Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   E3S75_RS02035 Genome accession   NZ_CP047798
Coordinates   379415..380101 (+) Length   228 a.a.
NCBI ID   WP_072468915.1    Uniprot ID   A0A5F0HFN7
Organism   Staphylococcus aureus strain UP_378     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 374415..385101
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E3S75_RS02000 (E3S75_02000) yqeH 374942..376042 (+) 1101 WP_001280146.1 ribosome biogenesis GTPase YqeH -
  E3S75_RS02005 (E3S75_02005) aroE 376056..376862 (+) 807 WP_000666750.1 shikimate dehydrogenase -
  E3S75_RS02010 (E3S75_02010) yhbY 376866..377156 (+) 291 WP_000955234.1 ribosome assembly RNA-binding protein YhbY -
  E3S75_RS02015 (E3S75_02015) nadD 377159..377728 (+) 570 WP_000725165.1 nicotinate (nicotinamide) nucleotide adenylyltransferase -
  E3S75_RS02020 (E3S75_02020) yqeK 377718..378302 (+) 585 WP_001017837.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  E3S75_RS02025 (E3S75_02025) rsfS 378303..378656 (+) 354 WP_001088020.1 ribosome silencing factor -
  E3S75_RS02030 (E3S75_02030) - 378659..379375 (+) 717 WP_000084840.1 class I SAM-dependent DNA methyltransferase -
  E3S75_RS02035 (E3S75_02035) comEA 379415..380101 (+) 687 WP_072468915.1 ComEA family DNA-binding protein Machinery gene
  E3S75_RS02040 (E3S75_02040) - 380193..380654 (+) 462 WP_000439693.1 ComE operon protein 2 -
  E3S75_RS02045 (E3S75_02045) comEC 380659..382860 (+) 2202 WP_001622275.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  E3S75_RS02050 (E3S75_02050) holA 382917..383891 (+) 975 WP_001282573.1 DNA polymerase III subunit delta -
  E3S75_RS02055 (E3S75_02055) rpsT 383936..384187 (-) 252 WP_001274017.1 30S ribosomal protein S20 -

Sequence

Protein


Download         Length: 228 a.a.        Molecular weight: 25812.41 Da        Isoelectric Point: 9.3305

>NTDB_id=416619 E3S75_RS02035 WP_072468915.1 379415..380101(+) (comEA) [Staphylococcus aureus strain UP_378]
MVLLYQFLLRYKDFLTQWKLYIISAVVLIMVLIGFIFWRQDDYTSRDFENKDTALKQSTSENSSLSKLEDVQAKDGDNSK
NKGPVYVDIKGAVKHPNVYKMTSKDRVVDLLDKAQLLDDADVSQINLSEKLTDQKMIFIPHKGQKNVEPQFGENSVHVKN
GNTNNTKVNLNTASVSELMSVPGVGQAKANAIVEYRNQQGAFQEIDDLKKVKGFGSKTFDKLKSYFTT

Nucleotide


Download         Length: 687 bp        

>NTDB_id=416619 E3S75_RS02035 WP_072468915.1 379415..380101(+) (comEA) [Staphylococcus aureus strain UP_378]
GTGGTTTTATTGTATCAATTTTTATTACGCTATAAAGATTTTTTAACTCAATGGAAGTTATATATTATAAGTGCTGTTGT
TTTAATTATGGTATTAATTGGTTTTATATTCTGGAGACAAGATGATTATACTTCAAGAGATTTTGAAAATAAAGATACTG
CTCTGAAACAAAGCACTAGTGAAAATAGTAGTTTGTCCAAATTAGAAGATGTCCAGGCCAAAGATGGAGATAATTCCAAA
AATAAAGGTCCTGTATATGTCGATATAAAAGGTGCTGTTAAACACCCTAATGTTTATAAAATGACATCTAAGGATAGAGT
AGTTGATTTACTTGATAAAGCACAATTATTGGATGATGCAGATGTAAGTCAAATTAATTTGTCTGAAAAATTAACAGATC
AAAAAATGATTTTCATTCCTCATAAAGGACAAAAGAATGTTGAACCACAATTTGGAGAAAACAGTGTGCACGTAAAAAAT
GGGAACACAAATAATACTAAAGTAAATTTAAATACGGCATCTGTATCAGAATTGATGTCTGTTCCTGGAGTAGGGCAAGC
TAAAGCTAATGCAATTGTTGAATATCGCAACCAACAAGGTGCATTTCAAGAAATTGACGATTTGAAAAAAGTAAAAGGTT
TTGGAAGTAAAACTTTTGATAAACTGAAATCTTATTTCACGACATAA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(164-227)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5F0HFN7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Staphylococcus aureus MW2

96.476

99.561

0.961
  comEA Staphylococcus aureus N315

96.476

99.561

0.961

Multiple sequence alignment