Detailed information NTG0041625

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: CNE_RS15600
• Genome accession: NC_015726
• Coordinates: 3327756..3328658 (+)
• Length: 300 a.a.
• NCBI ID: WP_013958060.1
• Uniprot ID: G0EX70
• Organism: Cupriavidus necator N-1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3322756..3333658

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
CNE_RS15575 (CNE_1c31890)	ispB	3322901..3323830 (+)	930	WP_049800579.1	octaprenyl diphosphate synthase	-
CNE_RS15585 (CNE_1c31910)	-	3324110..3324430 (-)	321	WP_013958057.1	helix-turn-helix domain-containing protein	-
CNE_RS15590 (CNE_1c31920)	pilF	3324671..3326401 (+)	1731	WP_013958058.1	type IV-A pilus assembly ATPase PilB	Machinery gene
CNE_RS15595 (CNE_1c31930)	-	3326481..3327752 (+)	1272	WP_013958059.1	type II secretion system F family protein	-
CNE_RS15600 (CNE_1c31940)	pilD	3327756..3328658 (+)	903	WP_013958060.1	A24 family peptidase	Machinery gene
CNE_RS15605 (CNE_1c31950)	coaE	3328722..3329348 (+)	627	WP_013958061.1	dephospho-CoA kinase	-
CNE_RS15610 (CNE_1c31960)	zapD	3329558..3330316 (+)	759	WP_035826777.1	cell division protein ZapD	-
CNE_RS15615 (CNE_1c31970)	-	3330334..3330522 (+)	189	WP_013958063.1	DNA gyrase inhibitor YacG	-
CNE_RS15620	-	3330554..3332593 (-)	2040	Protein_3135	OPT family oligopeptide transporter	-
CNE_RS15625 (CNE_1c31990)	-	3332688..3333134 (-)	447	WP_013958064.1	NUDIX domain-containing protein	-

Sequence

Protein

• Length: 300 a.a.
• Molecular weight: 32083.30 Da
• Isoelectric Point: 8.0346

>NTDB_id=41625 CNE_RS15600 WP_013958060.1 3327756..3328658(+) (pilD) [Cupriavidus necator N-1]
MLSAWSASPYPTGGAPLVEALAALPPAFLVAAAALLGLVVGSFLNVVIHRMPRMMERDEANYIAELRGDPLPHPGRYNLM
VPRSACPHCGHAIAPWENVPVLSYLFLRGRCSACKAPISVRYPLVELACGVLSALVAWRFGPGGQALAALVLVWALLALT
MIDADTQLLPDQITLPLLWLGLLLNLGGVFVALPDAVIGAAAGYLVLWTAYWLFRLVRGKEGMGFGDFKLMAALGAWFGW
QALPALVLLSSVVGVLFGLANIVLRRQERDTPFPFGPFIALAGVVVLLFGPGVLPLFAWP

Nucleotide

• Length: 903 bp

>NTDB_id=41625 CNE_RS15600 WP_013958060.1 3327756..3328658(+) (pilD) [Cupriavidus necator N-1]
ATGTTGTCTGCGTGGTCTGCTTCTCCGTATCCGACCGGTGGCGCGCCGCTGGTGGAGGCACTGGCGGCGCTGCCGCCCGC
CTTTCTGGTGGCTGCCGCGGCGCTGCTCGGGCTGGTGGTCGGCAGCTTCCTCAACGTGGTCATCCACCGGATGCCGCGCA
TGATGGAGCGCGACGAGGCCAACTATATCGCCGAGCTGCGCGGCGACCCGCTGCCCCACCCCGGCCGCTACAACCTGATG
GTGCCGCGCTCGGCCTGCCCGCATTGCGGCCACGCGATTGCACCCTGGGAGAACGTGCCGGTGCTGAGCTACCTGTTCCT
GCGCGGGCGCTGCTCGGCATGCAAGGCGCCGATCAGTGTGCGCTATCCGCTGGTCGAGCTGGCCTGTGGCGTGCTGAGCG
CGCTGGTGGCGTGGCGCTTCGGCCCCGGCGGACAGGCCCTGGCCGCGCTGGTGCTGGTGTGGGCGCTGCTGGCGCTGACC
ATGATCGATGCCGATACGCAACTGTTGCCGGACCAGATCACCCTGCCGCTGCTGTGGCTGGGCCTGCTGCTGAACCTGGG
CGGCGTCTTCGTGGCGCTGCCCGACGCCGTGATCGGCGCGGCTGCCGGCTACCTGGTGCTGTGGACGGCCTACTGGCTGT
TCCGGCTCGTGCGCGGCAAGGAAGGCATGGGCTTTGGCGACTTCAAGCTGATGGCGGCACTGGGCGCCTGGTTCGGCTGG
CAGGCGCTGCCGGCGCTGGTGCTGCTGTCGTCGGTGGTCGGCGTGCTGTTCGGGCTGGCCAATATTGTGCTGCGCCGCCA
GGAACGCGACACGCCCTTCCCGTTCGGACCCTTCATCGCGCTGGCCGGGGTGGTGGTGCTGCTGTTCGGCCCCGGCGTGC
TGCCGCTGTTTGCATGGCCCTGA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (35-140)

• Type IV leader peptidase family (Peptidase_A24) (150-259)

3D structure

• AlphaFold DB: G0EX70

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	55.849	88.333	0.493
pilD	Vibrio campbellii strain DS40M4	51.095	91.333	0.467
pilD	Neisseria gonorrhoeae MS11	48.239	94.667	0.457
pilD	Acinetobacter baumannii D1279779	47.601	90.333	0.43
pilD	Acinetobacter nosocomialis M2	47.232	90.333	0.427