Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   E3T02_RS02400 Genome accession   NZ_CP047822
Coordinates   449116..449613 (+) Length   165 a.a.
NCBI ID   WP_001829055.1    Uniprot ID   -
Organism   Staphylococcus aureus strain UP_1278     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 444116..454613
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E3T02_RS02360 (E3T02_02360) - 444160..445146 (+) 987 WP_000161314.1 ROK family glucokinase -
  E3T02_RS02365 (E3T02_02365) - 445146..445475 (+) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  E3T02_RS02370 (E3T02_02370) - 445472..446095 (+) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  E3T02_RS02375 (E3T02_02375) comGA 446147..447121 (+) 975 WP_000697227.1 competence type IV pilus ATPase ComGA Machinery gene
  E3T02_RS02380 (E3T02_02380) comGB 447093..448163 (+) 1071 WP_000776406.1 competence type IV pilus assembly protein ComGB Machinery gene
  E3T02_RS02385 (E3T02_02385) comGC 448177..448488 (+) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  E3T02_RS02390 (E3T02_02390) comGD 448466..448912 (+) 447 WP_001788899.1 competence type IV pilus minor pilin ComGD Machinery gene
  E3T02_RS02395 (E3T02_02395) comGE 448899..449198 (+) 300 WP_000844413.1 hypothetical protein Machinery gene
  E3T02_RS02400 (E3T02_02400) comGF 449116..449613 (+) 498 WP_001829055.1 competence type IV pilus minor pilin ComGF Machinery gene
  E3T02_RS02405 (E3T02_02405) - 449710..449856 (+) 147 WP_001789879.1 hypothetical protein -
  E3T02_RS02410 (E3T02_02410) - 449846..450370 (+) 525 WP_001015123.1 shikimate kinase -
  E3T02_RS02415 (E3T02_02415) gcvT 450529..451620 (+) 1092 WP_000093349.1 glycine cleavage system aminomethyltransferase GcvT -
  E3T02_RS02420 (E3T02_02420) gcvPA 451640..452986 (+) 1347 WP_043854778.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  E3T02_RS02425 (E3T02_02425) gcvPB 452979..454451 (+) 1473 WP_000202188.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPB -

Sequence

Protein


Download         Length: 165 a.a.        Molecular weight: 19183.92 Da        Isoelectric Point: 10.2192

>NTDB_id=417260 E3T02_RS02400 WP_001829055.1 449116..449613(+) (comGF) [Staphylococcus aureus strain UP_1278]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISITLLIVPDLIRLSKTFLIESRELTTVDFEFFSRDI
LEDFKGVDRNDIEIRQQRIILHKGEEMIEYKLINNKIIKVVNDRGNITMINNVTAFTANIYYKSIIKITITVKVGTNVQT
KTIYV

Nucleotide


Download         Length: 498 bp        

>NTDB_id=417260 E3T02_RS02400 WP_001829055.1 449116..449613(+) (comGF) [Staphylococcus aureus strain UP_1278]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTCATTGAAATGTTAGTAGCGATGATGGTTATAAGTATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAGCAAAACTTTTCTAATTGAAAGTAGGGAATTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGAGGATTTTAAAGGAGTAGATAGAAACGATATTGAAATTAGGCAACAGCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATAAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCCATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATGTGCAGACT
AAAACTATTTATGTATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(28-51)

Putative Competence protein ComGF (ComGF)

(68-155)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Staphylococcus aureus N315

98.182

100

0.982
  comGF Staphylococcus aureus MW2

98.182

100

0.982

Multiple sequence alignment