Detailed information    

insolico Bioinformatically predicted

Overview

Name   dprA   Type   Machinery gene
Locus tag   H9210_RS02465 Genome accession   NZ_CP060873
Coordinates   512497..513621 (+) Length   374 a.a.
NCBI ID   WP_000228507.1    Uniprot ID   -
Organism   Escherichia coli strain EC27     
Function   ssDNA binding; loading RecA onto ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 507497..518621
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H9210_RS02440 (H9210_02415) mscL 507623..508033 (-) 411 WP_000022442.1 large-conductance mechanosensitive channel protein MscL -
  H9210_RS02445 (H9210_02420) trkA 508163..509539 (-) 1377 WP_000691382.1 Trk system potassium transporter TrkA -
  H9210_RS02450 (H9210_02425) rsmB 509561..510850 (-) 1290 WP_001323790.1 16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB -
  H9210_RS02455 (H9210_02430) fmt 510896..511843 (-) 948 WP_001375336.1 methionyl-tRNA formyltransferase -
  H9210_RS02460 (H9210_02435) def 511858..512367 (-) 510 WP_000114984.1 peptide deformylase -
  H9210_RS02465 (H9210_02440) dprA 512497..513621 (+) 1125 WP_000228507.1 DNA-protecting protein DprA Machinery gene
  H9210_RS02470 (H9210_02445) smg 513593..514066 (+) 474 WP_000460672.1 DUF494 family protein Smg -
  H9210_RS02475 (H9210_02450) yrdD 514095..514637 (+) 543 WP_001129719.1 type I DNA topoisomerase -
  H9210_RS02480 (H9210_02455) tsaC 514642..515214 (+) 573 WP_001297709.1 L-threonylcarbamoyladenylate synthase type 1 TsaC -
  H9210_RS02485 (H9210_02460) aroE 515219..516037 (+) 819 WP_000451239.1 shikimate dehydrogenase -
  H9210_RS02490 (H9210_02465) yrdB 516034..516291 (+) 258 WP_001070563.1 DUF1488 domain-containing protein -
  H9210_RS02495 (H9210_02470) yrdA 516267..516821 (-) 555 WP_001286216.1 gamma carbonic anhydrase family protein -

Sequence

Protein


Download         Length: 374 a.a.        Molecular weight: 40991.98 Da        Isoelectric Point: 6.3837

>NTDB_id=417107 H9210_RS02465 WP_000228507.1 512497..513621(+) (dprA) [Escherichia coli strain EC27]
MVDTDIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGLTLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSE
FYPPQLLVTTDYPGALFVEGELHALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
GVSIAVLGNGLNTIHPRRHARLAASLLEHGGALVSEFPLDVPPLAYNFPRRNRIISGLSKGVLVVEAALRSGSLVTARCA
LEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEPEEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDE
VTPVDVVAERAGQPVPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV

Nucleotide


Download         Length: 1125 bp        

>NTDB_id=417107 H9210_RS02465 WP_000228507.1 512497..513621(+) (dprA) [Escherichia coli strain EC27]
ATGGTCGATACAGATATTTGGCTGCGTTTAATGAGCATCAGCAGCTTGTACGGCGATGATATGGTCCGTATAGCTCACTG
GCTGGCAAAACAGTCGCATATTGATGCGGTTGTATTGCAGCAAACAGGGCTTACATTGCGGCAGGCACAACGCTTTCTTT
CATTTCCGCGAAAGAGTATCGAAAGCTCACTTTGTTGGTTGGAGCAACCCAACCATCATTTAATCCCTGCGGACAGCGAA
TTTTATCCTCCTCAACTTCTGGTGACGACAGATTACCCCGGCGCACTGTTTGTTGAAGGAGAACTGCACGCGCTGCATTC
ATTTCAGCTTGCCGTAGTGGGGAGTCGGGCGCATTCATGGTATGGCGAGCGATGGGGACGGTTATTTTGCGAAACTCTGG
CGACGCGTGGAGTGACAATTACGAGTGGACTGGCGCGTGGAATCGATGGTGTGGCGCATAAAGCTGCCTTACAGGTAAAT
GGCGTCAGCATTGCTGTATTGGGGAATGGACTTAATACCATTCATCCCCGCCGCCATGCCCGACTGGCTGCCAGTCTGCT
TGAACATGGCGGAGCTCTCGTCTCGGAATTTCCCCTCGATGTTCCACCCCTTGCTTACAATTTCCCACGAAGAAATCGCA
TTATCAGTGGTCTAAGTAAAGGTGTACTGGTGGTGGAAGCGGCTTTGCGCAGTGGTTCGCTGGTGACAGCACGTTGTGCG
CTTGAGCAGGGGCGTGAAGTTTTTGCCTTGCCAGGTCCAATAGGGAATCCGGGAAGCGAAGGGCCTCACTGGTTAATAAA
ACAAGGTGCGATTCTTGTGACGGAACCGGAAGAAATTCTGGAAAACTTGCAATTTGGATTGCACTGGTTGCCAGACGCCC
CTGAAAATTCATTTTATTCACCAGATCAGCAAGACGTGGCATTGCCATTTCCTGAGCTCCTGGCTAACGTAGGAGATGAG
GTAACACCTGTTGACGTCGTCGCTGAACGTGCCGGCCAACCTGTGCCAGAGGTAGTTACTCAACTACTCGAACTGGAGTT
AGCAGGATGGATCGCAGCTGTACCCGGCGGCTATGTCCGATTGAGGAGGGCATGCCATGTTCGACGTACTAATGTATTTG
TTTGA

Domains

Predicted by InterProScan.

DNA recombination-mediator protein A (DNA_processg_A)

(67-274)

DprA winged helix domain (WHD_DprA)

(312-357)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  dprA Vibrio campbellii strain DS40M4

48.509

98.663

0.479
  dprA Vibrio cholerae O1 biovar El Tor strain E7946

44.652

100

0.447
  dprA Vibrio cholerae strain A1552

44.652

100

0.447
  dprA Haemophilus influenzae Rd KW20

45.401

90.107

0.409
  dprA Glaesserella parasuis strain SC1401

44.807

90.107

0.404
  dprA Legionella pneumophila strain ERS1305867

44.242

88.235

0.39

Multiple sequence alignment