Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   E3S71_RS02445 Genome accession   NZ_CP047852
Coordinates   455474..455971 (+) Length   165 a.a.
NCBI ID   WP_029694076.1    Uniprot ID   -
Organism   Staphylococcus aureus strain UP_274     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 450474..460971
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E3S71_RS02405 (E3S71_02405) - 450518..451504 (+) 987 WP_000161315.1 ROK family glucokinase -
  E3S71_RS02410 (E3S71_02410) - 451504..451833 (+) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  E3S71_RS02415 (E3S71_02415) - 451830..452453 (+) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  E3S71_RS02420 (E3S71_02420) comGA 452505..453479 (+) 975 WP_000697235.1 competence type IV pilus ATPase ComGA Machinery gene
  E3S71_RS02425 (E3S71_02425) comGB 453451..454521 (+) 1071 WP_000776422.1 competence type IV pilus assembly protein ComGB Machinery gene
  E3S71_RS02430 (E3S71_02430) comGC 454535..454846 (+) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  E3S71_RS02435 (E3S71_02435) comGD 454824..455270 (+) 447 WP_072433556.1 competence type IV pilus minor pilin ComGD Machinery gene
  E3S71_RS02440 (E3S71_02440) comGE 455257..455556 (+) 300 WP_000844413.1 hypothetical protein Machinery gene
  E3S71_RS02445 (E3S71_02445) comGF 455474..455971 (+) 498 WP_029694076.1 competence type IV pilus minor pilin ComGF Machinery gene
  E3S71_RS02450 (E3S71_02450) - 456068..456214 (+) 147 WP_001789879.1 hypothetical protein -
  E3S71_RS02455 (E3S71_02455) - 456204..456728 (+) 525 WP_001015121.1 shikimate kinase -
  E3S71_RS02460 (E3S71_02460) gcvT 456887..457978 (+) 1092 WP_160199867.1 glycine cleavage system aminomethyltransferase GcvT -
  E3S71_RS02465 (E3S71_02465) gcvPA 457998..459344 (+) 1347 WP_000019678.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  E3S71_RS02470 (E3S71_02470) gcvPB 459337..460809 (+) 1473 WP_000202188.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPB -

Sequence

Protein


Download         Length: 165 a.a.        Molecular weight: 19183.92 Da        Isoelectric Point: 10.2877

>NTDB_id=417845 E3S71_RS02445 WP_029694076.1 455474..455971(+) (comGF) [Staphylococcus aureus strain UP_274]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISITLLIVPDLIRLSKTFLIESRELTTVDFEFFSRDI
LEDFKGVDRNDIELRQQRIILHKGEEMIEYKLINNKIIKVVNDRGNITMINNVTSFTANIYYKSIIKITITVKVGTNVQT
KTIFV

Nucleotide


Download         Length: 498 bp        

>NTDB_id=417845 E3S71_RS02445 WP_029694076.1 455474..455971(+) (comGF) [Staphylococcus aureus strain UP_274]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTCATTGAAATGTTAGTAGCGATGATGGTTATAAGTATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAGCAAAACTTTTCTAATTGAAAGTAGGGAATTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGAGGATTTTAAAGGAGTAGATAGAAACGATATTGAACTTAGGCAACAGCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATAAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGATTAATAATGTTA
CTTCATTTACTGCAAATATCTACTATAAATCCATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATGTGCAGACT
AAAACTATTTTTGTATAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(68-155)

Prokaryotic N-terminal methylation motif (N_methyl)

(28-51)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Staphylococcus aureus MW2

96.364

100

0.964
  comGF Staphylococcus aureus N315

96.364

100

0.964

Multiple sequence alignment