Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   E3T00_RS07345 Genome accession   NZ_CP047784
Coordinates   1541561..1542535 (-) Length   324 a.a.
NCBI ID   WP_000697227.1    Uniprot ID   -
Organism   Staphylococcus aureus strain UP_1209     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1536561..1547535
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E3T00_RS07305 (E3T00_07305) gcvT 1537062..1538153 (-) 1092 WP_000093345.1 glycine cleavage system aminomethyltransferase GcvT -
  E3T00_RS07310 (E3T00_07310) - 1538312..1538836 (-) 525 WP_001015121.1 shikimate kinase -
  E3T00_RS07315 (E3T00_07315) - 1538826..1538972 (-) 147 WP_001789879.1 hypothetical protein -
  E3T00_RS07320 (E3T00_07320) comGF 1539069..1539566 (-) 498 WP_001829055.1 competence type IV pilus minor pilin ComGF Machinery gene
  E3T00_RS07325 (E3T00_07325) comGE 1539484..1539783 (-) 300 WP_000844413.1 hypothetical protein Machinery gene
  E3T00_RS07330 (E3T00_07330) comGD 1539770..1540216 (-) 447 WP_001788899.1 competence type IV pilus minor pilin ComGD Machinery gene
  E3T00_RS07335 (E3T00_07335) comGC 1540194..1540505 (-) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  E3T00_RS07340 (E3T00_07340) comGB 1540519..1541589 (-) 1071 WP_160198952.1 competence type IV pilus assembly protein ComGB Machinery gene
  E3T00_RS07345 (E3T00_07345) comGA 1541561..1542535 (-) 975 WP_000697227.1 competence type IV pilus ATPase ComGA Machinery gene
  E3T00_RS07350 (E3T00_07350) - 1542587..1543210 (-) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  E3T00_RS07355 (E3T00_07355) - 1543207..1543536 (-) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  E3T00_RS07360 (E3T00_07360) - 1543536..1544522 (-) 987 WP_000161314.1 ROK family glucokinase -
  E3T00_RS07365 (E3T00_07365) - 1544519..1544722 (-) 204 WP_000087561.1 YqgQ family protein -
  E3T00_RS07370 (E3T00_07370) - 1544703..1546166 (-) 1464 WP_001017867.1 rhomboid family protein -
  E3T00_RS07375 (E3T00_07375) - 1546178..1546717 (-) 540 WP_000164546.1 5-formyltetrahydrofolate cyclo-ligase -
  E3T00_RS07380 (E3T00_07380) rpmG 1546926..1547075 (-) 150 WP_001265709.1 50S ribosomal protein L33 -

Sequence

Protein


Download         Length: 324 a.a.        Molecular weight: 36842.96 Da        Isoelectric Point: 8.8508

>NTDB_id=416050 E3T00_RS07345 WP_000697227.1 1541561..1542535(-) (comGA) [Staphylococcus aureus strain UP_1209]
MKILFQEIINKAIEMKASDVHFIPVKNEVSIKFRINDNLEQYEQIGNSIYQKLLVYMKFQAGLDVSTQQVAQSGRYSYLF
NKIYFLRISTLPLSLGQESCVIRIVPQFFQPQKSTYKFNDFKHLMNKKQGLLLFSGPTGSGKSTLMYQMVSYANKALNLN
VISIEDPVEMQIPGIVQINVNDKAGINYVNSFKAILRCDPDVILIGEIRDKDVAKCVIQASLSGHLVLTTLHATDCKGAI
LRLLEMGISVQELIQATNLIINQRLVTTIKQQRQLVCEILSQQQLRYFFSHNHSLPSSFKNLEDKLDDMTKAGVICETTM
DKYI

Nucleotide


Download         Length: 975 bp        

>NTDB_id=416050 E3T00_RS07345 WP_000697227.1 1541561..1542535(-) (comGA) [Staphylococcus aureus strain UP_1209]
TTGAAGATTCTATTTCAAGAAATAATTAATAAAGCGATAGAAATGAAAGCGAGTGATGTACATTTTATTCCAGTTAAAAA
TGAAGTAAGTATTAAATTTAGAATTAATGATAACTTGGAGCAGTATGAACAAATTGGGAATAGCATTTATCAAAAGTTAT
TAGTTTATATGAAGTTTCAAGCTGGGCTTGATGTTTCTACACAGCAAGTCGCACAGAGCGGTCGATATAGTTACCTTTTC
AATAAAATATATTTTTTGAGAATATCAACTTTACCATTGTCACTTGGCCAAGAAAGTTGTGTTATCAGAATTGTACCTCA
ATTTTTTCAACCACAGAAATCAACTTATAAATTCAATGATTTTAAACACCTCATGAATAAGAAACAAGGATTACTATTGT
TTAGTGGACCAACTGGTTCAGGAAAGAGTACATTAATGTATCAAATGGTCTCATACGCGAATAAAGCCTTGAATTTAAAT
GTAATTTCTATAGAGGATCCTGTAGAGATGCAAATTCCTGGTATCGTCCAAATTAATGTGAATGATAAAGCTGGCATTAA
CTATGTAAATTCGTTTAAAGCTATTTTAAGATGTGATCCTGATGTTATTTTAATAGGTGAAATCAGAGATAAAGATGTTG
CCAAGTGTGTTATACAGGCTAGTTTAAGTGGTCACCTTGTTCTGACTACATTGCATGCAACTGATTGTAAAGGTGCTATT
TTAAGGCTATTAGAAATGGGTATTTCTGTACAAGAATTGATACAGGCAACTAACTTAATTATAAACCAACGACTTGTAAC
TACTATTAAGCAACAGCGACAATTAGTATGTGAAATTCTATCTCAGCAACAACTCCGATATTTCTTTTCCCATAATCATT
CATTACCATCATCATTTAAGAACTTAGAAGATAAACTTGATGATATGACAAAAGCAGGTGTCATTTGTGAAACTACAATG
GATAAATACATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Staphylococcus aureus MW2

99.383

100

0.994
  comGA Staphylococcus aureus N315

99.383

100

0.994

Multiple sequence alignment