Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   E3S86_RS02300 Genome accession   NZ_CP047837
Coordinates   427029..427526 (+) Length   165 a.a.
NCBI ID   WP_001796472.1    Uniprot ID   A0A5F0HJ55
Organism   Staphylococcus aureus strain UP_719     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 422029..432526
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E3S86_RS02260 (E3S86_02260) - 422073..423059 (+) 987 WP_000161307.1 ROK family glucokinase -
  E3S86_RS02265 (E3S86_02265) - 423059..423388 (+) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  E3S86_RS02270 (E3S86_02270) - 423385..424008 (+) 624 WP_001223009.1 MBL fold metallo-hydrolase -
  E3S86_RS02275 (E3S86_02275) comGA 424060..425034 (+) 975 WP_000697220.1 competence type IV pilus ATPase ComGA Machinery gene
  E3S86_RS02280 (E3S86_02280) comGB 425006..426076 (+) 1071 WP_000776409.1 competence type IV pilus assembly protein ComGB Machinery gene
  E3S86_RS02285 (E3S86_02285) comGC 426090..426401 (+) 312 WP_000472255.1 competence type IV pilus major pilin ComGC Machinery gene
  E3S86_RS02290 (E3S86_02290) comGD 426379..426825 (+) 447 WP_001796473.1 competence type IV pilus minor pilin ComGD Machinery gene
  E3S86_RS02295 (E3S86_02295) comGE 426812..427111 (+) 300 WP_000844413.1 hypothetical protein Machinery gene
  E3S86_RS02300 (E3S86_02300) comGF 427029..427526 (+) 498 WP_001796472.1 competence type IV pilus minor pilin ComGF Machinery gene
  E3S86_RS02305 (E3S86_02305) - 427623..427769 (+) 147 WP_001789879.1 hypothetical protein -
  E3S86_RS02310 (E3S86_02310) - 427759..428283 (+) 525 WP_001015120.1 shikimate kinase -
  E3S86_RS02315 (E3S86_02315) gcvT 428442..429533 (+) 1092 WP_000093344.1 glycine cleavage system aminomethyltransferase GcvT -
  E3S86_RS02320 (E3S86_02320) gcvPA 429553..430899 (+) 1347 WP_031928187.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  E3S86_RS02325 (E3S86_02325) gcvPB 430892..432364 (+) 1473 WP_000202176.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPB -

Sequence

Protein


Download         Length: 165 a.a.        Molecular weight: 19169.89 Da        Isoelectric Point: 10.1358

>NTDB_id=417509 E3S86_RS02300 WP_001796472.1 427029..427526(+) (comGF) [Staphylococcus aureus strain UP_719]
MILSKVTNKYVPFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISITLLIVPDLIRLSKTFLIESRELTTVDFEFFSRDI
LEDFKGVDKNDIEIRQQRIILHKGEEMIEYKLINNKIIKVVNDRGNITMINNVTAFTANIYYKSIIKITITVKVGTNLQT
KTIYV

Nucleotide


Download         Length: 498 bp        

>NTDB_id=417509 E3S86_RS02300 WP_001796472.1 427029..427526(+) (comGF) [Staphylococcus aureus strain UP_719]
ATGATATTAAGCAAAGTGACCAACAAATATGTGCCATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTTATTGAAATGTTAGTAGCGATGATGGTTATAAGTATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAGCAAAACTTTTCTAATTGAAAGTAGGGAATTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGAGGATTTTAAAGGTGTAGATAAAAACGATATTGAAATTAGGCAACAGCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATAAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCTATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATCTGCAGACT
AAAACAATTTATGTATAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(68-155)

Prokaryotic N-terminal methylation motif (N_methyl)

(28-51)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5F0HJ55

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Staphylococcus aureus N315

98.182

100

0.982
  comGF Staphylococcus aureus MW2

95.758

100

0.958

Multiple sequence alignment