Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   E3S74_RS08135 Genome accession   NZ_CP047851
Coordinates   1694795..1695481 (-) Length   228 a.a.
NCBI ID   WP_001794049.1    Uniprot ID   A0A0E1X4U4
Organism   Staphylococcus aureus strain UP_338     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1689795..1700481
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E3S74_RS08115 (E3S74_08115) rpsT 1690709..1690960 (+) 252 WP_001274017.1 30S ribosomal protein S20 -
  E3S74_RS08120 (E3S74_08120) holA 1691005..1691979 (-) 975 WP_001282570.1 DNA polymerase III subunit delta -
  E3S74_RS08125 (E3S74_08125) comEC 1692036..1694237 (-) 2202 WP_196777102.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  E3S74_RS08130 (E3S74_08130) - 1694242..1694703 (-) 462 WP_000439692.1 ComE operon protein 2 -
  E3S74_RS08135 (E3S74_08135) comEA 1694795..1695481 (-) 687 WP_001794049.1 ComEA family DNA-binding protein Machinery gene
  E3S74_RS08140 (E3S74_08140) - 1695521..1696237 (-) 717 WP_000084835.1 class I SAM-dependent DNA methyltransferase -
  E3S74_RS08145 (E3S74_08145) rsfS 1696240..1696593 (-) 354 WP_001088020.1 ribosome silencing factor -
  E3S74_RS08150 (E3S74_08150) yqeK 1696594..1697178 (-) 585 WP_001019324.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  E3S74_RS08155 (E3S74_08155) nadD 1697168..1697737 (-) 570 WP_000822938.1 nicotinate (nicotinamide) nucleotide adenylyltransferase -
  E3S74_RS08160 (E3S74_08160) yhbY 1697740..1698030 (-) 291 WP_000955232.1 ribosome assembly RNA-binding protein YhbY -
  E3S74_RS08165 (E3S74_08165) aroE 1698034..1698840 (-) 807 WP_000666750.1 shikimate dehydrogenase -
  E3S74_RS08170 (E3S74_08170) yqeH 1698854..1699954 (-) 1101 WP_001280134.1 ribosome biogenesis GTPase YqeH -

Sequence

Protein


Download         Length: 228 a.a.        Molecular weight: 25766.38 Da        Isoelectric Point: 9.3383

>NTDB_id=417812 E3S74_RS08135 WP_001794049.1 1694795..1695481(-) (comEA) [Staphylococcus aureus strain UP_338]
MVLLYQFLLRYKDFLAQWKLYIISAVVLIMVLIGFIFWRQDDYTSRDFENKDTVLKQSTSENSSLSKLEDVQVKDGDNSK
NKGPVYVDIKGAVKHPNVYKMTSKDRVVDLLDKAQLLDDADVSQINLSEKLTDQKMIFIPHKGQKNVEPQFGANSVHVKN
GNTNNTKVNLNTASVSELMSVPGVGQAKANAIVEYRNQQGAFQEIDDLKKVKGFGSKTFDKLNSYFTT

Nucleotide


Download         Length: 687 bp        

>NTDB_id=417812 E3S74_RS08135 WP_001794049.1 1694795..1695481(-) (comEA) [Staphylococcus aureus strain UP_338]
GTGGTTTTATTGTATCAATTTTTATTACGCTATAAAGATTTTTTAGCTCAATGGAAGTTATATATTATAAGTGCTGTTGT
TTTAATTATGGTATTAATTGGTTTTATATTCTGGAGACAAGATGATTATACTTCAAGAGATTTTGAAAATAAAGATACTG
TTCTGAAACAAAGCACTAGTGAAAATAGTAGTTTGTCCAAATTAGAAGATGTCCAGGTCAAAGATGGAGATAATTCCAAA
AATAAAGGTCCTGTATATGTCGATATAAAAGGTGCTGTTAAACATCCTAATGTTTATAAAATGACATCTAAGGATAGAGT
AGTTGATTTACTTGATAAAGCACAATTATTAGATGATGCAGATGTAAGTCAAATTAATTTGTCTGAAAAATTAACAGATC
AAAAAATGATTTTCATTCCTCATAAAGGACAAAAGAATGTTGAACCACAATTTGGAGCAAACAGTGTGCACGTAAAAAAT
GGGAACACAAATAATACTAAAGTAAATTTAAATACGGCATCTGTATCAGAATTGATGTCTGTTCCTGGAGTAGGGCAAGC
TAAAGCTAATGCAATTGTTGAATATCGCAACCAACAAGGTGCATTTCAAGAAATTGACGATTTGAAAAAAGTAAAAGGTT
TTGGAAGTAAAACTTTTGATAAACTGAATTCTTATTTCACGACATAA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(164-227)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0E1X4U4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Staphylococcus aureus MW2

95.595

99.561

0.952
  comEA Staphylococcus aureus N315

95.595

99.561

0.952

Multiple sequence alignment