Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   M594_RS00130 Genome accession   NZ_CP047883
Coordinates   24078..25439 (+) Length   453 a.a.
NCBI ID   WP_254597275.1    Uniprot ID   -
Organism   Streptococcus mitis strain S022-V3-A4     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 19078..30439
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M594_RS00100 (M594_00100) comW 20498..20734 (+) 237 WP_173875631.1 sigma(X)-activator ComW Regulator
  M594_RS00105 (M594_00105) - 20965..22251 (+) 1287 WP_001832534.1 adenylosuccinate synthase -
  M594_RS00110 (M594_00110) tadA 22450..22917 (+) 468 WP_001110110.1 tRNA adenosine(34) deaminase TadA -
  M594_RS00120 (M594_00120) - 23104..23547 (+) 444 WP_000701987.1 dUTP diphosphatase -
  M594_RS00125 (M594_00125) - 23507..24064 (+) 558 WP_173875632.1 histidine phosphatase family protein -
  M594_RS00130 (M594_00130) radA 24078..25439 (+) 1362 WP_254597275.1 DNA repair protein RadA Machinery gene
  M594_RS00135 (M594_00135) - 25512..26009 (+) 498 WP_173875633.1 beta-class carbonic anhydrase -
  M594_RS00140 (M594_00140) - 26034..26849 (+) 816 WP_173875634.1 PrsW family intramembrane metalloprotease -
  M594_RS00145 (M594_00145) - 26994..27962 (+) 969 WP_000010168.1 ribose-phosphate diphosphokinase -
  M594_RS00150 (M594_00150) - 28080..28517 (+) 438 WP_173875635.1 CoA-binding protein -
  M594_RS00155 (M594_00155) - 28549..29559 (-) 1011 WP_173875636.1 YeiH family protein -

Sequence

Protein


Download         Length: 453 a.a.        Molecular weight: 49415.92 Da        Isoelectric Point: 6.8558

>NTDB_id=418221 M594_RS00130 WP_254597275.1 24078..25439(+) (radA) [Streptococcus mitis strain S022-V3-A4]
MAKKKATFVCQNCGYNSPKYLGRCPNCGSWSSFVEEVEVAEVKNARVSLTGEKTKPMKLAEVTSINVNRTKTDMEEFNRV
LGGGVVPGSLVLIGGDPGIGKSTLLLQVSTQLSQVGTVLYISGEESAQQIKLRAERLGDIDSEFYLYAETNMQSVRAEVE
RIQPNFLIIDSIQTIMSPEISGVQGSVSQVREVTAELMQLAKTNNIAIFIVGHVTKEGTLAGPRMLEHMVDTVLYFEGER
HHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLNPSQVFLEERLDGATGSSIVVTMEGTRPILAEVQALVTPTMFGNAK
RTTTGLDFNRASLIMAVLEKRAGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKDKPTNPQECFVGELGLTGEIR
RVNRIEQRINEAAKLGFTKIYVPKNSLTGITPPKEIQVIGVTTIQEVLKKVFA

Nucleotide


Download         Length: 1362 bp        

>NTDB_id=418221 M594_RS00130 WP_254597275.1 24078..25439(+) (radA) [Streptococcus mitis strain S022-V3-A4]
ATCGCAAAGAAAAAAGCGACATTTGTATGTCAAAATTGTGGGTATAATTCCCCTAAATATCTGGGACGTTGCCCCAACTG
TGGGTCTTGGTCTTCTTTTGTAGAAGAGGTTGAGGTTGCCGAGGTAAAGAATGCGCGTGTGTCCTTGACAGGTGAGAAAA
CCAAGCCCATGAAACTGGCTGAGGTGACTTCTATCAACGTCAATCGAACCAAGACGGACATGGAGGAATTCAACCGTGTA
CTTGGAGGCGGAGTGGTACCGGGAAGTCTCGTTCTTATCGGTGGGGATCCTGGAATCGGGAAATCAACCCTTCTCCTACA
AGTCTCAACCCAGTTGTCCCAAGTGGGAACGGTTCTCTATATCAGTGGTGAGGAGTCTGCCCAGCAAATCAAGTTGCGAG
CGGAACGCTTGGGAGATATTGATAGCGAGTTTTATCTCTATGCAGAGACCAATATGCAGAGTGTTCGAGCTGAGGTAGAA
AGAATCCAGCCAAACTTCCTCATTATTGACTCCATTCAGACCATTATGTCTCCTGAGATTTCAGGGGTGCAGGGGTCTGT
TTCTCAAGTGCGTGAGGTGACAGCTGAACTTATGCAGCTGGCCAAAACTAATAACATTGCCATCTTTATCGTAGGTCATG
TGACCAAGGAAGGAACCTTGGCTGGGCCTCGTATGTTGGAGCATATGGTGGATACGGTGCTTTACTTTGAAGGGGAGCGT
CACCACACCTTCCGTATTTTAAGAGCGGTCAAAAACCGTTTTGGCTCCACTAATGAGATTGGGATTTTTGAGATGCAGTC
GGGCGGATTGGTTGAGGTACTCAATCCGAGTCAAGTTTTCCTAGAAGAGCGTTTGGATGGGGCGACTGGTTCGTCAATCG
TTGTGACCATGGAAGGGACGCGTCCGATTTTGGCGGAGGTTCAGGCCTTGGTGACACCGACCATGTTTGGAAATGCCAAG
CGTACTACGACAGGACTTGATTTTAACCGTGCTAGCTTGATTATGGCTGTTTTGGAAAAGCGTGCAGGGCTTCTCTTGCA
AAATCAGGATGCCTATCTCAAATCTGCTGGCGGTGTTAAATTGGATGAACCTGCTATAGACTTGGCCGTTGCAGTTGCCA
TTGCTTCTAGTTATAAAGACAAGCCAACCAATCCTCAGGAATGTTTTGTAGGCGAACTGGGCTTGACAGGAGAGATTCGG
CGCGTGAATCGTATCGAGCAACGCATCAACGAAGCTGCTAAACTGGGATTTACCAAGATTTATGTACCAAAAAATTCCTT
GACAGGAATCACTCCGCCTAAGGAAATTCAGGTCATTGGTGTGACAACGATTCAGGAAGTCTTGAAAAAGGTCTTTGCAT
AA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(64-218)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(360-425)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis SK321

99.117

100

0.991
  radA Streptococcus mitis NCTC 12261

99.117

100

0.991
  radA Streptococcus pneumoniae Rx1

99.117

100

0.991
  radA Streptococcus pneumoniae D39

99.117

100

0.991
  radA Streptococcus pneumoniae R6

99.117

100

0.991
  radA Streptococcus pneumoniae TIGR4

99.117

100

0.991
  radA Bacillus subtilis subsp. subtilis str. 168

63.355

100

0.634

Multiple sequence alignment