Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   E3S77_RS02440 Genome accession   NZ_CP047797
Coordinates   462103..462600 (+) Length   165 a.a.
NCBI ID   WP_001825340.1    Uniprot ID   A0A2S6DDC2
Organism   Staphylococcus aureus strain UP_426     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 457103..467600
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E3S77_RS02400 (E3S77_02400) - 457147..458133 (+) 987 WP_000161315.1 ROK family glucokinase -
  E3S77_RS02405 (E3S77_02405) - 458133..458462 (+) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  E3S77_RS02410 (E3S77_02410) - 458459..459082 (+) 624 WP_047213494.1 MBL fold metallo-hydrolase -
  E3S77_RS02415 (E3S77_02415) comGA 459134..460108 (+) 975 WP_000697222.1 competence type IV pilus ATPase ComGA Machinery gene
  E3S77_RS02420 (E3S77_02420) comGB 460080..461150 (+) 1071 WP_000776413.1 competence type IV pilus assembly protein ComGB Machinery gene
  E3S77_RS02425 (E3S77_02425) comGC 461164..461475 (+) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  E3S77_RS02430 (E3S77_02430) comGD 461453..461899 (+) 447 WP_072357807.1 competence type IV pilus minor pilin ComGD Machinery gene
  E3S77_RS02435 (E3S77_02435) comGE 461886..462185 (+) 300 WP_000844415.1 hypothetical protein Machinery gene
  E3S77_RS02440 (E3S77_02440) comGF 462103..462600 (+) 498 WP_001825340.1 competence type IV pilus minor pilin ComGF Machinery gene
  E3S77_RS02445 (E3S77_02445) - 462697..462843 (+) 147 WP_001789879.1 hypothetical protein -
  E3S77_RS02450 (E3S77_02450) - 462833..463357 (+) 525 WP_001015120.1 shikimate kinase -
  E3S77_RS02455 (E3S77_02455) gcvT 463516..464607 (+) 1092 WP_000093354.1 glycine cleavage system aminomethyltransferase GcvT -
  E3S77_RS02460 (E3S77_02460) gcvPA 464627..465973 (+) 1347 WP_000019687.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  E3S77_RS02465 (E3S77_02465) gcvPB 465966..467438 (+) 1473 WP_000202177.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPB -

Sequence

Protein


Download         Length: 165 a.a.        Molecular weight: 19179.92 Da        Isoelectric Point: 10.2192

>NTDB_id=416587 E3S77_RS02440 WP_001825340.1 462103..462600(+) (comGF) [Staphylococcus aureus strain UP_426]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISITLLIVPDLIRLSKTFLIESRELTTVDFEFFSRDI
LEDFKGVDRNDIEIRQQRIILHKGEEMIEYKLINNKIIKVVNDRGNITLINNVTAFTANIYYKSIIKITITVKVGTNLQT
KTIYV

Nucleotide


Download         Length: 498 bp        

>NTDB_id=416587 E3S77_RS02440 WP_001825340.1 462103..462600(+) (comGF) [Staphylococcus aureus strain UP_426]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTTATTGAAATGTTAGTAGCGATGATGGTTATAAGTATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAGCAAAACTTTTCTAATTGAAAGTAGGGAATTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGAGGATTTTAAAGGAGTAGATAGAAACGATATTGAAATTAGGCAACAGCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATAAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACATTGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCTATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATCTGCAGACT
AAAACTATTTATGTATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(28-51)

Putative Competence protein ComGF (ComGF)

(68-155)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2S6DDC2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Staphylococcus aureus N315

98.182

100

0.982
  comGF Staphylococcus aureus MW2

96.97

100

0.97

Multiple sequence alignment