Detailed information    

insolico Bioinformatically predicted

Overview

Name   pepF   Type   Regulator
Locus tag   GWK37_RS14960 Genome accession   NZ_CP048002
Coordinates   3006317..3008146 (+) Length   609 a.a.
NCBI ID   WP_115940977.1    Uniprot ID   -
Organism   Bacillus velezensis strain CACC 316     
Function   degradation of XIP; competence shut-off (predicted from homology)   
Competence regulation

Genomic Context

Location: 3001317..3013146
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GWK37_RS14935 (GWK37_14945) - 3001979..3002557 (+) 579 WP_007409110.1 GNAT family N-acetyltransferase -
  GWK37_RS14940 (GWK37_14950) spx 3002736..3003131 (+) 396 WP_003155034.1 transcriptional regulator Spx -
  GWK37_RS14945 (GWK37_14955) - 3003189..3003845 (-) 657 WP_014417421.1 TerC family protein -
  GWK37_RS14950 (GWK37_14960) mecA 3004121..3004777 (+) 657 WP_003155032.1 adaptor protein MecA Regulator
  GWK37_RS14955 (GWK37_14965) - 3004929..3006089 (+) 1161 WP_094295680.1 competence protein CoiA family protein -
  GWK37_RS14960 (GWK37_14970) pepF 3006317..3008146 (+) 1830 WP_115940977.1 oligoendopeptidase F Regulator
  GWK37_RS19640 - 3008184..3008351 (-) 168 WP_003155026.1 hypothetical protein -
  GWK37_RS14965 (GWK37_14975) spxH 3008637..3009539 (-) 903 WP_115940904.1 protease adaptor protein SpxH -
  GWK37_RS14970 (GWK37_14980) - 3009536..3009934 (-) 399 WP_115940903.1 thiol management oxidoreductase -
  GWK37_RS14975 (GWK37_14985) - 3010163..3010849 (-) 687 WP_115940902.1 lytic transglycosylase domain-containing protein -
  GWK37_RS14980 (GWK37_14990) - 3010854..3011426 (-) 573 WP_115940901.1 CYTH domain-containing protein -
  GWK37_RS14985 (GWK37_14995) - 3011551..3011916 (+) 366 WP_012117290.1 hypothetical protein -
  GWK37_RS14990 (GWK37_15000) yjbM 3011944..3012579 (+) 636 WP_003155019.1 GTP diphosphokinase -

Sequence

Protein


Download         Length: 609 a.a.        Molecular weight: 69785.49 Da        Isoelectric Point: 5.3508

>NTDB_id=419259 GWK37_RS14960 WP_115940977.1 3006317..3008146(+) (pepF) [Bacillus velezensis strain CACC 316]
MTKENKAAQLPDRSEVKAEHTWRLEDIFPSDEAWNKEFQAVKELIPNLSSFKGTLAHSADNLYAALTAQDEVTNRLGKLY
AYAHMRSDQDTGNSFYQGLNDKAASLYSQAASATAYIEPEILAIQEDKLHQFILEKEELKLYSHAIEEITKQRPHVLSEQ
EEALLAEASEALSSPSNTFSVLNNADITFPSIKDENGKETQITHGNFINFLESSNREVRKNAFEAVYKTYGQYKNTMATT
LSGTVKKDNFYARVKKYKSAREAALSNNSIPEEVYDNLIKTINKHLPLLHRYIDLRKKVLGLDEVHIYDLYTPLVKDSGM
KVTYEEAKDYMLKGLAPLGEEYASILKEGLENRWVDVYENKGKRSGAYSSGTYGTNPYILMNWHDNVNNLFTLVHEFGHS
VHSYYTRKYQPYPYGNYSIFVAEVASTTNEALLGEYMLNSLKDEKQRLYLLNHMLEGFRGTVFRQTMFAEFEHLIHTKAQ
EGEPLTPELLNELYYDLNKKYFGDTMVIDKEISLEWSRIPHFYYNYYVYQYATGYSAAQALSSQILKEGKPAVERYTEFL
KAGSSEYPIDILKKAGVDMTSPEPIEAACKMFEEKLNEMEELLSKVNPS

Nucleotide


Download         Length: 1830 bp        

>NTDB_id=419259 GWK37_RS14960 WP_115940977.1 3006317..3008146(+) (pepF) [Bacillus velezensis strain CACC 316]
ATGACTAAGGAAAATAAAGCAGCACAGCTGCCTGACAGAAGTGAAGTAAAGGCAGAGCATACATGGAGACTAGAGGATAT
TTTTCCTAGTGATGAGGCCTGGAATAAAGAATTTCAAGCAGTTAAAGAATTAATCCCGAATTTATCTTCTTTTAAAGGCA
CGCTTGCTCATTCGGCTGATAATTTATACGCGGCGCTTACGGCACAGGATGAAGTGACGAACCGCCTTGGGAAACTTTAT
GCATACGCGCATATGCGCTCAGACCAGGATACGGGAAATTCTTTCTACCAAGGGCTGAACGACAAAGCCGCAAGCCTGTA
TTCCCAGGCTGCCAGCGCGACTGCTTACATTGAACCGGAAATTCTGGCGATTCAGGAGGACAAGCTGCACCAGTTCATTC
TGGAAAAAGAAGAGCTTAAGCTTTACTCCCATGCAATTGAAGAAATTACAAAACAGCGTCCGCACGTGCTGAGCGAACAA
GAAGAGGCCCTGCTTGCGGAAGCCTCAGAAGCGCTGTCGTCGCCTTCCAATACATTCAGTGTTTTAAATAACGCCGATAT
TACGTTTCCGTCGATTAAAGATGAAAACGGCAAGGAAACTCAAATCACACACGGCAACTTTATTAATTTCCTGGAAAGCA
GCAATCGTGAAGTCCGCAAAAACGCATTTGAAGCCGTATATAAAACATACGGACAGTATAAAAACACGATGGCGACTACA
CTCAGCGGCACTGTGAAAAAAGATAATTTTTACGCCCGTGTGAAAAAATATAAATCAGCACGGGAAGCGGCTTTATCCAA
TAACAGCATCCCTGAAGAAGTTTACGATAACCTGATTAAAACGATCAATAAACATCTTCCGTTATTACACCGTTACATTG
ACCTGCGGAAAAAGGTTCTCGGCCTTGATGAAGTCCATATTTATGACCTGTACACACCCCTTGTAAAAGATTCGGGAATG
AAGGTCACATATGAGGAAGCGAAAGATTATATGCTCAAAGGACTTGCTCCGCTGGGCGAGGAATACGCGTCTATTTTAAA
AGAAGGACTTGAAAACCGCTGGGTCGATGTATACGAAAACAAAGGAAAACGCAGCGGTGCTTATTCGTCCGGTACATACG
GCACGAATCCGTACATCTTGATGAACTGGCATGACAATGTCAATAATCTGTTTACGCTCGTTCACGAGTTCGGCCATTCC
GTGCACAGCTACTATACGAGAAAATATCAGCCGTATCCATACGGTAACTACAGCATTTTTGTCGCTGAAGTCGCTTCAAC
GACCAATGAAGCGCTGCTCGGTGAATATATGCTGAACAGCTTAAAGGATGAAAAACAGCGTCTGTACCTGCTCAACCACA
TGCTTGAAGGATTCAGGGGCACAGTGTTCAGACAGACGATGTTCGCTGAATTTGAGCATCTGATTCATACGAAAGCGCAG
GAAGGCGAGCCGCTGACGCCTGAACTGCTGAACGAACTTTATTATGATCTGAATAAAAAGTATTTCGGTGACACCATGGT
GATTGATAAAGAAATCAGTCTGGAATGGTCAAGAATTCCGCATTTCTATTACAATTATTATGTGTATCAATATGCGACAG
GCTACAGTGCGGCCCAAGCGCTGAGCAGCCAGATCTTAAAAGAAGGCAAGCCTGCGGTGGAGCGCTATACCGAATTCTTA
AAAGCGGGAAGCTCGGAATATCCGATCGACATACTGAAAAAAGCGGGAGTCGATATGACATCGCCTGAACCGATCGAGGC
GGCCTGCAAAATGTTTGAAGAGAAACTGAATGAAATGGAAGAACTTCTTTCGAAAGTGAACCCTTCGTAA

Domains

Predicted by InterproScan.

Peptidase family M3 (Peptidase_M3)

(211-590)

Oligopeptidase F (Peptidase_M3_N)

(120-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pepF Streptococcus salivarius strain HSISS4

50

97.537

0.488

Multiple sequence alignment