAGGTGCGGATCGGCACGCCTACGGCAGCGAAGACATCCGCTACCTGCGGGAGTTGGCGGAGGAGGCGCC
GGTAGTCGAACTCGTCAACAACGTGATGAGCCAGGCCATGGAAAAGCGCTCTTCGGATATTCACATCGAACCCCGGGAGT
TCGACTTCCGTATCCGCTTCCGCGTCGACGGCGTCCTGCACGACCAACTGAGCCTTCCCCGCGAGCGATTCGCGGCGGTT
GTCTCGCGGATCAAGCTGATCTCCGCCATCGACATCGCGGAGCGACGGTTGCCGCAGGATGGACGCATGAGCATCCGGGT
CAGCGGTACGGAGCTCGACGTGCGGGTCTCCACCTTGCCAGGGGTGCATGGCGAATCGGTGGTCATGCGCCTGTTGCCGA
AGGAGCGCGAGGGGTTGCGCCTGGACCGGCTCGGGATGCTCCCGGACCACTTGCGGAGGATGCGTGCATGGGTAGCGGAA
CCCCACGGCATCATCCTGGTGACCGGCCCCACCGGATCGGGCAAGTCCACCACGCTCTATGGCGCGCTGGAGGAAATCAA
TGATGGCGTGCGCAAGCTCATCAGCGTGGAAGATCCCGTGGAATACCAGGTGCCCAATATCACCCAGGTCCAGGTCCATG
CCGACATCGGCCTGACCTTCGCTGCCGCCTTGCGCTCGATCCTGCGGCAGGATCCCGATGTGATCATGATCGGGGAGATA
CGCGACCTGGAGACGGCGGAGATCGCCGTACAGTCCTCGCTCACCGGTCACCTGGTGCTTTCGACCCTGCATACCAACGA
TGCGGTGAGCGCCTTCACGCGCTTGATCGACATGGGCGTGGAACCCTTCCTGGTGGCTTCGCCGGTGCGCGGTGTCCAGG
CCCAGCGACTGGTGCGCAGGTTGTGCGCCCACTGCGCGGAACCTTGCGAGCCAACCCTTGCTGCGGCCGATCTGGCAGCG
ACCGAGGCGGCCACCGCGCGGCTGTTCCCGGGGCAGGCAGCGCAATGGCGCGTGGCGCGAGGCTGCGGCCGCTGCGAAGG
TACCGGCTACAGCGGCCGTGTGGGCATCTACGAACTCGTCGATCTGTCTTCCGAGATGCGCGACCTGATCGTGCAGAGAG
CTTCCCTCGAACAGATGCATCGGCTTGCCGCCAGCCAGGGACAGCGCAATCTGCGTGAAGATGGCTTGATCAAGGCCTGG
TTGGGTATGACCAGTCTTGACGAGGTGCATCGCGTGACCAGCGGCTAA

Domains

Predicted by InterProScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(52-160)

Type II/IV secretion system protein (T2SSE)

(194-460)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q9I0G1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Thermus thermophilus HB27

41.696

98.435

0.41
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.801

98.609

0.383
  pilF Neisseria gonorrhoeae MS11

36.842

99.13

0.365