Detailed information    

insolico Bioinformatically predicted

Overview

Name   ccrA   Type   Machinery gene
Locus tag   GWG04_RS10255 Genome accession   NZ_CP047922
Coordinates   2056142..2057491 (+) Length   449 a.a.
NCBI ID   WP_000815638.1    Uniprot ID   Q53IL8
Organism   Staphylococcus aureus strain SR231     
Function   promote SCCmec transfer (predicted from homology)   
Homologous recombination

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
SCCmec 2050969..2080996 2056142..2057491 within 0


Gene organization within MGE regions


Location: 2050969..2080996
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GWG04_RS10235 (GWG04_10250) - 2050969..2052123 (-) 1155 WP_000912116.1 ATP-binding protein -
  GWG04_RS10240 (GWG04_10255) - 2052580..2053626 (+) 1047 WP_001042023.1 hypothetical protein -
  GWG04_RS10245 (GWG04_10260) - 2053819..2054115 (+) 297 WP_000273823.1 single-stranded DNA-binding protein -
  GWG04_RS10250 (GWG04_10265) - 2054115..2055908 (+) 1794 WP_000079473.1 DUF927 domain-containing protein -
  GWG04_RS10255 (GWG04_10270) ccrA 2056142..2057491 (+) 1350 WP_000815638.1 recombinase family protein Machinery gene
  GWG04_RS10260 (GWG04_10275) ccrB 2057513..2059141 (+) 1629 WP_103147903.1 recombinase family protein Machinery gene
  GWG04_RS10265 (GWG04_10280) - 2059507..2059938 (-) 432 Protein_2000 hypothetical protein -
  GWG04_RS10270 (GWG04_10285) - 2060097..2061701 (+) 1605 WP_103147904.1 IS1182-like element IS1182 family transposase -
  GWG04_RS10275 (GWG04_10295) - 2062422..2063081 (-) 660 WP_000802831.1 RibD family protein -
  GWG04_RS10280 (GWG04_10300) - 2063192..2063548 (-) 357 WP_000374054.1 WYL domain-containing protein -
  GWG04_RS10285 (GWG04_10310) - 2063572..2064144 (-) 573 WP_001079938.1 HTH domain-containing protein -
  GWG04_RS10290 (GWG04_10320) aph(3')-IIIa 2064420..2065214 (-) 795 WP_001096887.1 aminoglycoside O-phosphotransferase APH(3')-IIIa -
  GWG04_RS10295 (GWG04_10325) sat4 2065307..2065849 (-) 543 WP_000627290.1 streptothricin N-acetyltransferase Sat4 -
  GWG04_RS10300 (GWG04_10330) - 2065846..2066754 (-) 909 WP_001255866.1 aminoglycoside nucleotidyltransferase ANT(6)-Ia -
  GWG04_RS10305 (GWG04_10335) - 2066787..2067521 (-) 735 WP_000662263.1 class I SAM-dependent methyltransferase -
  GWG04_RS10310 (GWG04_10340) - 2067502..2068371 (-) 870 WP_000228166.1 nucleotidyltransferase domain-containing protein -
  GWG04_RS10315 (GWG04_10345) - 2068474..2068719 (-) 246 WP_000567888.1 single-stranded DNA-binding protein -
  GWG04_RS10320 (GWG04_10350) - 2068846..2070450 (-) 1605 WP_103147904.1 IS1182-like element IS1182 family transposase -
  GWG04_RS10325 (GWG04_10355) - 2070734..2071315 (-) 582 WP_001124173.1 TetR family transcriptional regulator C-terminal domain-containing protein -
  GWG04_RS10330 (GWG04_10360) - 2071521..2072114 (+) 594 WP_000690628.1 recombinase family protein -
  GWG04_RS10335 (GWG04_10365) - 2072086..2073528 (+) 1443 WP_103147960.1 Mu transposase C-terminal domain-containing protein -
  GWG04_RS10340 (GWG04_10370) - 2073521..2074336 (+) 816 WP_000164963.1 AAA family ATPase -
  GWG04_RS10345 (GWG04_10375) - 2074753..2075103 (+) 351 WP_000849672.1 SAUGI family uracil-DNA glycosylase inhibitor -
  GWG04_RS10350 (GWG04_10385) - 2075190..2075501 (+) 312 WP_000700851.1 DUF960 family protein -
  GWG04_RS10355 (GWG04_10390) - 2075516..2076022 (+) 507 WP_001092189.1 DUF1643 domain-containing protein -
  GWG04_RS10360 (GWG04_10395) - 2076074..2077681 (+) 1608 Protein_2019 IS1182 family transposase -
  GWG04_RS10365 (GWG04_10400) - 2077672..2077932 (-) 261 WP_001549961.1 type I restriction enzyme endonuclease domain-containing protein -
  GWG04_RS10370 (GWG04_10405) - 2077904..2078890 (-) 987 WP_001549960.1 beta-lactam sensor/signal transducer MecR1 -
  GWG04_RS10375 (GWG04_10410) mecA 2078987..2080996 (+) 2010 WP_063852655.1 PBP2a family beta-lactam-resistant peptidoglycan transpeptidase MecA -

Sequence

Protein


Download         Length: 449 a.a.        Molecular weight: 52636.23 Da        Isoelectric Point: 9.9639

>NTDB_id=418427 GWG04_RS10255 WP_000815638.1 2056142..2057491(+) (ccrA) [Staphylococcus aureus strain SR231]
MKQVIGYLRQSTMKQQSLAAQKQAIEAIAEKHHIQHINFYSDKQSGRKDNRSGYRQITQLIQQGQCDILCCYRLNRLHRN
LKNALKLIKLCQTYHVHILSVHDGYFDMDQAFDRLKLNIFISLAELESDNIGEQVRNGLQEKAKQGRLITTHAPFGYDYH
HGTFIINQNESPTVKAVFNYYIKGHGYKKIAQLLEEDNTYINRQPYQVRNIIINPNYCGRVNNQYGQFDNMFPSIVSTSI
YEQAQRLRSQKQTKQTSSDNQLKQKIKCPCCNATLTNMTIRKKNHTLRYYVCPKNMNASRFVCDFKGINAQTLEDKVLEV
CRDFYQNQRIYTKIKSAIDKRIKRQRNIEKHHTLTQEQLIEKLAQGIIDAETFREQTQSLRQQPQRTTSINGHQIQHIIQ
NIIQKRFTLNILYPYIETIHITKDKNLIGIYFKNEPLNIVNQTMQSSIA

Nucleotide


Download         Length: 1350 bp        

>NTDB_id=418427 GWG04_RS10255 WP_000815638.1 2056142..2057491(+) (ccrA) [Staphylococcus aureus strain SR231]
ATGAAACAAGTCATAGGCTATTTACGTCAAAGTACGATGAAACAACAATCTCTTGCAGCACAGAAACAAGCTATCGAAGC
AATAGCCGAAAAACATCATATTCAACATATCAACTTTTATAGCGACAAACAATCAGGTCGCAAAGATAATCGTAGTGGGT
ATCGACAAATAACACAATTAATTCAACAAGGACAGTGTGACATATTATGCTGTTATCGTCTTAATAGGTTGCATCGTAAT
CTGAAAAATGCATTAAAACTCATCAAATTATGTCAAACGTACCATGTTCATATCTTAAGCGTACATGATGGTTACTTTGA
TATGGATCAAGCTTTCGACCGACTCAAGCTTAATATCTTCATCAGTTTAGCCGAACTTGAATCGGATAACATTGGAGAAC
AAGTCAGAAATGGGCTTCAAGAAAAAGCAAAGCAAGGTCGATTGATTACAACACATGCACCTTTTGGTTACGATTATCAC
CACGGAACATTCATCATCAATCAAAATGAGTCACCAACGGTAAAGGCTGTATTCAATTATTACATTAAAGGCCATGGTTA
TAAGAAAATTGCACAGTTATTAGAAGAAGATAACACGTATATCAATCGACAACCCTATCAAGTTCGTAACATTATCATCA
ATCCTAATTATTGTGGTCGTGTCAACAATCAATATGGCCAATTCGACAATATGTTTCCTTCTATTGTTTCCACAAGTATA
TATGAGCAAGCGCAGAGACTTCGATCGCAAAAACAAACCAAACAGACATCTTCGGATAATCAACTCAAACAAAAAATCAA
ATGCCCATGTTGTAATGCAACACTTACAAATATGACCATTAGAAAAAAGAATCATACATTACGTTACTACGTCTGTCCTA
AAAACATGAATGCTTCACGCTTTGTCTGTGATTTTAAAGGCATCAATGCACAAACACTTGAAGATAAAGTATTAGAAGTG
TGCCGAGACTTTTATCAAAATCAACGCATCTACACAAAAATTAAAAGTGCGATTGACAAACGCATCAAAAGACAAAGAAA
CATAGAAAAACATCACACATTGACTCAAGAACAACTGATAGAAAAGTTGGCACAAGGCATCATTGATGCAGAAACGTTCA
GAGAGCAAACACAATCATTACGTCAACAACCGCAACGCACTACATCTATCAATGGGCATCAAATACAACACATCATTCAA
AATATTATTCAAAAACGTTTCACGTTAAACATATTGTACCCCTATATTGAAACCATTCACATTACGAAAGATAAAAATCT
TATAGGAATCTATTTCAAAAATGAACCACTCAATATTGTCAATCAAACCATGCAATCATCGATTGCATAA

Domains

Predicted by InterproScan.

Resolvase, N terminal domain (Resolvase)

(4-147)

Recombinase (Recombinase)

(169-250)

Recombinase zinc beta ribbon domain (Zn_ribbon_recom)

(262-321)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q53IL8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ccrA Staphylococcus aureus N315

97.55

100

0.976
  ccrA Staphylococcus aureus COL

75.501

100

0.755

Multiple sequence alignment