Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   E3T14_RS03525 Genome accession   NZ_CP047809
Coordinates   731643..732617 (-) Length   324 a.a.
NCBI ID   WP_000697228.1    Uniprot ID   A0A0D3Q868
Organism   Staphylococcus aureus strain UP_1591     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 726643..737617
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E3T14_RS03485 (E3T14_03490) gcvT 727144..728235 (-) 1092 WP_000093349.1 glycine cleavage system aminomethyltransferase GcvT -
  E3T14_RS03490 (E3T14_03495) - 728394..728918 (-) 525 WP_001015117.1 shikimate kinase -
  E3T14_RS03495 (E3T14_03500) - 728908..729054 (-) 147 WP_001789879.1 hypothetical protein -
  E3T14_RS03500 (E3T14_03505) comGF 729151..729648 (-) 498 WP_001789864.1 competence type IV pilus minor pilin ComGF Machinery gene
  E3T14_RS03505 (E3T14_03510) comGE 729566..729865 (-) 300 WP_000844413.1 hypothetical protein Machinery gene
  E3T14_RS03510 (E3T14_03515) comGD 729852..730298 (-) 447 WP_001790850.1 competence type IV pilus minor pilin ComGD Machinery gene
  E3T14_RS03515 (E3T14_03520) comGC 730276..730587 (-) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  E3T14_RS03520 (E3T14_03525) comGB 730601..731671 (-) 1071 Protein_696 competence type IV pilus assembly protein ComGB -
  E3T14_RS03525 (E3T14_03530) comGA 731643..732617 (-) 975 WP_000697228.1 competence type IV pilus ATPase ComGA Machinery gene
  E3T14_RS03530 (E3T14_03535) - 732669..733292 (-) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  E3T14_RS03535 (E3T14_03540) - 733289..733618 (-) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  E3T14_RS03540 (E3T14_03545) - 733618..734604 (-) 987 WP_000161314.1 ROK family glucokinase -
  E3T14_RS03545 (E3T14_03550) - 734601..734804 (-) 204 WP_000087561.1 YqgQ family protein -
  E3T14_RS03550 (E3T14_03555) - 734785..736248 (-) 1464 WP_001017863.1 rhomboid family intramembrane serine protease -
  E3T14_RS03555 (E3T14_03560) - 736260..736799 (-) 540 WP_000164545.1 5-formyltetrahydrofolate cyclo-ligase -
  E3T14_RS03560 (E3T14_03565) rpmG 737008..737157 (-) 150 WP_001265709.1 50S ribosomal protein L33 -

Sequence

Protein


Download         Length: 324 a.a.        Molecular weight: 36865.01 Da        Isoelectric Point: 9.0399

>NTDB_id=417007 E3T14_RS03525 WP_000697228.1 731643..732617(-) (comGA) [Staphylococcus aureus strain UP_1591]
MKILFQEIINKAIEMKASDVHFIPVKNEVSIKFRINDNLEQYEQIGNSIYQKLLVYMKFQAGLDVSTQQVAQSGRYSYLF
NKIYFLRISTLPLSLGQESCVIRIVPQFFQPQKSTYKFNDFKHLMNKKQGLLLFSGPTGSGKSTLMYQMVSYANKALNLN
VISIEDPVEMQIPGIVQINVNDKAGINYVNSFKAILRCDPDVILIGEIRDKDVAKCVIQASLSGHLVLTTLHATDCKGAI
LRLLEMGISVQELIQATNLIINQRLVTTIKQQRQLVCEILSQQQLRYFFSHNHSLPSSFKNLEDKLDDMTKAGVICETTM
HKYI

Nucleotide


Download         Length: 975 bp        

>NTDB_id=417007 E3T14_RS03525 WP_000697228.1 731643..732617(-) (comGA) [Staphylococcus aureus strain UP_1591]
TTGAAGATTCTATTTCAAGAAATAATTAATAAAGCGATAGAAATGAAAGCGAGTGATGTACATTTTATTCCAGTTAAAAA
TGAAGTAAGTATTAAATTTAGAATTAATGATAACTTGGAGCAGTATGAACAAATTGGGAATAGCATTTATCAAAAGTTAT
TAGTTTATATGAAGTTTCAAGCTGGGCTTGATGTTTCTACACAGCAAGTCGCACAGAGCGGTCGATATAGTTACCTTTTC
AATAAAATATATTTTTTGAGAATATCAACTTTACCATTGTCACTTGGCCAAGAAAGTTGTGTTATCAGAATTGTACCTCA
ATTTTTTCAACCACAGAAATCAACTTATAAATTCAATGATTTTAAACACCTCATGAATAAGAAACAAGGATTACTATTGT
TTAGTGGACCAACTGGTTCAGGAAAGAGTACATTAATGTATCAAATGGTCTCATACGCGAATAAAGCCTTGAATTTAAAT
GTAATTTCTATAGAGGATCCTGTAGAGATGCAAATTCCTGGTATCGTCCAAATTAATGTGAATGATAAAGCTGGCATAAA
CTATGTAAATTCGTTTAAAGCTATTTTAAGATGTGATCCTGATGTTATTTTAATAGGTGAAATCAGAGATAAAGATGTTG
CCAAGTGTGTTATACAGGCTAGTTTAAGTGGTCACCTTGTTCTGACTACATTGCATGCAACTGATTGTAAAGGTGCTATT
TTAAGGCTATTAGAAATGGGCATTTCTGTACAAGAATTGATACAGGCAACTAACTTAATTATAAACCAACGACTTGTAAC
TACTATTAAGCAACAGCGACAATTAGTATGTGAAATTCTATCTCAGCAACAACTCCGATATTTCTTTTCCCATAATCATT
CATTACCATCATCATTTAAGAACTTAGAAGATAAACTTGATGATATGACAAAAGCAGGTGTCATTTGTGAAACTACAATG
CATAAATACATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0D3Q868

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Staphylococcus aureus MW2

99.074

100

0.991
  comGA Staphylococcus aureus N315

99.074

100

0.991

Multiple sequence alignment